摘要
Specimens of Clarias gariepinus were treated with lethal(70,75,80,85,90,and 95 mg/L) and sub-lethal concentrations(8,12 and 16 mg/L) of uranyl acetate,a low-radiotoxicity uranium salt. The LC 50 value was registered as 81.45 mg/L. The protein and glycogen concentrations in liver and muscles were decreased in the fish exposed to sub-lethal concentrations. The red blood cell(RBC) and white blood cell(WBC) counts,haemoglobin(Hb) concentration and haematocrit(Hct) values were decreased. Different blood indices like mean corpuscular volume(MCV),mean corpuscular haemoglobin(MCH) and mean corpuscular haemoglobin concentration(MCHC) were negatively affected. Level of plasma glucose was elevated whereas protein was decreased. The level of calcium concentration(Ca) was declined in the blood of exposed fish whereas magnesium(Mg) remains unchanged. The activity level of glutamic-oxaloacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) was elevated in exposed fish. These effects were more pronounced in the last period of exposure and in higher concentrations. Results of the present study indicate that uranyl acetate has adverse effects on C larias gariepinus and causes changes in the biochemical and hematological parameters of the fish.
Specimens of Clarias gariepinus were treated with lethal(70,75,80,85,90,and 95 mg/L) and sub-lethal concentrations(8,12 and 16 mg/L) of uranyl acetate,a low-radiotoxicity uranium salt. The LC 50 value was registered as 81.45 mg/L. The protein and glycogen concentrations in liver and muscles were decreased in the fish exposed to sub-lethal concentrations. The red blood cell(RBC) and white blood cell(WBC) counts,haemoglobin(Hb) concentration and haematocrit(Hct) values were decreased. Different blood indices like mean corpuscular volume(MCV),mean corpuscular haemoglobin(MCH) and mean corpuscular haemoglobin concentration(MCHC) were negatively affected. Level of plasma glucose was elevated whereas protein was decreased. The level of calcium concentration(Ca) was declined in the blood of exposed fish whereas magnesium(Mg) remains unchanged. The activity level of glutamic-oxaloacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) was elevated in exposed fish. These effects were more pronounced in the last period of exposure and in higher concentrations. Results of the present study indicate that uranyl acetate has adverse effects on C larias gariepinus and causes changes in the biochemical and hematological parameters of the fish.
引文
[CCME]Canadian Council of Ministers of the Environment.2011.Scientific Criteria Document for the Development of the Canadian Water Quality Guidelines for the Protection of Aquatic Life(Uranium).PN 1451,ISBN978-1-896997-97-1 PDF.Pp-xiv+106.
[FPTCDW]Federal-Provincial-Territorial Committee on Drinking Water.2001.Guidelines for Canadian Drinking Water Quality:Supporting Document for Uranium.Health Canada,Ottawa(ON).
[WWSA]World Wide Science Alliance.2011.Uranyl Acetate:Topics by World Wide Science.Org.http://worldwidescience.org/topicpages/u/uranyl+acetate.html.
Abou-Donia M B,Dechkovskaia A M,Goldstein L B et al.2002.Uranyl acetate-induced sensorimotor deficit and increased nitric oxide generation in the central nervous system in rats.Pharmacol.Biochem.Behav.,72(4):881-890.
Adeyemo O K.2007.Haematological profile of Clarias gariepinus(Burchell,1822)exposed to lead.T urk.J.Fish Aquat.Sci.,7(2):163-169.
Agrahari S,Pandey K C,Gopal K.2007.Biochemical alteration induced by monocrotophos in the blood plasma of fish,Channa punctatus(Bloch).Pest.Biochem.Physiol.,88(3):268-272.
Ahmad Z.2012.Toxicity bioassay and ef fects of sub-lethal exposure of malathion on biochemical composition and haematological parameters of C larias gariepinus.Afr.J.Biotech.,11(34):8 578-8 585.
Ahmad Z.2014.Biochemical and haematological changes induced by low-radiotoxicity uranium salt(Uranyl acetate)in H eteropneustes fossilis.J.Pure Appl.Micr.,8:593-603.
Al-Akel A S,Alkahem-Al-Balawi H F,Al-Misned F et al.2010.Eff ects of dietary copper exposure on accumulation,growth,and hematological parameters in C yprinus carpio.Toxicol.Environ.Chem.,92(10):1 865-1 878.
Al-Kahem H F.1995.Behavioural responses and changes in some haematological parameters of the cichlid fish,Oreochromis niloticus,exposed to trivalent chromium.J.King Abdul.Aziz Univ.Sci.,7(1):5-13.
