摘要
目的 :探讨脱细胞支架(AS)联合电针对坐骨神经损伤(SNI)大鼠脊髓前角运动神经元的保护作用。方法 :首先制备AS,用于桥接损伤的神经。其次切除大鼠右侧坐骨神经10 mm,建立大鼠SNI模型。将SNI模型大鼠随机分为模型组(M)、AS桥接组(AS)和AS联合电针治疗组(AST)。模型组不予任何干预,AS组将支架桥接于两断端处,AST组在支架桥接术后2 d给予电针进行治疗,采用20 Hz、1 mA疏密波相间的电流,针刺穴位为环跳和阳陵泉,每次电针15 min,7 d 1个疗程。电针4周后,用电生理记录仪检测各组大鼠坐骨神经传导速度和波幅,用尼氏染色观察各组大鼠脊髓前角运动神经元的形态结构,用免疫印迹检测各组大鼠脊髓脑源性神经营养因子(BDNF)和神经生长因子(NGF)蛋白的表达。结果 :AST组大鼠坐骨神经传导速度和波幅明显高于AS组;尼氏染色显示AST组脊髓前角运动神经元胞体形态较完整,尼氏体呈蓝紫色、斑块状,偶见部分核移位现象,尼氏体的数量明显多于AS组和模型组;免疫印迹结果显示AST组脊髓内BDNF和NGF蛋白表达量均高于AS组和模型组。结论 :脱细胞支架联合电针不仅可增加大鼠坐骨神经传导速度及波幅,还可阻止脊髓前角运动神经元中尼氏体肿胀与溶解,并可上调脊髓内BDNF和NGF蛋白的表达,对SNI所致的脊髓前角运动神经元损伤有保护作用。
Objective : To investigate the protective effect of acellular scaffolds(AS) combined with electroacupuncture(EA) on the motor neurons in anterior horn of spinal cord in rats with sciatic nerve injury(SNI). Methods : Firstly AS were prepared for bridging injured nerves. Then the right sciatic nerves were excised 10 mm and the SNI model was established. The rats of the SNI model were randomly divided into model group(M),AS bridge group(AS),and AS combined with EA treatment group(AST). The rats in the model group received no intervention after SNI. The rats in the AS group were bridged with AS,and the rats in the AST group were treated with EA 2 days after bridging surgery,and then the current with rarefaction and dense waves was adopted by 20 Hz,1 mA. Huantiao and Yanglingquan were selected as the acupuncture points. Each EA lasted 15 minutes and 7 days as a course of treatment. 4 weeks after EA,sciatic nerve conduction velocity and amplitude were measured by electrophysiological recorder. Morphological structure of motor neurons in anterior horn of spinal cord was observed by Nissl staining. The expression of brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) were detected by Western blotting. Results : The conduction velocity and amplitude of sciatic nerve in AST group were significantly higher than that in AS group. Nissl staining showed that neuronal soma of the anterior horn motor neurons in AST group was intact. Nissl bodies were blue and purple plaques. The number of Nissl bodies was higher than that in AS and M groups. The results of Western blotting showed that the expressions of BDNF and NGF protein in AST group were higher than those in AS and M groups. Conclusion : AS combined with EA not only increases the conduction velocity and amplitude of sciatic nerve in rats,but also prevents the swelling and dissolution of Nissl bodies in anterior horn motor neurons in the spinal cord,upregulates the expression of BDNF and NGF protein in the spinal cord,so they have protective effect on the motor neurons in anterior horn of the spinal cord in rats with SNI.
引文
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