摘要
采用双层牛津杯法研究枯草芽孢杆菌CGMCC 6624对抗生素的耐受性。结果显示:当抗生素浓度在1×10-5～1mg/mL时,枯草芽孢杆菌CGMCC 6624对硝基咪唑类中的替硝唑具有耐受性;当抗生素浓度为1×10-3～1mg/mL时,枯草芽孢杆菌CGMCC 6624对四环素类中的四环素和土霉素,喹诺酮类中的氧氟沙星具有耐受性;当抗生素浓度在1×10-2～1mg/mL时,枯草芽孢杆菌CGMCC 6624对β-内酰胺类中的阿莫西林、青霉素和氨苄青霉素,氨基糖苷类中的链霉素,酰胺醇类中的氯霉素、甲砜霉素,大环内酯类中的红霉素和硝基咪唑类中的甲硝唑具有耐受性;当抗生素浓度在1×10-1～1mg/mL时,枯草芽孢杆菌CGMCC 6624对氨基糖苷类中的庆大霉素、新霉素,酰胺类中的氟苯尼考和磺胺类中的磺胺具有耐受性;当抗生素浓度为1mg/mL时,枯草芽孢杆菌CGMCC 6624对大环内酯类中的阿奇霉素具有耐受性。以上结果表明枯草芽孢杆菌CGMCC 6624对不同种类的抗生素具有一定的耐受性。该研究为该菌株在食品、饲料中的应用奠定了理论基础。
The resistance of Bacillus subtilis CGMCC 6624 to antibiotics is researched using doublelayered Oxford cup method.The results show that B.subtilis CGMCC 6624 is resistant to tinidazole in nitroimidazole when the antibiotic concentration is 1×10~(-5)～1 mg/mL;B.subtilis CGMCC 6624 is resistant to tetracycline and oxytetracycline in tetracycline,and ofloxacin in quinolones when the antibiotic concentration is 1×10~(-3)～1 mg/mL;B.subtilis CGMCC 6624 is resistant to amoxicillin,penicillin and ampicillin inβ-lactams,streptomycin in aminoglycosides,chloramphenicol,thiamphenicols in amide alcohols,erythromycin in macrolides and metronidazole in nitroimidazoles;B.subtilis CGMCC 6624 is resistant to gentamicin,neomycin in aminoglycosides,florfenicol in amide and sulfonamide in sulfonamides when the antibiotic concentration is 1×10~(-1)～1 mg/mL;B.subtilis CGMCC 6624 is resistant to azithromycin in macrolides when the antibiotic concentration is 1 mg/mL.The above results indicate that B.subtilis CGMCC 6624 is resistant to different types of antibiotics.This research has laid a theoretical foundation for the application of strains in food and feed.
引文
[1]魏长浩,邓泽元.益生菌及其应用研究进展[J].乳业科学与技术,2018,41(1):26-32.
[2]丁瑞雪,武俊瑞,岳喜庆,等.益生菌对肠道营养健康调控作用的研究进展[J].中国乳品工业,2018,46(1):24-32.
[3]Islam Su.Clinical uses of probiotics[J].Medicine(Baltimore),2016,95(5):e2658.
[4]Sanders M E,Guarner F,Guerrant R,et al.An update on the use and investigation of probiotics in health and disease[J].Gut,2013,62(5):787-796.
[5]Working Group Report.Guidelines for the evaluation of probiotics in food[R].Joint Food and Agriculture Organisation of the United Nations and the World Health Organisation,2007.
[6]蒋磊,徐秀景.益生菌在畜禽生产中的应用[J].饲料广角,2017(1):45-46.
[7]贾丽艳,郝林,段先瑶.枯草芽孢杆菌HJD.A32产细菌素的生物学特性研究[J].中国食品学报,2013,13(4):74-79.
[8]胡敏.枯草芽孢杆菌A32产细菌素发酵条件及其稳定性研究[D].晋中:山西农业大学,2013.
[9]段先瑶.醋醅中具有抑菌作用菌株的分离与筛选及其抑菌物质的研究[D].晋中:山西农业大学,2010.(下转第41页)
[10]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[11]贾丽艳.产类细菌素菌株的筛选鉴定及类细菌素的研究[D].晋中:山西农业大学,2014.
[12]彭小林,范亚新,张亮,等.氨基糖苷类抗生素治疗药物浓度监测的研究进展[J].中国感染与化疗杂志,2017,17(1):104-109.
[13]李喆宇,崔玉彬,张静霞,等.大环内酯类抗生素的研究新进展[J].国外医药(抗生素分册),2013,34(1):6-15.
[14]Prolx M K,Palace S G,Ganddra S,et al.Reversion from methicillin susceptibility to methcillin resistance in Staphylococcus aureus during treatment of bacteremia[J].J Infet Dis,2016,213(6):1041-1048.
[15]徐冬梅,王艳花,饶桂维.四环素类抗生素对淡水绿藻的毒性作用[J].环境科学,2013,34(9):3386-3390.
[16]杨晓芳,杨涛,王莹,等.四环素类抗生素污染现状及其环境行为研究进展[J].环境工程,2014,32(2):123-127.
[17]龙彩虹,李静娜,张涛,等.武汉市市售351件动物源性食品中磺胺类抗生素残留检测[J].现代预防医学,2018,45(6):1001-1004.
[18]王宁.喹诺酮类抗菌药物耐药机制[J].山东畜牧兽医,2015,36(7):74-75.
[19]Schwarz S,Kehrenberg C,Doublet B,et al.Molecular basis of bacterial resistance of chloramphenicol and florfenicol[J].FEMS Microbiol Rev,2004,28(5):519-542.
[20]励炯,孙岚,王红青,等.分散固相萃取净化/高效液相色谱-串联质谱法测定水产品中的5种硝基咪唑类药物残留[J].分析测试学报,2017,36(11):1357-1362.
