摘要
基于液晶取向变化构建液晶生物传感器用以检测人类β防御素-2(HBD-2)。传感器基底经过3-氨丙基三乙氧基硅烷(APTES)与N,N-二甲基十八烷基(3-(三甲氧基硅丙基))氯化铵(DMOAP)混合溶液组装氨基化膜,再利用戊二醛(GA)对组装膜进行醛基化修饰用以固定HBD-2抗体。当基底表面固定的HBD-2抗体与一定浓度范围内的HBD-2特异性结合后,改变了传感器基底形貌,扰乱了液晶分子的有序排列,从而引起光学信号的改变,可用于检测HBD-2。当HBD-2的含量高于5.0ng/mL时,可观测到明显的光学图像变化。本方法易操作,无需标记,灵敏度较高,特异性好,响应速度快。
A lable-free liquid crystal,4-biphenyl-4'-pentyl-carbonitril(5CB)biosensor based on the orientation changes of liquid crystal was developed for the detection of human beta 2(HBD-2)defensin through direct immunization assay.HBD-2was tethered onto the assembled film surface on a glass slide via a cross-linker glutaradehyde.The(3-aminopropyl)trimethoxysilane/N,N-dimethyl-N-octadecyl(3-aminopropyl)trimethoxysilyl chloride/glutaraldehyde(APTES/DMOAP/GA)mixed self-assembled monolayers formed by both long and short alkyls could cause uniform vertical alignment of liquid crystal.The specific binding of anti-HBD-2antibody and HBD-2induced the homeotropic-to-tilted transition of liquid crystal,making a visible optical change observed under the crossed polarized light and achieving the detection of HBD-2.When the concentration of HBD-2exceeded a critical value(5ng/mL),the opitical images showed a very strong response.This study offers a simple,highly sensitive and specific,lable-free method for HBD-2detection.
引文
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