摘要
肠膜状明串珠菌在其产生的右旋糖酐蔗糖酶的作用下,以蔗糖为原料转化合成右旋糖酐和产生果糖。着重进行了Mn~(2+)对肠膜状明串珠菌Lm-1226发酵产右旋糖酐影响的初步探索。对Mn~(2+)对肠膜状明串珠菌Lm-1226的生长,产果糖、右旋糖酐和右旋糖酐蔗糖酶,右旋糖酐蔗糖酶作用影响进行了研究。一定浓度的Mn~(2+)对肠膜状明串珠菌Lm-1226的生长具有促进作用; Mn~(2+)抑制肠膜状明串珠菌Lm-1226发酵产果糖和右旋糖酐,且Mn~(2+)浓度越高,抑制性越强; Mn~(2+)对肠膜状明串珠菌Lm-1226发酵产右旋糖酐蔗糖酶有抑制作用; Mn~(2+)对右旋糖酐蔗糖酶具有较强的激活作用,激活作用可达158%,最适Mn~(2+)浓度为5. 0 mmol/L。
Affected by dextransucrase produced by Leuconstoc mesenteriodes inverted sucrose as raw material into dextran and produced fructose. This research focuses on the effect of Mn~(2+)on L. mesenteriodes and initial probe the effects on the fermentation and the production of dextran. And carry out the study on the effect of Mn~(2+)on L. mesenteriodes Lm-1226 growth,fructose,and dextran production,as well as the effect on dextransucrase reaction. A certain concentration of Mn~(2+)could enhance the growth of L. mesenteriodes Lm-1226: Mn~(2+)inhibited L. mesenteriodes Lm-1226 fermentation to produce fructose and dextran,and this inhibitory effect was stronger as Mn~(2+)concentration higher. Mn~(2+)inhibited L.mesenteriodes Lm-1226 to produce dextransucrase. However,Mn~(2+)possessed fairly strong dextransucrase activation,and this activation effect could reach up to 158%. The optimal concentration of Mn~(2+)was at 5. 0 mmol/L.
引文
[1] Monchois V,Willemot RM,Monsa P. Glucansucrase:mechanism of action and structure-relationships[J]. FEMS Miicrobiol Lett,1999,23:131-151.
[2] Davies G,Enrissat B. Structures and mechanisms of glycosyl hydrolases[J]. Structure,1995,3:853-859.
[3] Santos M,Teixeire J,Rodrigues A. Production of dextransucrase,dextran and fructose using Leuconstoc mesenteriodes NRRLB512[J]. Biochemical Engineering Journal,2000,4:177-188.
[4] Kim D,Robyt JF,Lee SY,et al. Dextran molecular size and degree of branching as a function of sucrose concentration,pH,and temperature of Leuconstoc mesenteriodes B512FMCM dextransucrase[J]. Carbohydrate Research,2003,338(1):1183-1189.
[5] Berensmeier S,Ergezinger M,Bohnet M,et al. Design of immobilized dextransucrase of fluidized bed application[J]. J Biotechnol,2004,114:255-267.
[6] El-Sayed MM,Mahmoud WM,Coughlin. Production of dextransucrase by Leuconstoc mesenteriodes immobilized in calciumalginate beeds:(1)Batch and fed-batch fermentation[J]. Biotechnology and Bioengieering,1990,36(5):338-345.
[7]李清娣,高歌.右旋糖酐工艺与影响质量的因素[J].中国医学工业杂志,1994,25(2):91-92.
[8]李良铸,李明晔.最新生化药物制备技术[M].北京:中国医药科技出版社,2003:229-301.
[9]中华人民共和国药典委员会.中华人民共和国药典(2000版)(二部)[M].北京:化学工业出版社,2000:111-115.
[10]花逾冬,曾和.膜分离制备右旋糖酐[J].中国医药工业杂志,2008,31(10):738-740.
[11]张洪斌,王雅洁,胡雪芹,等.右旋糖酐蔗糖酶生产菌的产酶条件研究[J].食品科学,2008,29(5):303-306.
[12]李攀峰,张洪斌,胡雪芹. IPTG与乳糖联合诱导重组大肠杆菌右旋糖酐蔗糖酶表达[J].食品科学,2014,35(5):185-188.
[13]吴兆鹏,曾练强,蚁细苗,等.蔗糖发酵右旋糖酐工艺条件的优化[J].广西蔗糖,2012,67(2):39-43.
[14]邹青松,浦媛媛,王晓,等.酶法合成右旋糖酐及过程调控研究进展[J].食品与发酵工业,2011,37(12):100-104.
[15]王雅洁,张洪斌,胡雪芹,等.重组大肠杆菌右旋糖酐蔗糖酶的纯化及其性质[J].微生物学报,2008,48(9):1266-1269.
