摘要
为了提高玉米蛋白的骨诱导性和骨结合性,以促进其在骨组织工程领域中的应用,将玉米蛋白多孔支架材料浸泡于5倍模拟体液(5×SBF)中,尝试在不同的条件下对玉米蛋白多孔支架材料表面进行仿生矿化。通过场发射扫描电镜(SEM)观察仿生矿化后多孔支架材料各个表面的形貌,能谱(EDS)计算出钙磷比(Ca/P)比。在5倍模拟体液中浸泡后,材料各表面均形成了分布和尺寸相对均匀的微米级(2~10 um)颗粒。其钙磷比接近羟基磷灰石,可以认为获得了比较理想的玉米蛋白-羟基磷灰石多孔支架复合材料。
In order to promote the application in bone tissue engineering,the zein scaffold was soaked in a solution of 5 times the concentration of simulated body fluid( 5 × SBF) to enhance its bone inductivity and osseointegration. The field emission scanning electron microscopy( SEM) was employed to observe the surface microstructures of the coating and energy dispersive spectrometer( EDS)which was attached to the SEM was used to carry out the elemental analysis and to calculate the ratio of calcium to phosphorus( Ca /P). The surface of the composite materials form uniform micro particles( 2- 10 um) that the ratio of calcium to phosphorus closed to that of hydroxyapatite( HA) after soaked in 5SBF. The zein- HA composite can be considered as an ideal material in bone tissue engineering.
引文
[1]Guo HX,Heinamaki J,Yliruusi J.Stable aqueous film coating dispersion of zein[J].J Colloid Interface Sci,2008,322(2):478-484.
[2]Lin T,Lu C,Zhu L,Lu T.The biodegradation of zein in vitro and in vivo and its application in implants[J].AAPS PharmSciTech,2011,12(1):172-176.
[3]Zhang M,Liu Y,Yi H,Luan J,et al.Electrospun zein/PVA fibrous mats as three-dimensional surface for embryonic stem cell culture[J].Journal of the Textile Institute,2014,105(3):246-255.
[4]Salerno A,Zeppetelli S,Oliviero M,et al.Microstructure,degradation and in vitro MG63 cells interactions of a new poly(-caprolactone),zein,and hydroxyapatite composite for bone tissue engineering[J].Journal of Bioactive and Compatible Polymers,2012,27(3):210-226.
[5]Qu ZH,Wang HJ,Tang TT,et al.Evaluation of the zein/inorganics composite on biocompatibility and osteoblastic differentiation[J].Acta Biomater,2008,4(5):1360-1368.
[6]Dong J,Sun Q,Wang JY.Basic study of corn protein,zein,as a biomaterial in tissue engineering,surface morphology and biocompatibility[J].Biomaterials,2004,25(19):4691-4697.
[7]Liu X,Sun Q,Wang H,,et al.Microspheres of corn protein,zein,for an ivermectin drug delivery system[J].Biomaterials,2005,26(1):109-115.
[8]Wang HJ,Lin ZX,Liu XM,et al.Heparin-loaded zein microsphere film and hemocompatibility[J].J Control Release,2005,105(1-2):120-131.
[9]Shields CL,Uysal Y,Marr BP,et al.Experience with the polymer-coated hydroxyapatite implant after enucleation in 126 patients[J].Ophthalmology,2007,114(2):367-373.
[10]Bharati S,Sinha M K,Basu D.Hydroxyapatite coating by biomimetic method on titanium alloy usingconcentrated SBF[J].Bulletin of Materials Science,2005,26(6):617-621.
[11]Sun T,Wang M.Low-temperature biomimetic formation of apatite/TiO2 composite coatings on Ti and NiTi shape memory alloy and their characterization[J].Applied Surface Science,2008,255(2):396-400.
[12]Wang CX,Chen Z Q,Wang M.Functionally graded calciumphosphate coatings produced by ion beamsputtering/mixing deposition[J].Biomaterials,2001,22(12):1619-26.
[13]Uchida M,Kim HM,Kokubo T,et al.Apatite-forming ability of a zirconia/alumina nano-composite induced by chemical treatment[J].J Biomed Mater Res,2002,60(2):277-282.
[14]Yoon JJ,Park TG.Degradation behaviors of biodegradable macroporous scaffolds prepared by gas foaming of effervescent salts[J].J Biomed Mater Res,2001,55(3):401-8.
[15]Chen Y,Mak AF,Li J,Wang M,et al.Formation of apatite on poly(alpha-hydroxy acid)in an accelerated biomimetic process[J].J Biomed Mater Res B Appl Biomater,2005,73(1):68-76.
[16]Kokubo T,Takadama H.How useful is SBF in predicting in vivo bone bioactivity[J].Biomaterials,2006,27(15):2907-2915.
[17]Hata K,Kokubo T,Nakamura T,Yamamuro T.Growth of a bonelike apatite layer on a substrate by a biomimetic process[J].Journal of the American Ceramic Society,1995,78(4):1049-1053.
