摘要
肠膜状明串珠菌发酵生产右旋糖酐。肠膜状明串珠菌发酵液经过板框过滤和陶瓷膜微滤,板框过滤总糖和果糖的收率均在90%以上,菌数从2.05×1014个·m L-1降低到1.61×109个·m L-1;陶瓷膜微滤总糖和果糖的收率均在87%以上,菌数从1.61×109个·m L-1降低到1.06×106个·m L-1。肠膜状明串珠菌发酵液后处理总糖和果糖的收率均在80%以上,菌数从2.05×1014个·m L-1降低到1.06×106个·m L-1,发酵液的后处理达到了除菌和右旋糖酐高收率的目标。
引文
[1]NAESSENS M,CERDOBBEL A,SOETAERT W,et al.Review Leuconstoc dextransucrase and dextran:production,properties and appiications[J].Chem Technol Biot,2005,80:845-860.
[2]SHAMALA TR,PRASAD MS.Preliminary studies on Leuconostoe spp.[J].Process Biochem,1995,30(03):237-241.
[3]BHAVANI AL,NISHA J.Dextran-the poiysaccharide with versatile users[J].Int J Pharm Bio Sci,2010,4(01):569-573.
[4]慕娟,问清江,孙晓宇,等.右旋糖酐发酵条件的研究[J].陕西农业科学,2016,(12):33-36.
[5]杜连祥,路福平.微生物学实验技术[M].北京:中国轻工业出版社,2005:20-22.
[6]El-Sayed M M,Mahmoud W M,Coughlin.Production of dextransucrase by Leuconstoc mesenteriodes immobilized in calcium-alginate beeds:(1)Batch and fed-batch fermentation[J].Biotechnology and Bioengieering,1990,36(5):338-345.
