摘要
随着糖蛋白类药物的需求量不断增加,酵母表达系统的人源化改造成为当务之急。其中,酵母蛋白的O-糖基化因O-糖链种类繁多、组分单一以及糖基化位点预测困难等因素,限制了酵母蛋白的O-糖基化人源化改造。从酵母蛋白的O-糖链结构、O-糖链发生过程及O-糖链的功能展开综述,重点介绍起始酵母蛋白O-糖基化过程的O-甘露糖转移酶家族(family of protein O-mannosyltransferases, PMTs)成员的研究现状,希望对酵母蛋白O-糖基化人源化改造研究提供参考。
With the growing global demand for glycoprotein biopharmaceuticals, the humanization of yeast expression system becomes imperative. However, the varieties of O-glycans, simple composition and unpredictable glycosylation sites have hampered the humanization of yeast O-glycosylation. This review summarized the structures, biosynthesis pathway and biological functions of O-glycans from yeast proteins and introduced O-mannosy-ltransferase family(PMTs) which catalyzed initial biosynthesis pathway of O-glycosylation,intending to provide a reference to the studies on humanization of yeast O-glycosylation.
引文
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