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Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells
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- 英文篇名:Single-cell RNA sequencing profiling of the effects of aging on alveolar stem cells
- 作者:Tingting ; Lv ; Kewu ; Jiang ; Jiawen ; Wang ; Nan ; Tang ; Huaping ; Dai ; Chen ; Wang
- 英文作者:Tingting Lv;Kewu Jiang;Jiawen Wang;Nan Tang;Huaping Dai;Chen Wang;Department of Pulmonary and Critical Care Medicine,Center of Respiratory Medicine,China-Japan Friendship Hospital,National Clinical Research Center for Respiratory Diseases,Institute of Respiratory Medicine Chinese Academy of Medical Sciences;Graduate School of Peking Union Medical College,Chinese Academy of Medical Science and Peking Union Medical College;National Institute of Biological Sciences;
- 英文关键词:single-cell RNA sequencing (scRNA-seq);;aging;;pulmonary alveolar stem cells;;inflammation response;;lipid metabolism
- 中文刊名:JCXG
- 英文刊名:中国科学:生命科学(英文版)
- 机构:Department of Pulmonary and Critical Care Medicine,Center of Respiratory Medicine,China-Japan Friendship Hospital,National Clinical Research Center for Respiratory Diseases,Institute of Respiratory Medicine Chinese Academy of Medical Sciences;Graduate School of Peking Union Medical College,Chinese Academy of Medical Science and Peking Union Medical College;National Institute of Biological Sciences;
- 出版日期:2019-07-19 14:44
- 出版单位:Science China(Life Sciences)
- 年:2019
- 期:v.62
- 基金:supported by the National Natural Science Foundation of China (81430001 and 81870056);;
Beijing Major Science and Technology Projects (Z171100000417003 to N.T.)
- 语种:英文;
- 页:JCXG201908004
- 页数:10
- CN:08
- ISSN:11-5841/Q
- 分类号:40-49
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摘要
The aging of alveolar stem cells has been linked to many chronic lung diseases, including pulmonary fibrosis. However, the effects of aging on alveolar stem cells during homeostasis and post-injury alveolar repair have not been well characterized. Here we conducted a single-cell RNA sequencing(scRNA-seq) analysis of alveolar stem cells of 3-month-old and 12-month-old mice to characterize the aging effect on alveolar stem cells. Our results have shown that the transcriptomes of alveolar stem cells of 3-month-old and 12-month-old mice are not significantly different under the steady condition. However, after a bleomycin-induced lung injury, the alveolar stem cells of 12-month-old mice show enhanced inflammatory responses and decreased lipid metabolism. Our study suggests a close relationship among aging, lipid metabolism, inflammatory responses and chronic lung diseases. The aging of alveolar stem cells has been linked to many chronic lung diseases, including pulmonary fibrosis. However, the effects of aging on alveolar stem cells during homeostasis and post-injury alveolar repair have not been well characterized. Here we conducted a single-cell RNA sequencing(scRNA-seq) analysis of alveolar stem cells of 3-month-old and 12-month-old mice to characterize the aging effect on alveolar stem cells. Our results have shown that the transcriptomes of alveolar stem cells of 3-month-old and 12-month-old mice are not significantly different under the steady condition. However, after a bleomycin-induced lung injury, the alveolar stem cells of 12-month-old mice show enhanced inflammatory responses and decreased lipid metabolism. Our study suggests a close relationship among aging, lipid metabolism, inflammatory responses and chronic lung diseases.
引文
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