摘要
通过发酵实验比较分离得到的5株酵母菌株产尿素与高级醇的能力,筛选出低产尿素和高级醇的菌株AW001和J-16-2,并利用实时定量聚合酶链式反应技术验证,5株酵母菌的尿素产量与精氨酸吸收有关的CAR2基因拷贝数呈正相关。通过生理生化以及rDNA ITS序列的分析,鉴定其均为酿酒酵母。结合实验室模拟黄酒发酵实验的理化指标检测结果表明,菌株AW001、J-16-2发酵所得样品中总糖、总酸等含量均满足国标中黄酒发酵指标。因此,该2株菌为特性优良的酿酒酵母,有进一步开发利用的价值。
Five yeast isolates were compared for their capacities of producing urea and fusel alcohol. Strains AW001 and J-16-2 were selected for reduced production of urea and fusel alcohol. Urea production was positively correlated to the copy number of the CAR2 gene related to arginine assimilation as determined by real-time quantitative PCR. All strains were identified as Saccharomyces cerevisiae based on physiological and biochemical characteristics and rDNA ITS sequence analysis. Total sugar and total acid contents in the Chinese rice wines fermented with AW001 and J-16-2 met the national standard. These strains have great potential for development and utilization.
引文
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