摘要
目的了解西藏口岸蝇类携带病原体情况,为口岸卫生检疫执法工作提供技术支持。方法对2017年5-10月在西藏自治区拉萨贡嘎国际机场口岸、吉隆口岸、亚东口岸采集到的蝇类进行病原体检测,利用微生物分离纯培养技术及细菌16S rRNA基因序列鉴定和生理生化鉴定技术对蝇类样本进行细菌学检测,利用RT-PCR技术检测蝇类携带病毒情况。结果从蝇类样本中分离出粪肠球菌、蜂房哈夫尼亚菌、威斯康星米勒菌、大肠埃希菌、普通变形杆菌、耐久肠球菌等37种致病菌(条件致病菌)。病毒检测检出1例轮状病毒(A型)阳性,检出率为2.0%(1/50)。结论蝇类携带大量细菌,有些还携带肠道病毒,可导致疾病在国境口岸传播的风险,卫生检疫机构应加强对国境口岸蝇类检测,防止传染病扩散。
Objective To investigate the pathogens carried by flies at Tibetan ports, and to provide a technical support for the health quarantine and law enforcement at ports. Methods From May to October, 2017, pathogen detection was performed for flies collected at Lhasa Gonggar International Airport, Jilong port, and Yadong port. Bacterial detection was performed for fly samples using the microbiological isolation and pure culture technology, 16 S rRNA gene sequence identification, and physiological and biochemical identification techniques, and RT-PCR was used to detect the virus carried by flies. Results A total of 37 pathogenic bacteria(conditioned pathogens) were isolated from flies, including Enterococcus faecalis, Hafnia alvei, Moellerella wisconsensis, Escherichia coli, Proteus vulgaris, and Enterococcus durans.Virus detection found one case of rotavirus(group A), resulting in a detection rate of 2.0%(1/50). Conclusion Flies carry a large number of bacteria, and some of them may also carry enterovirus, which may lead to the risk of disease spread at frontier ports. Health quarantine departments should strengthen the monitoring and detection of flies at frontier ports to prevent the spread of infectious diseases.
引文
[1]高玉峰,程晓兰,孙时,等.辽宁口岸蝇类、蜚蠊携带致病菌的检测分析[J].中国国境卫生检疫杂志,2015,38 Suppl1:S22-25.DOI:10.16408/j.1004-9770.2015.S1.008.
[2]高思维,周天喜,高博,等.福州海港口岸蝇类携带病毒状况的监测分析[J].中国国境卫生检疫杂志,2011,34(1):31-34.DOI:10.16408/j.1004-9770.2011.01.014.
[3]黄建可,杨寿旺.中国主要蝇类携带致病菌情况与防制原则[J].口岸卫生控制,2009,14(2):52-55.DOI:10.3969/j.issn.1008-5777.2009.02.020.
[4]张洋洋,梁丽玲.医院获得性肠球菌属细菌感染的分布特征及耐药性分析[J].承德医学院学报,2018,35(2):101-104.DOI:10.15921/j.cnki.cyxb.2018.02.005.
[5]张欣,田素飞,陈佰义,等.某院感染科病房住院患者常见病原菌分布及耐药性分析[J].中国微生态学杂志,2018,30(2):168-171.DOI:10.13381/j.cnki.cjm.201802011.
[6]孙立新,丁永键,朱临,等.江苏口岸输入性蝇类携带细菌监测研究[J].中华卫生杀虫药械,2011,17(3):190-194.
[7]陈拓,曹晓梅,邓耀华,等.浦东机场口岸蝇类携带病原菌种类及16S r RNA鉴定[J].中国媒介生物学及控制杂志,2012,23(6):506-511.
[8]王卫平.儿科学[M].8版.北京:人民卫生出版社,2013:150-159.
