摘要
目的通过比较三种常规动物麻醉药对C57BL/6小鼠心电图的影响,探寻对小鼠心电图干扰较小的麻醉药。方法将小鼠分为3组腹腔注射20%氨基甲酸乙酯,1%戊巴比妥钠,2. 5%水合氯醛,待完全麻醉后将小鼠连接上肢体导联的心电扫描仪,进行连续1 h的心电图记录。结果随给药时间延长(0～40 min),20%氨基甲酸乙酯对小鼠心电图各项指标无明显影响; 1%戊巴比妥钠可引起小鼠心率降低,PR间期,QRS波宽度明显延长;2. 5%水合氯醛使小鼠心率降低、PR间期延长,20 min后趋于稳定。结论 20%氨基甲酸乙酯对C57BL/6小鼠心电图各项指标影响最小。2. 5%水合氯醛在麻醉后20 min后适合进行相应科学研究。
Objective To investigate the anesthetics that do not interfere with electrocardiograms( ECG) by comparing the effects of three anesthetics on ECG in C57 BL/6 mice. Methods Mice were randomly divided into three groups: intraperitoneal injection of 20% ethyl carbamate,1% pentobarbital sodium,and 2. 5% chloral hydrate. After complete anesthesia,the mice were connected to an ECG scanner by a limb lead for 1 hour of electrocardiogram recording.Results Within the administration time between 0-40 min,20% EC did not significantly affect the ECG parameters of mice. However,mice in the 1% pentobarbital sodium group had a reduced heart rate,and prolonged PR interval and QRS duration. Mice in the 2. 5% chloral hydrate group had a reduced heart rate and prolonged PR interval. Conclusions Our findings indicates that 20% EC has the least effect on the ECG of C57 BL/6 mice and that 2. 5% chloral hydrate is suitable for experimental studies 20 min after anesthesia is induced in mice.
引文
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