Alkahemal-Balawi H F,Ahmad Z,Al-Akel A S et al.2011.Toxicity bioassay of lead acetate and ef fects of its sublethal exposure on growth,haematological parameters and reproduction in Clarias gariepinus.Afr.J.Biotech.,10(53):11 039-11 047.
Amer N,Alwachi S N.2012.Histological changes on the liver of the mothers treated with uranyl acetate in albino rats.Tikrit J.Pure Sci.,17(4):49-54.
Ashman P U,Seed J R.1973.Biochemical studies in the vole,Micritous montamus.I.The daily variation of hepatic-6-phosphatase and liver glycogen.C omp.Biochem.Physiol.,45:365-378.
Banaee M,Sureda A,MirvaghefiA R,Ahmadi K.2011.Ef fects of diazinon on biochemical parameters of blood in rainbow trout(Oncorhynchus mykiss).Pest.Biochem.Physiol.,99(1):1-6.
Barillet S,Adam C,Palluel O,Devaux A.2007.Bioaccumulation,oxidative stress,and neurotoxicity in Danio rerio exposed to diff erent isotopic compositions of uranium.E nviron.Toxicol.Chem.,26(3):497-505.
Black J A,Roberts R F,Johnson D M et al.1973.The significance of physicochemical variables in aquatic bioassays of heavy metals.In:Glass G E ed.Bioassay Techniques and Environmental Chemistry.Ann Arbor Science Publishers Inc.,Ann Arbor,MI.
Blaxhall P C,Daisley K W.1973.Routine haematological methods for use with fish blood.J.Fish Biol.,5(6):771-781.
Bradbury S P,Symonic D M,Coats J R,Atchison G J.1987.Toxicity of fenvalerate and its constituent isomers to the fathead minnow,P i m ephales prome la s,and bluegill,Lepomis macrochirus.Bull.Environ.Cont.Toxicol.,38(5):727-735.
Bywater J F,Banaczykowski R,Baily M.1991.Sensitivity to uranium of six species of tropical freshwater fishes and four species of cladocerans from northern Australia.Environ.Toxicol.Chem.,10(11):1 449-1 458.
Cambray R S,Bakins J D.1980.Studies of Environmental Radioactivity in Cumbria:Part I.Concentration of Plutonium and Caesium1 37 in Environmental Samples from West Cumbria and A Possible Maritime Ef fect.UKAtomic Energy Res.Establ.Harwell Rept.15p.
Cheng K L,Hogan A C,Parry D L et al.2010.Uranium toxicity and speciation during chronic exposure to the tropical freshwater fish,Mogurnda mogurnda.Chemosphere,79(5):547-554.
Cooley H M,Evans R E,Klaverkamp J F.2000.Toxicology of dietary uranium in lake whitefish(Coregonus clupeaformis).Aquat.Toxicol.,48(4):495-515.
D’Ilio S,Violante N,Senofonte O,Petrucci F.2007.Determination of depleted uranium in fish:validation of a confirmatory method by dynamic reaction cell inductively coupled plasma mass spectrometry(DRC-ICP-MS).A nal.Chem.Acta,597(2):195-202.
Daraie B,Pourahmad J,Hamidi-Pour N et al.2012.Uranyl acetate induces oxidative stress and mitochondrial membrane potential collapse in the human dermal fibroblast primary cells.I ran.J.Pharm.Res.,1 1(2):495-501.
Darolles C,Broggio D,Feugier A et al.2010.Dif ferent genotoxic profiles between depleted and enriched uranium.T oxicol.Lett.,192(3):337-348.
Domingo J L.2001.Reproductive and developmental toxicity of natural and depleted uranium:a review.R epr.Toxicol.,15(6):603-609.
Farmer G J,Ashfield D,Samont H S.1979.Eff ects of zinc on juvenile Atlantic salmon S almo Salar:acute toxicity,food intake,growth and bioaccumulation.E nviron.Pollut.,19(2):103-117.
Finney D J.1971.Probit Analysis.3r d edn.Cambridge Press,New York.
Gagnaire B,Bado-Nilles A,Sanchez W.2014.Depleted uranium disturbs immune parameters in zebra fish,Danio rerio:an ex vivo/in vivo experiment.A rch.Environ.Contam.Toxicol.,67(3):426-435.
Gilman A P,Villeneuve D C,Secours V E et al.1998.Uranyl nitrate:28 day and 91-day toxicity studies in the SpragueDawley rat.T oxicol.Sci.,41(1):117-128.
Goldman M,Yaari A,Doshnitzki Z et al.2006.Nephrotoxicity of uranyl acetate:ef fect on rat kidney brush border membrane vesicles.A rch.Toxicol.,80(7):387-393.
