2013—2018年中国H7N9流感病毒HA与NA进化研究

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英文篇名：Evolution of HA and NA of H7N9 influenza virus in China( 2013-2018) 作者：安淑一 ; 孙英伟 ; 姚文清 英文作者：AN Shuyi;SUN Yingwei;YAO Wenqing;Liaoning Provincial Center for Disease Control and Prevention; 关键词：H7N9 ; 血凝素(HA) ; 神经氨酸酶(NA) ; 分子进化 ; 突变 英文关键词：H7N9;;Hemagglutinin(HA);;Neuraminidase(NA);;Molecular evolution;;Mutation 中文刊名：FBYF 英文刊名：Journal of Public Health and Preventive Medicine 机构：辽宁省疾病预防控制中心; 出版日期：2019-06-30 出版单位：公共卫生与预防医学 年：2019 期：v.30;No.169 基金：国家重大传染病防治专项项目(2017ZX10103007001);; 辽宁省自然科学基金计划重点项目(20170540488) 语种：中文; 页：FBYF201903003 页数：6 CN：03 ISSN：42-1734/R 分类号：16-21

摘要

目的研究2013—2018年中国H7N9流感病毒HA与NA进化研究。方法通过收集NCBI网站HA与NA序列数据,对各年份筛选出序列进行进化分析、关键位点分析,糖基化位点分析。结果 H7N9流感病毒HA与NA序列中韩与美国形成两大分支。韩国与中国形成并列分支。2013—2018年间遗传距离增加,同源性递减。所有中国株缺失69～73位缺失"QISNT"。NA糖基化位点42～45位,关键位点193有突变。HA抗原决定簇148位点,182和183位点有变异,关键位点186和134有突变。结论中国H7N9流感病毒HA与NA序列正在变迁,部分关键位点已经发生突变,可能会改变病毒的致病特征。
        Objective To study the molecular epidemiology of HA and NA proteins of H7N9 influenza virus in China from 2013 to2018,and to analyze the evolution characteristics of H7N9 influenza virus. Methods The NC and NA sequence data were collected from NCBI website The selected sequences in each year were analyzed by evolutionary analysis,key site analysis and glycosylation site analysis. Results The HA and NA sequences of H7N9 influenza virus formed two branches in China,Korea and the United States.South Korea and China formed parallel branches. The genetic distance increased,and the homology decreased between 2013 and 2018.All Chinese strains had a deletion of sequence 69-73 " QISNT". NA glycosylation sites 42-45 and key site 193 had mutations. In addition,there were mutations at site 148 of HA epitope,sites 182 and 183,and key sites 134 and 186. Conclusion The HA and NA sequences of H7N9 influenza virus in China were changing,and some key sites have mutated,which may change the pathogenic characteristics of the virus.

引文

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