摘要
目的了解腹泻患者中副溶血性弧菌(Vibrio parahaemolyticus,VP)的检出情况,探讨流行优势型,分析副溶血性弧菌的毒力基因携带,从而了解疾病发生的诱因,为控制副溶血性弧菌引起的疾病提供依据。方法选择2017年5-10月分离培养腹泻患者标本中的病原菌;质谱仪鉴定出副溶血性弧菌菌株;利用脉冲场凝胶电泳(Pulsed Field Gel Electrophoresis,PFGE)进行分子分型;聚合酶链式反应(PCR)检测菌株毒力基因。结果 108份腹泻患者的粪便标本中培养分离副溶血性弧菌42株,检出率为38.89%(42/108)。PFGE分为12个型别,优势型为A1型,占59.52%(25/42)。tdh毒力基因阳性的副溶血性弧菌38株,阳性率为90.48%(38/42)。结论腹泻患者中副溶血性弧菌检出率较高,PFGE分型方法准确性高、特异性高、重复性好、结果易判读,致病性的菌株带型集中。患者中分离得到的副溶血性弧菌大多为带毒株,具有致病能力。
OBJECTIVE To understand the the detection of Vibrio parahaemolyticus in patients with diarrhea, detect predominant types in epidemics, analyze the carriage of toxic gene for learning the related factors of the infection and controling infections caused by V parahaemolyticus. METHODS V parahaemolyticus were isolated from patients with diarrhea from May 2017 to Oct. 2017 and identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS). Molecular characteristics were detected by pulsed field gel electrophoresis(PFGE). The toxic gene of V parahaemolyticus was detected using polymerase chain reaction(PCR). RESULTS 42 strains of V parahaemolyticus were isolated in the fecal specimens of 108 patients with diarrhea, and the detection rate was 38.89%(42/108). They were 12 types as found by PFGE methods, and the dominant type was A1, accounting for 59.52%(25/42). 38 strains of V parahaemolyticus carried tdh gene, and the positive rate was 90.48%(38/42). CONCLUSION The detection rate of V parahaemolyticus in patients with diarrhea is high. The PFGE classification method has high accuracy, specificity, good repeatability and easy interpretation. The pathogenic strains are concentrated in type. The Vibrio parahaemolyticus strains isolated from the patients are mostly virulent strains with pathogenic ability.
引文
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