摘要
<正>目前认为,腰椎间盘退变的发生机制有蛋白多糖的减少,髓核含水量的下降,炎性介质,椎间盘营养供应减少等。从分子生物学的角度来讲,在椎间盘的退变中多种信号通路发挥着一定的作用,如环磷酸腺苷-蛋白激酶A(Cyclic Adenosine monophosphate-protein kinase A,cAMP-PKA)信号通路、Wnt/β-catenin信号通路、NF-κB信号通路~([1])、MAPK通路等~([2])。现
引文
[1]孔敬波,马信龙,王涛,等.Wnt/β-catenin及NF-кB信号通路与椎间盘退变的研究进展[J].中国修复重建外科杂志.2013,27(12):1523-1528.
[2]刘玉亮,殷明,梁广胜,等.NF-κB信号和MAPK通路在椎间盘退变中的研究进展[J].生命科学,2012,24(7):674-679.
[3]宣雅波,杨肖敏,刘存志,等.针刺对多发梗塞性痴呆大鼠海马cAMP和cGMP含量的影响[J].中华中医药杂志,2012,27(2):352-355.
[4]韩曙光,赵弘卿.Epac:新的支气管哮喘治疗靶分子[J].中华哮喘杂志(电子版),2013,7(6):448-451.
[5]张威林,王海强,陈宇飞,等.椎间盘退变的分子机制研究现状[J].脊柱外科杂志,2012,10(4):253-256.
[6]Nerlich AG,Schaaf R,Walchli B,et al.Temporo-spatial distribution of blood vessels in human lumbar intervertebral discs[J].Eur Spine J,2007,16(4):547-555.
[7]秦超,史晨辉,刘维钢,等.椎间盘退变基础研究的历史回顾及展望[J].农垦医学,2009,31(6):552-555.
[8]Molinos M,Almeida CR,Caldeira J,etal.Inflammation in intervertebral disc degeneration and regeneration[J].J R Soc Interface,2015,12(104):1191-1197.
[9]Zhao CQ,Zhang YH,Jiang SD,et al.ADAMTS-5and intervertebral disc degeneration:the results of tissue immunohistochemistry and in vitro cell culture[J].J Orthop Res,2011,5(5):718-725.
[10]Yurube T,Takada T,Suzuki T.Rat tail static compression modelmimics extracellular matrix metabolic imbalances of matrix metalloproteinases,aggrecanases,and tissue inhibitors of metalloproteinases in intervertebral disc degeneration[J].Arthritis Res Ther,2012,2(1):51-59.
[11]杜伟,申勇,李宝俊.细胞因子IL-1a、IL-6、TNF-a、MMP3与颈椎间盘退变机制的相关性研究[J].中国矫形外科杂志,2012,20(3):259-262.
[12]王海莹,丁文元.肿瘤坏死因子-α在椎间盘细胞凋亡中的作用[J].脊柱外科杂志,2015,13(1):56-59.
[13]Ohba T,Haro H,Ando T,et al.TNF-alpha-induced NF-kap-paB signaling reverses age-related declines in VEGF inductionand angiogenic activity in intervertebral tissues[J].J OrthopRes,2009,27(2):229-235.
[14]刘健,于布为.调控环磷腺苷信号通路治疗病理性疼痛[J].中国药理学通报,2009,25(9):1134-1137.
[15]黄帅帅,王宇多,王萍.细胞骨架和cAMP/PKA信号通路的互动效应调控细胞行为的研究进展[J].Chinese Journal of Cell Biology,2014,36(7):1018-1026.
[16]赵霞,刘全宏,王筱冰.细胞骨架纤维间的相互联系[J].Chinese Journal of Cell Biology,2008,30(2):191-195.
[17]吴波,高清,刘琦,等.细胞凋亡过程中细胞骨架改变的激光共聚焦显微镜观察[J].电子显微学报,2010,29(4):361-366.
[18]曾顺福,刘杰,王建.兔退变椎间盘中髓核细胞凋亡与BNIP3基因表达的意义[J].中国修复重建外科杂志,2010,24(11):1367-1371.
[19]刘健,谢军明,嵇晴,等.环磷腺苷通路对内毒素刺激的小胶质细胞分泌细胞因子的影响[J].Chinese Pharmacological Bulletin,2012,28(4):478-482.
[20]李姣,王永华,黄芳,等.环磷腺苷注射液对大鼠脑缺血再灌注损伤的保护作用[J].实用医学杂志,2009,25(21):3583-3585.
[21]高维娟,许顺江,丛斌,等.CCK-8抗炎作用中DAG-PKC信号通路对cAMP-PKA信号通路的影响[J].Chinese Pharmacological Bulletin,2008,24(9):1156-1160.
[22]高维娟,许顺江,丛斌,等.CCK-8对大鼠肺间质巨噬细胞cAMPPKA信号通路的激活作用[J].Chinese Pharmacological Bulletin,2007,23(3):321-326.
[23]胡国超,舒钧,浦波.NF-κB信号转导通路及其与椎间盘退变的相关性研究进展[J].医学综述,2012,18(4):509-512.
[24]Scheidereit C.IκB kinase complexes:gateways to NF-κB activation and transcription[J].Oncogene,2006,51(10):6685-6705.
[25]Bezbradica JS,Medzhitov R.Integration of cytokine and heterologousreceptor signaling pathways[J].Nat Immunol,2009,4(5):333-339.
[26]Nasto LA,Seo HY,Robinson AR,et al.ISSLS prize winner:inhibi-tion of NF-κB activity ameliorates age-associated disc degeneration in a mouse model of accelerated aging[J].Spine,2012,21(11):1819-1825.
[27]范琳琳,夏仲年,杨成志,等.力竭游泳运动后大鼠伏核内NF-κB、cAMP的表达[J].Chinese Journal of Neuroanatomy,2011,27(1):72-76.
[28]陈敏,余江平,黄敏.环核苷酸的生理作用及临床应用[J].中国药房,2007,18(11):872-874.
[29]王顺,张静.不同经穴针刺对脑卒中偏瘫痉挛大鼠血清cAMP、cGMP含量影响的研究[J].中国中医药科技,2012,19(1):54-55.
[30]Chen WH,Zeng R,Lo WC et al.The role of the ERK1/2pathway as an alternative to the aging-diminished cyclic AMP pathway in calcitonin-mediated chondrogenesis in human nucleus pulposus[J].Biomaterials,2012,33(33):8256-8264.
[31]Zhang L,Zambon AC,Vranizan K,etal.Gene expression signatures of cAMP/protein kinase A(PKA)-promoted,mitochondrial-dependent apoptosis:comparative analysis of wild-type and cAMP-deathless S49lymphoma cells[J].J Biol Chem.2008,283(7):4304-4313.
[32]房红芸,蒋与刚,刘静,等.缺锌对大鼠海马和皮层cAMP/PKACREB-BDNF信号转导通路的影响[J].营养学报,2008,30(2):153-156.
[33]漆洪波,路炜,段赵宁.调控人羊膜上皮细胞AQP8表达的cAMPPKA信号转导通路研究[J].现代妇产科进展,2010,19(2):81-86.
[34]文漫红.针灸对单纯性肥胖患者血浆环核苷酸含量的影响[J].中医药导报,2007,13(10):50-51.
