摘要
目的研究简易自发气调包装及不同浓度茉莉酸甲酯(MeJA)处理对采后蓝莓果实抗性的影响。方法分别用浓度为0.15,0.3 mmol/L的MeJA以及去离子水浸泡蓝莓果实,时间均为10 min,将取出的果实晾干、预冷,装入厚度为0.0294 mm的聚乙烯简易自发气调包装袋中,于1℃下贮藏,以15 d为1周期对蓝莓呼吸代谢及抗性相关指标进行测定,即袋内CO_2和O_2浓度,以及果实亮度、感官评定、呼吸速率、硬度、腐烂率、pH值、可溶性固形物(SSC)含量、苯丙氨酸解氨酶(PAL)活性、多酚氧化酶(PPO)活性、过氧化物酶(POD)活性、木质素含量等。结果 MeJA处理可有效保持包装袋内适宜的CO_2和O_2浓度,并可减缓果实硬度、亮度的下降,抑制果实呼吸速率、pH值及SSC含量的上升,提高果实木质素含量,降低果实腐烂率,显著提高PAL,PPO,POD等抗性相关酶活性(P<0.05)。结论简易自发气调包装结合MeJA处理可通过抑制呼吸代谢、提高苯丙烷代谢及抗氧化酶活性,从而诱导采后蓝莓果实抗性的产生,保持采后果实品质。在2种浓度的MeJA处理中,浓度为0.15 mmol/L的处理效果最好。
The work aims to study the effects of treatment by simple spontaneous modified atmosphere packaging(S-MAP) and methyl jasmonate(MeJA) of different concentrations on the postharvest blueberry fruit resistance. Blueberry fruit was immersed in 0.15 and 0.3 mmol/L MeJA and deionized water for 10 min, respectively; then, the fruit was air-dried, precooled, filled into polyethylene S-MAP with a thickness of 0.0294 mm and stored at 1 ℃. The indexes of respiratory metabolism and resistance of blueberry were determined in a period of 15 d, including the concentration of CO_2 and O_2 in the S-MAP, fruit brightness, sensory evaluation, respiration rate, firmness, decay rate, pH, soluble id content(SSC), phenylalanine ammonia-lyase(PAL) activity, polyphenol oxidase(PPO) activity, peroxidase(POD) activity and lignin content. MeJA treatment could effectively maintain the appropriate concentration of CO_2 and O_2 in the bag, slow down the decline of fruit firmness and brightness, inhibit the rise of respiration rate, p H value and SSC, increase the lignin content, reduce the decay rate, and significantly improve the activities(P<0.05) of enzymes related to the resistance, such as PAL, PPO and POD. S-MAP combined with MeJA treatments can induce the generation of the fruit resistance and maintain the quality of postharvest fruits by inhibiting the respiratory metabolism, and increasing the metabolism of phenylpropane and the activity of antioxidant enzyme. During the MeJA treatments of two concentrations, the treatment effect is the best when theh concentration is 0.15 mmol/L.
引文
[1]潘利华,王建飞,叶兴乾,等.蓝莓花青素的提取工艺及其免疫调节活性[J].食品科学,2014,35(2):81—86.PAN Li-hua,WANG Jian-fei,YE Xing-qian,et al.Optimization of Extraction Process and Evaluation of Immunomodulatory Activity of Anthocyanins from Blueberry[J].Food Science,2014,35(2):81—86.
[2]陈杭君,王翠红,郜海燕,等.不同包装方法对蓝莓采后贮藏品质和抗氧化活性的影响[J].中国农业科学,2013,46(6):1230—1236.CHEN Hang-jun,WANG Cui-hong,GAO Hai-yan,et al.Effect of Packaging on the Postharvest Quality and the Antioxidant Activity of Blueberry[J].Scientia Agricultura Sinica,2013,46(6):1230—1236.
[3]许晴晴,陈杭君,郜海燕,等.真空冷冻和热风干燥对蓝莓品质的影响[J].食品科学,2014,35(5):64—68.XU Qing-qing,CHEN Hang-jun,GAO Hai-yan,et al.Effects of Vacuum Freeze-drying and Hot-air Drying on the Quality of Blueberry Fruits[J].Food Science,2014,35(5):64—68.
[4]李金星,胡志和.蓝莓花青素的研究进展[J].核农学报,2013,27(6):817—822.LI Jin-xing,HU Zhi-he.Advances in Research on Anthocyanins of Blueberry[J].Journal of Nuclear Agricultural Sciences,2013,27(6):817—822.
[5]黄晓杰.采后处理对蓝莓果实衰老的作用及机理研究[D].沈阳:沈阳农业大学,2016.HUANG Xiao-jie.Effect and Mechanism of Postharvest Treatments on Senescence of Blueberry Fruits[D].Shenyang:Shenyang Agricultural University,2016.
[6]姜爱丽,孟宪军,胡文忠,等.高CO2冲击处理对采后蓝莓生理代谢及品质的影响[J].农业工程学报,2011,27(3):362—368.JIANG Ai-li,MENG Xian-jun,HU Wen-zhong,et al.Effects of High CO2 Shock Treatment on Physiological Metabolism and Quality of Postharvest Blueberry Fruits[J].Transactions of the CSAE,2011,27(3):362—368.
