摘要
目的探讨果糖-1,6-二磷酸酶1(fructose-1,6-bisphosphatase 1, FBP1)在结肠癌及其癌旁组织中的表达,以及在结肠癌侵袭及有氧糖酵解中的作用和机制。方法应用组织芯片技术结合免疫组织化学染色方法检测FBP1表达,对结果及临床病理学参数进行统计学分析;干扰结肠癌SW480细胞FBP1表达,CCK-8检测其细胞增殖,Transwell实验检测细胞侵袭能力,细胞能量代谢实验检测细胞糖酵解能力。结果 FBP1在80例结肠癌组织中高表达率显著低于80例癌旁肠黏膜组织(21.25%vs 86.25%,P<0.01);CCK-8检测结果显示FBP1可以抑制结肠癌SW480细胞的增殖能力(P<0.01); Transwell实验结果显示FBP1可以抑制SW480细胞的侵袭能力(P<0.01);细胞能量代谢实验结果显示FBP1可以抑制SW480的糖酵解水平。结论 FBP1蛋白在结肠癌组织中表达低于癌旁肠黏膜组织,其能抑制结肠癌SW480细胞的侵袭以及有氧糖酵解。
Objective To determine the expression of fructose-1,6-bisphosphatase 1(FBP1) in colon cancer tissues, and investigate its roles and underlying mechanisms in the invasion and aerobic glycolysis of colon cancer cells. Methods The expression of FBP1 was detected by tissue microarray combined with immunohistochemical staining, and then the results were statistically analyzed with clinical pathological parameters. In the colon cancer SW480 cells with vector control, FBP1 overexpression or knock-down, CCK-8 assay was used to evaluate cell proliferation, Transwell chamber test was employed to detect their cell invasion ability, and cell energy metabolism test was applied to measure cell glycolysis. Results The higher expression of FBP1 in 80 cases of colon cancer tissues was significantly lower than that in 80 para-cancerous tissues(21.25% vs 86.25%, P<0.01). The results of CCK-8 assay showed that FBP1 inhibited the proliferation of SW480 cells(P<0.01). Transwell assay indicated that FBP1 inhibited the invasion of SW480 cells(P<0.01). Cell energy metabolism test showed that FBP1 suppressed the level of glycolysis in SW480 cells. Conclusion FBP1 is lowly expressed in colon cancer tissues compared to para-cancerous tissues. FBP1 inhibits migration, invasion and aerobic glycolysis of colon cancer SW480 cells.
引文
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