Goulet R R,Fortin C,Spry D J.2011.Uranium.In:Wood C M,Farrell A P,Brauner C J eds.Fish Physiology(Homeostasis and Toxicology of Non-Essential Metals).Elsevier Inc.31,p.391-428.
Guéguen Y,Souidi M,Baudelin C et al.2006.Short-term hepatic eff ects of depleted uranium on xenobiotic and bile acid metabolizing cytochrome P450 enzymes in the rat.Arch.Toxicol.,80(4):187-195.
Hamilton E I.1980.Concentration and distribution of Uranium in Mytilus edulis and associated materials.M ar.Ecol.Prog.Ser.,2:61-73.
Hamilton S J.1995.Hazard assessment of inorganics to three endangered fish in the Green River,Utah.E cotoxicol.Environ.Safety,30(2):134-142.
Hartsock W J,Cohen J D,Segal D J.2007.Uranyl acetate as a direct inhibitor of DNA-binding proteins.C hem.Res.Toxicol.,20(5):784-789.
Hinck J E,Linder G,Finger S et al.2010.Biological pathways of exposure and ecotoxicity values for uranium and associated radionuclides.I n:Alpine E A ed.Hydrological,Geological and Biological Site Characterization of Breccia Pipe Uranium Deposits in Northern Arizona.U.S.Geological Survey,Scientific Investigations Report2010-5025.354p.
Jaff er Ali H A,Rani V J.2009.Eff ect of phosalone on haematological indices in the tilapia,O reochromis mossambicus.Turk.J.Vet.Anim.Sci.,33(5):407-411.
Jeney G,Nemcsok J,Jeney Z S,Olah J.1991.Acute eff ect of sublethal ammonia concentrations on common carp(Cyprinus carpio L.).II.Eff ect of ammonia on blood plasma transaminases(GOT,GPT),G1DH enzyme activity,and ATP value.A quaculture,104(1-2):149-156.
Johnson C M,Toledo M C F.1993.Acute toxicity of endosulfan to the fish Hyphessobrycon bifasciatus and Brachydanio rerio.Archiv.Environ.Conta.Toxicol.,24(2):151-155.
Khangarot B S.1991.Toxicity of metals to a freshwater tubificid worm,Tubifex tubifex(Muller).B ull.Environ.Contam.Toxicol.,46(6):906-912.
Lowry O H,Rosebrough N J,Farr A L,Randall R J.1951.Protein measurement with the Folin phenol reagent.J.Biol.Chem.,1 9 3:265-275.
Mazeaud M M,Mazeaud F,Donaldson E M.1977.Primary and secondary eff ects of stress in fish:some new data with a general review.T rans.Am.Fish.Soc.,106(3):201-212.
Mc Kim J M,Christensen G M,Hunt E P.1970.Changes in the blood of brook Trout(Salvelinus fontinalis)after shortterm and long-term exposure to copper.C an.J.Fish.Res.Board,27(10):1 883-1 889.
Mc Kim J M.1985.Early life stage toxicity tests.I n:Rand GM,Petrocelli S R eds.Fundamentals of Aquatic Toxicology.Hemisphere Publishing,Washington DC.p.58-86.
Montgomery R.1957.Determination of glycogen.A rch.Biochem.Biophys.,67(2):378-386.
Mousa M M A,El-Ashram A M M,Hamed M.2008.Eff ects of Neem leaf extract on freshwater fishes and zooplankton community.I n:8 th International Symposium on Tilapia in Aquaculture.The Central Laboratory for Aquaculture Research,Cairo,Egypt.Oct.p.12-14.
Mulcahy M F.1975.Fish blood changes associated with disease:a hematological study of pike lymphoma and salmon ulcerative dermal necrosis.I n:Ribelin W E,Migaki Madison K eds.The Pathology of Fishes.University of Wisconsin Press,Madison.p.925-944.
Murty A S.1986.Toxicity of Pesticides to Fish.CRC Press Inc Boca Raton,FL.143p.
Nelson D L,Cox M M.2000.Lehninger Principles of Biochemistry.3r d edn.Worth Publishers,New York.
Nikolsky G V.1963.The Ecology of Fishes.Academic Press,London,New York.
Omoniyi I,Agbon A O,Sodunke S A.2002.Eff ect of lethal and sub-lethal concentrations of Tobacco(N icotiana tobaccum)leaf dust extract on weight and hematological changes in C larias gariepinus(Burchell).J.Appl.Sci.Environ.Manag.,6(2):37-42.
Ozmen M,Yurekli M.1998.Subacute toxicity of uranyl acetate in Swiss-Albino mice.E nviron.Toxicol.Pharmacol.,6(2):111-115.
