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清肺消炎丸对不可分型流感嗜血杆菌诱导肺部炎症小鼠的影响
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- 英文篇名:Effects of Qingfei Xiaoyan Pills on lung inflammation mice induced by non-typeable Haemophilus influenzae
- 作者:黄家望 ; 陈平安 ; 廖灿 ; 袁娉 ; 谢希 ; 孟亚飞 ; 付涵煦 ; 曲磊 ; 刘淑
- 英文作者:HUANG Jia-wang;CHEN Ping-an;LIAO Can;YUAN Ping;XIE Xi;MENG Ya-fei;FU Han-xu;QU Lei;LIU Shu;College of Integration of Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine;College of Medicine, Hunan University of Chinese Medicine;College of Traditional Chinese Medicine, Hunan University of Chinese Medi-cine;Darentang Pharmaceutical Factory, Tianjin Zhongxin Pharmaceutical Group Co., Ltd.;
- 关键词:清肺消炎丸 ; 不可分型嗜血流血杆菌 ; 肺部炎症
- 英文关键词:Qingfei Xiaoyan Pills;;non-typeable Haemophilus influenzae;;lung inflammation
- 中文刊名:ZCYA
- 英文刊名:Chinese Traditional Patent Medicine
- 机构:湖南中医药大学中西医结合学院;湖南中医药大学医学院;湖南中医药大学中医学院;天津中新药业集团股份有限公司达仁堂制药厂;
- 出版日期:2019-05-20
- 出版单位:中成药
- 年:2019
- 期:v.41
- 基金:湖南省大学生研究性学习与创新性实验项目(2017-292,2018-412);;
湖南中医药大学大学生研究性学习与创新性实验项目(2017-06,2018-01,2018-31,2018-47)
- 语种:中文;
- 页:ZCYA201905010
- 页数:7
- CN:05
- ISSN:31-1368/R
- 分类号:48-54
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摘要
目的探讨清肺消炎丸对不可分型流感嗜血杆菌诱导肺部炎症小鼠的影响。方法 50只小鼠随机分为空白组、模型组、氨苄西林组(0.288 mg/kg)、麻杏石甘汤组(6.05 g/kg)、清肺消炎丸组(2.16 g/kg),每组10只。给药7 d后,测定一般情况(体质量及肺、脾、胸腺指数),HE染色观察肺组织病理变化,ELISA法检测血清炎性细胞因子(IL-1β、IL-6、GCSF、TNF-α)水平,Western blot及RT-PCR法分别检测肺组织TLR4、MyD88、TRAF6蛋白及mRNA表达。结果与模型组比较,清肺消炎丸组一般情况显著改善(P<0.01),炎性细胞因子水平,TLR4、MyD88、TRAF6蛋白及mRNA表达显著降低(P<0.01)。结论清肺消炎丸可缓解不可分型流感嗜血杆菌诱导肺部炎症小鼠肺损伤和炎症,其机制可能与调控被激活的TLR4-MYD88-TRAF6信号通路、抑制炎性细胞因子分泌有关。 AIM To explore the effects of Qingfei Xiaoyan Pills on lung inflammation mice induced by non-typeable Haemophilus influenzae.METHODS Fifty mice were randomly divided into blank group, model group, ampicillin group(0.288 mg/kg), Maxing Shigan Decoction group(6.05 g/kg) and Qingfei Xiaoyan Pills group(2.16 g/kg), 10 mice in each group. After 7-day administration, general conditions(body weight and lung, spleen, thymus indices) were determined, pathological changes in lung tissue were detected by HE staining, the levels of serum inflammatory cytokines(IL-1β, IL-6, GCSF, TNF-α) were detected by ELISA, and TLR4, MyD88, TRAF6 protein and mRNA expressions in lung tissue were detected by Western blot and RT-PCR, respectively.RESULTS Compared with the model group, the Qingfei Xiaoyan Pills group demonstrated significant improvement in general conditions(P<0.01), and marked decrease in inflammatory cytokine levels, TLR4, MyD88, TRAF6 protein and mRNA expressions(P<0.01).CONCLUSION Qingfei Xiaoyan Pills can alleviate lung injury and inflammation in lung inflammation mice induced by non-typeable Haemophilus influenzae, whose mechanisms may contribute to the regulation of TLR4-MYD88-TRAF6 signaling pathway and inhibition of inflammatory cytokine secretion.
