摘要
目的建立HPLC法同时测定17种(含2待发表新种)石杉亚科植物中石杉碱甲(huperzine A)和石杉碱乙(huperzine B)的含有量。方法石杉碱甲、乙酒石酸提取液的分析采用Wondasil C_(18)色谱柱(4.6 mm×250 mm,5μm);以甲醇-醋酸铵溶液为流动相,等度洗脱;体积流量1.0 mL/min;检测波长310 nm;柱温室温。外标法定量。结果石杉碱甲、乙分别在0.002 0~0.30μg、0.001 8~0.27μg范围内线性关系良好(r=0.999 9),平均加样回收率、RSD分别为103.86%(1.85%)、101.3%(1.30%)。17种石杉亚科植物中都含有石杉碱甲、乙,各物种之间含有量有显著性差异;喜马拉雅马尾杉中石杉碱甲含有量最高,达0.22%,柳杉叶马尾杉、有柄马尾杉和马尾杉含量也较高;马尾杉中石杉碱乙含有量最高。结论该方法准确稳定,重复性好,可用于石杉亚科植物中石杉碱甲和石杉碱乙的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of huperzine A and huperzine B from seventeen species Huperzioideae subfamily( including 2 ineditus new species). METHODS The analysises of tartaricacid extracts of huperzine A and huperzine B were performed on a room temperature column( 4. 6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-spiritus mindereri flowing at 1. 0 mL/min in a isocratic elution manner,and the detection wavelength was set at 310 nm. The contents were determined by external standards. RESULTS Huperzine A and huperzine B showed good linear relationships within0. 002 0-0. 30 μg,0. 001 8-0. 27 μg( r = 0. 999 9),whose average recoveries were 103. 86%,101. 3% with the RSDs of 1. 85%,1. 30%,respectively. Huperzine A and huperzine B were found in seventeen species,and there were significant differences in contents from species to species. The content of huperzine A in Phlegmariurus hamiltonii was the highest,which reached up to 0. 22%,with higher levels of it in Phlegmariurus cryptomerianus,Phlegmariurus petiolatus,and Phlegmariurus phlegmaria; The content of huperzine B was the highest in P. phlegmaria. CONCLUSION This accurate,stable and reproducible method can be used for the quality control of huperzine A and B from Huperzioideae subfamily.
引文
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