摘要
为优化海藻膳食纤维的提取工艺,探究其对小鼠肠道菌的益生作用,采用《食品安全国家标准食品中膳食纤维的测定》(GB5009.88-2014),测得海带、裙带菜、海青菜、石花菜、龙须菜、坛紫菜、条斑紫菜七种海藻的总膳食纤维含量。结合pH变化探究其对有益菌的促生作用,筛得龙须菜为促进有益菌生长的最优海藻,其总膳食纤维含量为67.17%。采用单因素实验和响应面优化法,得到超声波提取龙须菜非可溶性膳食纤维(Insoluble Dietary Fiber,IDF)中残存的可溶性膳食纤维(Soluble Dietary Fiber,SDF)的最佳工艺条件:液料比(mL/g)95:1、时间50 min、温度50℃、功率200 W,在此提取工艺条件下龙须菜IDF中残留SDF的得率为8.23%。以龙须菜总膳食纤维(Total Dietary Fiber,TDF)、SDF为实验组,魔芋粉为阳性对照,探究其对抗生素造模肠道菌群失衡小鼠肠道菌群的恢复效果,短期研究发现实验组能够改善造模鼠肠道菌群丰度,利于有益菌增长和肠道环境恢复。研究结果填补了龙须菜膳食纤维(Dietary Fiber,DF)对肠道菌群益生作用研究的空白,可为海藻膳食纤维综合利用提供参考。
The purpose of this paper was to screen the optimal algal varieties using the yield of algal dietary fiber and the effect of beneficial bacteria as the evaluation index, and explore the effect of the algal dietary fiber on the intestinal flora of mice. The total dietary fiber(Total Dietary Fiber, TDF) content of seven kinds of marine algae, such as Laminaria japonica,Undaria pinnatiflda, Ulva lactuca, Gelidium amansii, Gracilaria lemaneiformis, Porphyra haitanensis, Porphyra yezoensis,was obtained using the national standard method(GB5009.88-2014). Combined with pH changes in mice enteric bacteria,Gracilaria lemaneiformis was selected as the optimal algal, and its total dietary fiber content was 67.17%. The soluble dietary fiber(SDF) in Gracilaria lemaneiformis was extracted by ultrasonic method using the response surface optimization based on the single factor experiment. The optimum ultrasonic conditions were shown as follows: the liqid-solid ratio of 95:1(mL/g), time 50 min, temperature 50 ℃, and power 200 W. Under the extraction conditions, the yield of residual SDF in G. lemaneiformis IDF was 8.23%. The recovery effect of G. lemaneiformis TDF and SDF to the imbalanced intestinal microflora mice induced by antibiotics was explored with Amorphophallus konjac powder used as the positive control. The results indicated that the abundance of intestinal flora was improved in the mice model in short time, which facilitates the growth of beneficial bacteria and restores the intestinal environment. The results of this experiment filled the blank of the study on the probiotic effects of DF of G. lemaneiformis on mice intestinal microflora, providing a reference for the comprehensive utilization of algal dietary fiber.
引文
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