慢性炎性痛电针镇痛频率优选及其DRG TRPV1活化机制研究

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英文篇名：Selection of Optimal Analgesic Frequency on Electroacupuncture and Exploration of the Mechanism of DRG TRPV1 Activation for Chronic Inflammatory Pain 作者：项璇儿 ; 邵芳冰 ; 许颖龄 ; 杜俊英 ; 房军帆 ; 方剑乔 英文作者：Xiang Xuan'er;Shao Fangbing;Xu Yingling;Du Junying;Fang Junfan;Fang Jianqiao;The Third Clinical Medical College,Zhejiang Chinese Medical University Key Laboratory of Acupuncture and Neurology of Zhejiang Province; 关键词：电针 ; 频率 ; 优选 ; 炎性痛 ; 镇痛 ; DRG ; TRPV1 ; 活化 英文关键词：Electroacupuncture;;Frequency;;Optimal;;Inflammatory pain;;Analgesia;;DRG;;TRPV1;;Activation 中文刊名：SJZA 英文刊名：World Chinese Medicine 机构：浙江中医药大学第三临床医学院针灸神经生物学实验室浙江省针灸神经病学研究重点实验室; 出版日期：2019-07-09 11:05 出版单位：世界中医药 年：2019 期：v.14 基金：国家自然科学基金项目(81473772,81603690,81873360);; 浙江省医药卫生重大科技计划项目(WKJ-ZJ-1419) 语种：中文; 页：SJZA201906002 页数：6 CN：06 ISSN：11-5529/R 分类号：22-26+35

摘要

目的:筛选电针干预慢性炎性痛的优势频率,观察不同频率电针对慢性炎性痛大鼠DRG p-TRPV1的干预情况,探讨优势频率电针的DRG p-TRPV1活化干预机制。方法:所有实验大鼠完全随机分为空白组、模型组、2 Hz电针组、100 Hz电针组和2/100 Hz电针组。除空白组外,其余各组均进行CFA造模。电针组选用双侧"足三里"和"昆仑"穴,0. 5 m A～1. 5 m A,30 min/次。采用Von Frey Hair检测各组大鼠不同时间点患侧PWT和PWL;采用免疫印迹法检测大鼠DRG pTRPV1的表达。结果:100 Hz电针对CFA大鼠的PWT提升最为显著(P <0. 05),100 Hz、2/100 Hz电针均能有效提高CFA大鼠的PWL(P <0. 05,P <0. 05); CFA模型大鼠L4-6 DRG p-TRPV1蛋白表达均有上调,其中L4水平上调差异无统计学意义(P> 0. 05),L5、L6水平上升较为显著(P <0. 05),100 Hz电针能有效抑制CFA大鼠L5 DRG p-TRPV1蛋白的过表达(P <0. 05),对L4、L6 DRG p-TRPV1蛋白的过表达虽有下调趋势,但差异无统计学意义(P> 0. 05)。结论:100 Hz电针对慢性炎性痛的镇痛效应最佳,这可能与其能有效下调CFA大鼠L5 DRG p-TRPV1蛋白的过表达有关。
        Objective: To select the dominant frequency of electroacupuncture( EA) in treating chronic inflammatory pain,to observe the effects of different frequency EA on DRG p-TRPV1 of rats with chronic inflammatory pain,and to explore the mechanism of DRG p-TRPV1 activation by dominant frequency EA. Methods: All rats were randomly divided into a control group,a model group,a 2 Hz EA group,a 100 Hz EA group and a 2/100 Hz EA group. Except for the control group,rats of other groups were given CFA hypodermic injection into the plantar surface of the right hind paw of SD rats. In the EA group,Zusanli( ST36) and Kunlun( BL60) acupoints on both sides were selected,0. 5 m A-1. 5 m A,30 mins per time. Von Frey Hair was used to detect the PWT and PWL in the affected side of rats at different time points,and western blot was used to detect the expression of DRG p-TRPV1 in rats. Results: 100 Hz EA can effectively improve the PWT of CFA rats( P < 0. 05),and 100 Hz and 2/100 Hz EA can increase the PWL of CFA rats( P < 0. 05,P < 0. 05); the expression of L4-6 DRG p-TRPV1 protein in CFA model rats was up-regulated,and there is no significant difference in L4 level( P > 0. 05),otherwise L5 and L6 levels were up-regulated significantly( P < 0. 05). 100 Hz EA can effectively inhibit the overexpression of L5 DRG p-TRPV1 protein in CFA rats( P < 0. 05). While the overexpression of L4 and L6 DRG p-TRPV1 protein decreased,but there was no significant difference( P > 0. 05). Conclusion: 100 Hz EA has the best analgesic effect on chronic inflammatory pain,which may be related to its effective down-regulation of L5 DRG p-TRPV1 protein expression in CFA rats.

引文

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