Palanivelu P,Vijayavel K,Balasubramanian S E,Balasubramanian M P.2005.Influence of insecticidal derivative(cartap hydrochloride)from the marine polycheate on certain enzyme systems of the fresh water fish Oreochromis mossambicus.J.Environ.Biol.,26(2):191-195.
Parkhurst B R,Elder R G,Meyer J S et al.1984.An environmental hazard evaluation of uranium in a rocky mountain stream.E nviron.Toxicol.Chem.,3(1):113-124.
Periyakaruppan A,Kumar F,Sarkar S et al.2007.Uranium induces oxidative stress in lung epithelial cells.A rch.Toxicol.,81(6):389-395.
Priyamvada P,Khan S A,Khan M W et al.2010.Studies on the protective ef fect of dietary fish oil on uranyl-nitrateinduced nephrotoxicity and oxidative damage in rat kidney.Prostagl.Leukotr.Essent.Fatty Acids,82(1):35-44.
Rao D S.2010.Carbaryl Induced Changes in the Haematological,Serum Biochemical and Immunological Responses of Common Carp,Cyprinus carpio,(L.)with Special Emphasis on Herbal Extracts as Immunomodulators.Ph.D.Thesis,Andhra University,India.235p.
Rice T R,Baptist J P,Price T J.1965.Accumulation of mixed fission products by marine organisms.In:Pearson E A ed.Advances in Water Pollution Research.Pergamon Press,New York,Frankfurt-am-Main.
Roche H,BogéG.1996.Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication.M ar.Environ.Res.,41(1):27-43.
Salah El-Deen M A,Sharada H I,Abu-El-Ella S M.1996.Some metabolic alteration in grass carp(Ctenopharyngodon idella)induced by exposure to cadmium.J.Egypt.Ger.Soc.Zool.,21:441-457.
Schaedler C B.1981.Stress and compensation in teleostean fishes:response to social and physical factors.I n:Pickering A D ed.Stress and Fish.Academic Press,New York,USA.p.295-322.
Shekhanova I A.1980.Radiological aspects of surface water protection under condition of peaceful uses of nuclear energy.I n:Enko A I ed.Problems of Animal Ecology.Nauka Publication,USSR.
Simon O,Floc’h E,Geff roy B,Frelon S.2014.Exploring ecotoxicological fish bioassay for the evaluation of uranium reprotoxicity.E nviron.Toxicol.Chem.,33(8):1 817-1 824.
Skidmore J F.1965.Resistance to zinc sulphate of the zebra fish(Brachydani o rerio,Hamilton-Buchanan)at dif ferent phases of its life history.A nn.Appl.Biol.,56(1):47-53.
Stearns D M,Yazzie M,Bradley A S et al.2005.Uranyl acetate induces hprt mutations and uranium-DNA adducts in Chinese hamster ovary EM9 cells.M utag enesis,20(6):417-423.
Strange R J,Schreck C B,Golden J T.1977.Corticoid stress responses to handling and temperature in salmonids.Trans.Am.Fish.Soc.,106(3):213-218.
Van Vuren J H J.1986.The eff ects of toxicants on the haematology of L abeo umbratus(Teleostei:Cyprinidae).Comp.Biochem.Physiol.C:Comp.Pharm.,8 3(1):155-159.
Vicente-Vicente L,Quiros Y,Pérez-Barriocanal F et al.2010.Nephrotoxicity of uranium:pathophysiological,diagnostic and therapeutic perspectives.T oxicol.Sci.,118(2):324-347.
Webb J N,Levy H B.1955.A sensitive method for the determination of deoxyribonucleic acid in tissues and microorganisms.J.Biol.Chem.,213(1):107-117.
Winkaler E U,Santosh T R M,Machdo-Neto J G et al.2007.Acute lethal and sub-lethal ef fects of neem leaf extract on the neotropical freshwater fish Prochilodus lineatus.Comp.Biochem.Physiol.C:Toxicol.P harm.,145(2):236-244.
Yazzie M,Gamble S L,Civitello E R,Stearn D M.2003.Uranyl acetate causes DNA single strand breaks in vitro in the presence of ascorbate(vitamin C).C hem.Res.Toxicol.,16(4):524-530.
Zaki M S,Mostafa S O,Nasr S et al.2009.Biochemical,clinicophathlogical and microbial changes in C larias gariepinus exposed to pesticide malathion and climate changes.Rep.Opn.,6-11.
Zymmerman K L,Barber D S,Ehrich M F et al.2007.Temporal clinical chemistry and microscopic renal ef fects following acute uranyl acetate exposure.T oxicol.Path.,35(7):1 000-1 009.
