摘要
目的:通过动物实验观察针刺对神经源性肌萎缩的治疗作用,探讨其作用机制。方法:双重结扎大鼠坐骨神经复制神经源性肌萎缩动物模型,针刺环跳、阳陵泉、足三里等穴位,观察腓肠肌湿重(脏器指数)及病理改变,检测肌组织的钠钾ATP酶、NT-3、IGF-Ι、NGF含量。结果:双重结扎大鼠坐骨神经可复制神经源性肌萎缩动物模型,针刺环跳、阳陵泉、足三里等穴位可减轻萎缩肌组织的病理改变,增加萎缩肌组织的湿重和脏器指数,提高钠钾ATP酶活性和NT-3、NGF活性。结论:针刺可治疗双重结扎大鼠坐骨神经引起的神经源性肌萎缩,其机制与提高钠钾ATP酶活性和NT-3、NGF含量密切相关。
Objective: To observe the effect of acupuncture on neurogenic muscular according to animal experiment. Methods: The animal model of neurogenic muscular was established by double ligation of rat sciatic nerve. The Huantiao( 环 跳), Yanglingquan( 阳陵泉) and Zusanli( 足三里) points of rats were given acupuncture, to observe the wet weight of gastrocnemius muscle(Viscera index)and pathological change, and detect Na+-K+-ATPase, NT-3, IGF-I and NGF in muscle tissue. Results: The sciatic nerve ligation can copy neurogenic muscular atrophy animal model; acupuncture in Huantiao, Yanglingquan, Zusanli and other points can reduce the pathological change of muscle atrophy, increase the wet weight and organ index of the atrophy muscle tissue, improve Na+-K+-ATPase, NT-3 and NGF activity. Conclusion: Acupuncture can treat neurogenic atrophy caused by sciatic nerve ligation in rats; its mechanism is closely related to the improvement of Na+-K+-ATPase, NT-3 and NGF activity.
引文
[1]Ma Yong-cheng,Hao Yong-qiang.Design and establishment of a ratmodel of experimental local muscular atrophy[J].Journal of ClinicalRehabilitative Tissue Engineering Research,2010,14(7):1223.
[2]Rantakokko J,Uusitalo H,Jms T,et al. Expression profiles of mRNAsfor osteoblast and osteoclast proteins as indicators of bone loss in mouseimmobilization osteopenia model[J]. J Bone Miner Res,1999,14(11):1934.
[3]Morey-Holton ER,Globus RK. Hindlimb unloading of growing rats:amodel for predicting skeletal changes during space flight[J].Bone,1998,22(5):83.
[4]Weinreb M,Rodan GA,Thompson DD. Depression of osteoblasticactivity in immobilized limbs of suckling rats[J]. J Bone Miner Res,1991,6(7):725.
[5]徐建广,顾玉东.大鼠失神经支配骨骼肌萎缩的实验研究[J].中华实验外科杂志,2004,21(1):57-58.
[6]车光昇,郭源秩,宋光熠.神经源性肌萎缩病理动物模型的制备[J].辽宁医学杂志,2014,28(5):封三.
[7]Borisov AB,S Huang&B.M.Carlson.Remodeling of the vascular bedand progressive loss of capillaries in denervated skeletal muscle[J].AnatRec,2000,258(3):292-304.
[8]徐建广,顾玉东.失神经支配骨骼肌退变组织形态学及电生理实验研究[J].中国修复重建外科杂志,1999,13(4):202-205.
[9]Bobinac D, Malnar-Drago Jevic D, Bajek S, et al, Muscle fiber typecomposition and morphometric properties of denervated ratextensordigitorum longus muscle[J].Croat Med J,2000,41(3):294-297.
[10]王中鹏,孙佳璐,孙忠人,等.针刺治疗失神经肌萎缩的机制及研究展望[J].针灸临床杂志,2012,28(9):73-75.
[11]Maisonpierre PC,Belluscio L,Squinto S, et al. Neurotrophin-3:aneurotrophic factor related to NGF and BDNF[J]. Science,1990,247(4949Pt1):1446-1451.
[12]Kamei N,Tanaka N,Oishi Y, et al. BDNF,NT-3,and NGF released fromtransplanted neural progenitor cells promote corticospinal axon growth inorganotypic cocultures[J]. Spine(Phila Pa1976),2007,32(12):1272-1278.