[7]RADZIEJEWSKA-KUBZDELA E.The Effect of Pretreatment and Modified Atmosphere Packaging on Bioactive Compound Content in Coleslaw Mix[J].LWTFood Science and Technology,2017,75:505—511.
[8]王璇.茉莉酸甲酯等三种处理对黄冠梨果皮褐变的抑制作用及其机制[D].泰安:山东农业大学,2016.WANG Xuan.Effect of Me JA etc.on Peel Browning Inhibition of Postharvest Huangguan Pear and the Mechanism[D].Tai'an:Shandong Agricultural University,2016.
[9]CHEONG J J,CHOI Y D.Methyl Jasmonate as a Vital Substance in Plants[J].Trends in Genetics,2003,19(7):409.
[10]胡文忠,姜爱丽,蔡慧,等.茉莉酸甲酯处理对软枣猕猴桃生理生化变化的影响[J].食品工业科技,2013(4):331—334.HU Wen-zhong,JIANG Ai-li,CAI Hui,et al.Effect of Me JA Treatments on Physiological and Biochemical Changes for Actinidia Arguta[J].Science and Technology of Food Industry,2013(4):331—334.
[11]马杰,胡文忠,毕阳,等.茉莉酸甲酯处理对鲜切莴苣和甘蓝苯丙烷代谢的影响[J].食品工业科技,2013,34(7):327—329.MA Jie,HU Wen-zhong,BI Yang,et al.Effect of Me JA Treatments on Benzene Propane Metabolism in Tissues of Fresh-cut Lettuce and Cabbage[J].Science and Technology of Food Industry,2013,34(7):327—329.
[12]YAO H,TIAN S.Effects of Pre-and Post-harvest Application of Salicylic Acid or Methyl Jasmonate on Inducing Disease Resistance of Sweet Cherry Fruit in Storage[J].Postharvest Biology&Technology,2005,35(3):253—262.
[13]YAO H J,TIAN S P.Effects of a Biocontrol Agent and Methyl Jasmonate on Postharvest Diseases of Peach Fruit and the Possible Mechanisms Involved[J].Journal of Applied Microbiology,2005,98(4):941—950.
[14]GONZáLEZ-AGUILAR G A,TIZNADO-HERNáNDEZ M E,ZAVALETA-GATICA R,et al.Methyl Jasmonate Treatments Reduce Chilling Injury and Activate the Defense Response of Guava Fruits[J].Biochemical&Biophysical Research Communications,2004,313(3):694—701.
[15]付亮,刘诗扬,徐方旭.茉莉酸甲酯对蓝莓果实生理生化变化的影响[J].沈阳师范大学学报(自然科学版),2017,35(4):485—487.FU Liang,LIU Shi-yang,XU Fang-xu.Effect of Treatment with Me JA on Physiology and Biochemistry in Blueberry Fruit[J].Journal of Shenyang Normal University(Natural Science Edition),2017,35(4):485—487.
[16]姜爱丽,胡文忠,孟宪军,等.外源水杨酸处理对采后蓝莓果实苯丙烷代谢的影响[J].食品工业科技,2013,34(6):334—337.JIANG Ai-li,HU Wen-zhong,MENG Xian-jun,et al.Effects of Exogenous Salicylic Acid Treatment on Phenylproanoid Metabolic System of Postharvest Blueberry Fruits[J].Science and Technology of Food Industry,2013,34(6):334—337.
[17]司琦,胡文忠,姜爱丽,等.动态气调贮藏对蓝莓采后生理代谢品质的影响[J].包装工程,2017,38(17):13—18.SI Qi,HU Wen-zhong,JIANG Ai-li,et al.Effects of Dynamic Modified Atmosphere Storage on Quality of Physiological Metabolism of the Postharvest Blueberry[J].Packaging Engineering,2017,38(17):13—18.
[18]姜爱丽,胡文忠,李慧,等.纳他霉素处理对采后甜樱桃生理代谢及品质的影响[J].农业工程学报,2009,25(12):351—356.JANG Ai-li,HU Wen-zhong,LI Hui,et al.Effect of Natamycin Treatment on Physiological Metabolism and Quality of Postharvest Sweet Cherry[J].Transactions of the CSAE,2009,25(12):351—356.
[19]曹建康,姜微波.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.CAO Jian-kang,JIANG Wei-bo.Experiment Guidance of Postharvest Physiolgy and Biochemistry of Fruits and Vegetables[M].Beijing:China Light Industry Press,2007.
[20]GONG K,CHEN L,LI X,et al.Lignin Accumulation and Biosynthetic Enzyme Activities in Relation to Postharvest Firmness of Fresh Waxy Corn[J].Journal of Food Processing&Preservation,2017(1):133.
[21]萨仁高娃,胡文忠,修志龙,等.活性壳寡糖涂膜处理对鲜切苹果品质的影响[J].包装工程,2017,38(17):25—30.SA Ren-gao-wa,HU Wen-zhong,XIU Zhi-long,et al.Effect of Active Chitosan Oligosaccharide Coating Treatment on Quality of Fresh-cut Apples[J].Packaging Engineering,2017,38(17):25—30.