引文
[1] Sethi S.Infectious etiology of acute exacerbations of chronic bronchitis[J].Chest,2000,117(5 Suppl 2):380S-385S. [2] St Geme J W.Molecular and cellular determinants of non-typeable Haemophilus influenzae adherence and invasion[J].Cell Microbiol,2002,4(4):191-200. [3] Leroux-Roels G,Van Damme P,Haazen W,et al.PhaseⅠ,randomized,observer-blind,placebo-controlled studies to evaluate the safety,reactogenicity and immunogenicity of an investigational non-typeable Haemophilus influenzae (NTHi) protein vaccine in adults[J].Vaccine,2016,34(27):3156-3163. [4] Khan M N,Ren D,Kaur R,et al.Developing a vaccine to prevent otitis media caused by nontypeable Haemophilus influenzae[J].Expert Rev Vaccines,2016,15(7):863-878. [5] Clancy R L,Dunkley M L,Sockler J,et al.Multi-site placebo-controlled randomised clinical trial to assess protection following oral immunisation with inactivated non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease[J].Intern Med J,2016,46(6):684-693. [6] 何金鑫,黄丽华.LPS-TLR4/NF-κB信号通路与抗炎免疫研究进展[J].中外健康文摘,2013,6(37):27-29. [7] 张玲玲,史欣德.小柴胡汤治疗发热的调查分析[J].辽宁中医学院学报,2003,5(4):311-313. [8] Bakaletz L O,Leake E R,Billy J M,et al.Relative immunogenicity and efficacy of two synthetic chimeric peptides of fimbrin as vaccinogens against nasopharyngeal colonization by nontypeable Haemophilus influenzae in the chinchilla[J].Vaccine,1997,15(9):955-961. [9] 李玲,卢芳国,熊兴耀,等.麻杏石甘汤对A型流感病毒感染小鼠的免疫保护作用[J].中医药学报,2010,38(2):25-28. [10] Sethi S.Bacterial infection and the pathogenesis of COPD[J].Chest,2000,117(5 Suppl 1):286S-291S. [11] Medzhitov R,Preston-Hurlburt P,Janeway C A.A human homologue of the Drosophila Toll protein signals activation of adaptive immunity[J].Nature,1997,388(6640):394-397. [12] Alexopoulou L,Holt A C,Medzhitov R,et al.Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3[J].Nature,2001,413(6857):732-738. [13] Di Domizio J,Blum A,Gallagher-Gambarelli M,et al.TLR7 stimulation in human plasmacytoid dendritic cells leads to the induction of early IFN-inducible genes in the absence of typeⅠIFN[J].Blood,2009,114(9):1794-1802. [14] Akira S,Hemmi H.Recognition of pathogen-associated molecular patterns by TLR family[J].Immunol Lett,2003,85(2):85-95. [15] 李玲,魏科,卢芳国,等.基于TLR4-MyD88-TRAF6信号通路的麻杏石甘汤抗A型流感病毒感染小鼠所致的病毒性肺损伤研究[J].中草药,2017,48(8):1591-1596. [16] Wang J Q,Jeelall Y S,Ferguson L L,et al.Toll-like receptors and cancer:MYD88 mutation and inflammation[J].Front Immunol,2014,5:367. [17] Xu C Q,Chen G,Yang W W,et al.Hyaluronan ameliorates LPS-induced acute lung injury in mice via Toll-like receptor (TLR)4-dependent signaling pathways[J].Int Immunopharmacol,2015,28(2):1050-1058. [18] 张弦,庞浩龙,于旭红.清肺消炎丸的临床应用评价[J].中国医院用药评价与分析,2013,13(9):780-781. [19] 程彬峰.清肺消炎丸抗炎、平喘活性物质筛选及其作用机制研究[D].天津:南开大学,2013.
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