摘要
目的探讨BDNF修饰的骨髓间充质干细胞(BMSCs)对缺氧缺血性脑损伤(HIBI)大鼠脑区BDNF-TrkB通路表达的影响与意义。方法将96只健康7日龄清洁级SD大鼠随机分为空白组、模型组、BMSCs干预组和BDNF-BMSCs干预组,每组各24只。模型组、BMSCs干预组和BDNF-BMSCs干预组采用颈动脉结扎和密封缺氧箱处理法制备HIBI模型,在此基础上,BMSCs干预组尾静脉注射BMSCs,BDNF-BMSCs干预组尾静脉注射BDNF基因修饰的BMSCs。采用Morris水迷宫实验观察大鼠逃避潜伏期和定位探索次数,采用多聚甲醛心脏灌注法取完整大脑,评估形态学评分,采用WB实验测定脑组织BDNF和TrkB的表达水平。结果与空白组相比,模型组、BMSCs干预组和BDNF-BMSCs干预组逃避潜伏期均延长,空间探索次数减少;与模型组相比,BMSCs干预组和BDNF-BMSCs干预组逃避潜伏期缩短,空间探索次数增多;与BMSCs干预组相比,BDNF-BMSCs干预组逃避潜伏期进一步缩短,空间探索次数进一步增多;组间差异均有统计学意义(均P<0. 05)。与空白组相比,模型组、BMSCs干预组和BDNF-BMSCs干预组形态学评分升高,BDNF和TrkB蛋白表达水平降低;与模型组相比,BMSCs干预组和BDNF-BMSCs干预组形态学评分降低,BDNF和TrkB蛋白表达水平升高;与BMSCs干预组相比,BDNF-BMSCs干预组形态学评分进一步降低,BDNF和TrkB蛋白表达水平进一步升高,组间差异均有统计学意义(均P<0. 05)。结论 BDNF修饰的BMSCs能够上调HIBI大鼠脑区BDNF-TrkB通路表达,从而发挥神经保护作用,促进疾病转归,具有较高的应用价值。
引文
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