摘要
对比了三种提取小麦DON方法的差异。采用振荡、超声和旋涡提取对充分混合的小麦粉状样品进行提取并测定。结果表明:三种提取方法的DON提取率都与提取时间呈正相关,旋涡法的最佳提取时间最短(4 min),超声法的最佳时间次之(15 min),振荡法的最佳时间最长(20 min)。振荡法和旋涡法测得的DON含量分别为(839.7±40.5)μg/kg和(804.8±28.9)μg/kg,两种方法之间无显著差异;超声法测得的DON含量为(760.6±36.0)μg/kg,明显低于其他两种方法。
This experiment compared the three extraction methods of DON in wheat. The fully mixed wheat powder samples were extracted and determined by oscillating extraction ultrasonic extraction and vortex extraction. The results showed that the extraction rates of the three extraction methods were positively correlated with the extraction time. The optimal extraction time of the vortex method was the shortest(4 min), followed by the ultrasonic method(15 min), and the oscillation method was the longest(20 min). The DON content measured by the oscillation method and the vortex method was 839.7±40.5 μg/kg and 804.8±28.9 μg/kg, and there was no significant difference between the two methods. The content of DON measured by ultrasonic method was 760.6±36.0 μg/kg, significantly lower than the other two methods.
引文
1 KUMERA N,ALI M.Mycotoxin occurrence in grains and the role of postharvest management as a mitigation strategies.A review [J].Food Control,2017,78:412～425
2 Amirahmadi M,Shoeibi S,Rastegar Het al,et al.Simultaneous analysis of mycotoxins in corn flour using LC/MS-MS combined with a modified QuEChERS procedure[J].Toxin Reviews,2017:1～9
3 计成,赵丽红,李笑英,等.呕吐毒素生物降解研究进展[J].饲料工业,2015,36(10):487
4 Amin M K,Yadolah F,Anderson S S.Impact of unit operations during processing of cereal-based products on the levels of deoxynivalenol,total aflatoxin,ochratoxin A,and zearalenone:Asystematic review and meta-analysis[J].Food Chemistry,2018,268:611～624
5 WU L,WANG B J.Evaluation on levels and conversion profiles of DON,3-ADON and 15-ADON during bread making process[J].Food Chemistry,2015,185:509～516
6 吴本刚,孙宝胜,徐存宽,等.小麦中呕吐毒素和玉米赤霉烯酮与赤霉病粒关系研究[J].粮食与油脂,2017,30(7):105～108
7 盛林霞,付豪,吴艺影,等.粮食中呕吐毒素检测方法的研究进展[J].粮食储藏,2018(1):32～35
8 杨瑛,郑文轩.超声波降解苯酚废水的研究[J].安徽农业科学,2010,38(34):19454～19455
9 杨龙.臭氧水协同超声波处理对小麦中呕吐毒素的降解效果研究[D].河南工业大学,2018
