摘要
目的:研究榄钱含片对东莨菪碱致小鼠学习记忆障碍的改善作用。方法:将小鼠随机分为空白组(生理盐水)、模型组(生理盐水)、阳性组(盐酸多奈哌齐片,1.52 mg/kg)和榄钱含片高、中、低剂量组(800、400、200 mg/kg),每组14只。每天1次,连续灌胃给药30 d后,除空白组外,其余各组小鼠腹腔注射氢溴酸东莨菪碱3 mg/kg制备记忆障碍模型。造模后,采用避暗实验(观察指标为潜伏期、进入暗室错误次数)和Morris水迷宫实验(观察指标为登台时间、穿台次数、目标象限游泳时间)评价各组小鼠学习记忆能力的变化,然后检测各组小鼠脑组织中乙酰胆碱(Ach)、乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(AchE)、超氧化物歧化酶(SOD)、丙二醛(MDA)的水平。结果:与空白组比较,模型组小鼠潜伏期、穿台次数、目标象限游泳时间及ChAT、Ach、SOD水平显著缩短或降低(P<0.05或P<0.01),进入暗室错误次数、登台时间及AchE、MDA水平显著延长或升高(P<0.01);与模型组比较,阳性组和榄钱含片各剂量组小鼠潜伏期(除榄钱含片低剂量组)、穿台次数、目标象限游泳时间及ChAT、Ach、SOD水平显著延长或升高(P<0.05或P<0.01),进入暗室错误次数(除榄钱含片低剂量组)、登台时间(除榄钱含片低剂量组)及AchE、MDA(除榄钱含片中、低剂量组)水平显著缩短或降低(P<0.05或P<0.01)。结论:榄钱含片可能通过增加ChAT、Ach、SOD水平及降低AchE、MDA水平改善东莨菪碱致小鼠学习记忆障碍。
OBJECTIVE:To study the improvement effects of Lanqian buccal tablets on scopolamine-induced learning and memory impairment of mice. METHODS:The mice were randomly divided into blank group(normal saline),model group(normal saline), positive group(Donepezil hydrochloride tablets, 1.52 mg/kg) and Lanqian buccal tablets high-dose,medium-dose and low-dose groups(800,400,200 mg/kg),with 14 mice in each group. Once a day,30 days after continuous intragastric administration,except for blank group,other groups were intraperitioneal injected scopolamine hydrobromide 3 mg/kg to induce learning and memory impairment model. After modeling,the learning and memory ability of mice were evaluated with step through test(latency,mistake times of entering darkroom as indexes)and Morris water maze(the time of going up on the platform,the times of crossing the platform,swimming time in target quadrant as indexes). The levels of Ach,ChAT,AchE,SOD and MDA in cerebral tissue of mice were determined. RESULTS:Compared with blank group,latency,the times of crossing the platform,swimming time in target quadrant,the levels of ChAT,Ach and SOD were shortened or decreased significantly in model group(P<0.05 or P<0.01),while mistake times of entering darkroom,the time of going up on the platform,the levels of AchE and MDA were extended or rised significantly(P<0.01). Compared with model group,latency(except for Lanqian buccal tablet low-dose group),the times of crossing the platform,swimming time in target quadrant,the levels of ChAT,Ach and SOD were extended or rised significantly in positive group and Lanqian buccal tablet groups(P<0.05 or P<0.01). The mistake times of entering darkroom(except for Lanqian buccal tablet low-dose group),the time of going up on the platform(except for Lanqian buccal tablet low-dose group) and the levels of AchE and MDA(except for Lanqian buccal tablet medium-dose and low-dose group) were shortened or decreased significantly(P<0.05 or P<0.01). CONCLUSIONS:Lanqian buccal tablet can improve scopolamine-induced learning and memory impairment of mice by increasing the levels of ChAT, Ach and SOD and decreasing the levels of AchE and MDA.
引文
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