摘要
以枸杞汁为原料,采取响应面法对壳聚糖固定化复合酶澄清枸杞汁的工艺进行优化。在单因素的基础上,采用三因素三水平的响应曲面优化设计,研究超声温度、超声时间、酶添加量各自变量及其交互作用对枸杞汁透光率的影响,构建了二次工艺多项式回归数学模型。结果表明:超声温度50.51℃,超声时间64.61min,壳聚糖固定化复合酶用量24.03g/L[m(纤维素酶)∶m(果胶酶)=1∶2],透光率84.51%,与响应面预测值(84.60%)基本一致,为实际工业化生产提供理论依据。
The wolfberry juice was used as raw material to optimize the process of chitosan immobilized complex enzyme clarified mash juice by response surface method.Single factor on the use of three factors and three levels of response surface optimization design,ultrasound temperature,sonication time,the addition amount of each enzyme variables and their interactions on wolfberry juice light transmittance established quadratic polynomial regression prediction.The results showed:ultrasonic temperature 50.51℃,ultrasonic time 64.61 min,chitosan immobilized complex enzyme dosage 24.03g/L [m(pectinase)∶m(cellulase)=2∶1].The light transmittance is 84.51%,which is well fitted with the predicted surface response value of 84.60%,which provides a theoretical basis for practical industrial production.
引文
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