摘要
目的用HPLC-ELSD法测定磷脂酰丝氨酸和磷脂酰胆碱的含量。方法色谱柱为LiChrospher 100Diol(100mm×4.6mm,5μm);流动相A正己烷-异丙醇-冰醋酸-三乙胺(825∶170∶5∶0.8)以及流动相B异丙醇-水-冰醋酸-三乙胺(845∶150∶5∶0.8),A、B两相梯度洗脱;流速:1.0mL·min~(-1);检测器漂移管温度:85℃;气体流速:2.5L·min~(-1);柱温:35℃。结果磷酯酰丝氨酸与磷脂酰胆碱的线性范围为0.1~0.8mg·mL~(-1);相关系数分别为0.999 8和0.999 6。磷脂酰丝氨酸的加样平均回收率为96.40%(RSD=2.31%),磷脂酰胆碱的加样平均回收率为96.58%(RSD=1.55%)。结论该方法专属性高,定量准确,可作为控制磷脂酰丝氨酸和磷脂酰胆碱含量的方法。
Objective To establish a method for determining the content of phosphatidylserine and phosphatidyl cholines by HPLCELSD.Methods Column:LiChrospher 100 Diol(100 mm×4.6 mm,5μm);mobile phase:N-hexane-isopropanol-glacial acetic acid-triethylamine(825∶170∶5∶0.8,A phase)and isopropanol-water-acetic acid-triethylamine(845∶150∶5∶0.8,B phase)using gradient elution.The flow rate was 1.0 mL·min~(-1) and detected by ELSD.Results The linear range of phosphatidylserine was0.1-0.8 mg·mL~(-1) with correlation coefficient 0.999 8.The linear range of phosphatidyl cholines was 0.1-0.8 mg·mL~(-1) with correlation coefficient 0.999 6.The average recovery of phosphatidylserine was 96.40%(RSD=2.31%),and the average recovery of phosphatidyl cholines was 96.58%(RSD=1.55%).Conclusion The method has high specificity and accuracy,and can be used as a method for controlling the content of phosphatidylserine and phosphatidyl cholines.
引文
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