[22]许晴晴,郜海燕,陈杭君.茉莉酸甲酯对蓝莓贮藏品质及抗病相关酶活性的影响[J].核农学报,2014,28(7):1226—1231.XU Qing-qing,GAO Hai-yan,CHEN Hang-jun.Effect of Me JA on Storage Quality and Disease-resistant Enzymes of Blueberries[J].Journal of Nuclear Agricultural Sciences,2014,28(7):1226—1231.
[23]GARCíA P C.Resistance to Cold and Heat Stress:Accumulation of Phenolic Compounds in Tomato and Watermelon Plants[J].Plant Science an International Journal of Experimental Plant Biology,2001,160(2):315.
[24]郑亚男,胡文忠,姜爱丽,等.茉莉酸甲酯对鲜切甘薯伤害防御反应的研究[J].食品工业科技,2012,32(2):368—372.ZHENG Ya-nan,HU Wen-zhong,JIANG Ai-li,et al.Research of Methyl Jasmonate on the Wounding Defense Response for Fresh-cut Sweet Potatoes[J].Science and Technology of Food Industry,2012(2):368—372.
[25]YAN J,SONG Y,LI J,et al.Forced-air Precooling Treatment Enhanced Antioxidant Capacities of Apricots[J].Journal of Food Processing&Preservation,2017(3):13320.
[26]WU Y,LIN H,LIN Y,et al.Effects of Biocontrol Bacteria Bacillus Amyloliquefaciens,LY-1 Culture Broth on Quality Attributes and Storability of Harvested Litchi Fruit[J].Postharvest Biology&Technology,2017,132:81—87.
[27]杨海燕,吴文龙,李维林,等.茉莉酸甲酯调控下采后蓝莓果实的生理响应研究[J].食品安全质量检测学报,2015(11):4483—4488.YANG Hai-yan,WU Wen-long,LI Wei-lin,et al.Effect of Methyle Jasmonate Treatments on Physioloical Changes of Blueberry Fruit[J].Journal of Food Safety and Quality,2015(11):4483—4488.
[28]弓德强,黄训才,黄光平,等.茉莉酸甲酯处理对采后芒果果实抗病性的影响[J].热带作物学报,2016,37(12):2294—2299.GONG De-qiang,HUANG Xun-cai,HUANG Guangping,et al.Effect of Methyl Jasmonate Treatment on Disease Resistance in Harvested Mango Fruit[J].Chinese Journal of Tropical Crops,2016,37(12):2294—2299.
[29]闫媛媛,胡文忠,姜爱丽,等.茉莉酸甲酯的信号分子作用及其在鲜切果蔬中应用的研究进展[J].食品工业科技,2015,36(2):384—387.YAN Yuan-yuan,HU Wen-zhong,JIANG Ai-li,et al.Research Progress on the Role of Methyl Jasmonate as Signal Molecules and Application in Fresh-cut Fruits and Vegetables[J].Science and Technology of Food Industry,2015,36(2):384—387.
[30]JIN P,WANG K,SHANG H,et al.Low-temperature Conditioning Combined with Methyl Jasmonate Treatment Reduces Chilling Injury of Peach Fruit[J].Journal of the Science of Food&Agriculture,2010,89(10):1690—1696.
[31]FAN L,SHI J,ZUO J,et al.Methyl Jasmonate Delays Postharvest Ripening and Senescence in the Non-climacteric Eggplant(Solanum Melongena,L.)Fruit[J].Postharvest Biology&Technology,2016,120:76—83.
[32]ZHU S,MA B.Benzothiadiazole-or Methyl Jasmonateinduced Resistance to Colletotrichum Musae in Harvested Banana Fruit is Related to Elevated Defense Enzyme Activities[J].Journal of Pomology&Horticultural Science,2015,82(4):500—506.
[33]WANG K,JIN P,HAN L,et al.Methyl Jasmonate Induces Resistance against Penicillium Citrinum,in Chinese Bayberry by Priming of Defense Responses[J].Postharvest Biology&Technology,2014,98(1):90—97.
[34]黄晓杰,李婧,柴媛,等.Me JA处理对蓝莓果实采后灰霉病的影响及机理[J].食品科学,2016,37(22):307—312.HUANG Xiao-jie,LI Jing,CHAI Yuan,et al.Effect and Mechanism of Methyl Jasmonate on Incidence of Grey Mould Decay in Postharvest Blueberry[J].Food Science,2016,37(22):307—312.
[35]秦国政,田世平,刘海波,等.拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导[J].中国农业科学,2003,36(1):89—93.QIN Guo-zheng,TIAN Shi-ping,LIU Hai-bo,et al.Po Iypheno I Oxidase,Peroxidase and Pheny Ia Ianine Ammonium Lyase in Postharvest Peach Fruits Induced by Inocu Iation with Pichia Membranefaciens or Rhizopus Stolonifer[J].Scientia Agricultura Sinica,2003,36(1):89—93.
