摘要
目前常用的外源基因表达系统主要分为两类:原核表达系统和真核表达系统。前者主要包括大肠杆菌表达系统和枯草杆菌表达系统,后者一般包括酵母表达系统、昆虫/杆状病毒表达系统、哺乳动物细胞表达系统以及新兴的转基因植物表达系统等。在这众多的表达系统中酵母表达系统因其培养比较方便、简单、价格低廉、外源蛋白表达量高、表达的蛋白活性好、能进行翻译后修饰等诸多优点脱颖而出,成为目前最主要的外源基因表达系统。概述了常见的几种酵母表达系统的特点,并对发酵过程中影响外源蛋白表达量的因素做了探讨,以期为人们表达重组蛋白时选择合适的表达系统和获得高效的表达产物提供帮助。
Nowadays,the commonly used exogenous gene expression system mainly includes prokaryotic expression system and eukaryotic expression system. In which the former mainly includes E. coli expression system and Bacillus subtilis expression system,while the latter includes yeast expression system,insect/baculovirus expression system,mammalian cell expression system and emerging transgenic plant expression system. In this large number of expression systems,yeast expression system becomes the most important exogenous gene expression system,which stands out from other expression systems because of its various advantages,such as convenience,simpleness,price moderate,high expression of exogenous protein,high quality of expressed protein,the modification of explanation and so on. This paper summarizes the characteristics of several common yeast expression systems,as well as the factors influencing the expression of exogenous proteins in the fermentation process aiming at offering helps in choosing a suitable expression system and obtaining efficient expression products when people express recombinant protein.
引文
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