摘要
目的凶险性前置胎盘(PPP)合并胎盘植入严重威胁孕产妇的生命安全。文中探讨锰超氧化物歧化酶(MnSOD)、去乙酰化酶3(SIRT3)的改变与SIRT3在PPP伴胎盘植入孕产妇胎盘中的表达,及其在滋养细胞侵袭与胎盘植入的联系。方法收集2014年1月至2018年6月安徽省妇幼保健院90例PPP合并胎盘植入孕产妇胎盘组织,按照胎盘绒毛侵入子宫肌层深度分为粘连组(30例)、植入组(30例)及穿透组(30例);对照组纳入正常胎盘组织30例。采用免疫组织化学SP法和Western blot法检测研究组和对照组胎盘组织中MnSOD与SIRT3的表达,并进行比较分析。结果免疫组化和Western blot结果表明,与对照组相比较,胎盘植入组中MnSOD与SIRT3表达明显,且随着胎盘植入程度的增加而降低;Western blot结果表明,对照组MnSOD/β-actin与SIRT3/β-actin相对蛋白表达分别为(0.39±0.05)、(0.41±0.04),均分别高于粘连组[(0.35±0.04)、(0.32±0.02)]、植入组[(0.28±0.02)、(0.20±0.03)]和穿透组[(0.23±0.01)、(0.17±0.02)],差异均有统计学意义(P<0.05)。结论PPP合并胎盘植入胎盘组织及侵袭的滋养细胞中MnSOD与SIRT3表达明显降低,两者均参与了胎盘植入的发生发展过程。
Objective Dangerous placenta previa(PPP)combined with placenta implantation seriously threatens maternal life safety. This paper aim to explore the changes of MnSOD and SIRT3,the expression of SIRT3 in maternal placenta PPP combined with placenta implantation,and the relationship between trophoblast invasion and placental implantation.Methods 90 cases with placenta implantation of pernicious placenta previa were collected from January 2014 to June 2018 in Anhui Maternal and Child Health Hospital. According to the depth of placental villus invading uterine myometrium,30 cases of placenta adhesion,30 cases of placental implantation,and 30 cases of placenta penetration,30 cases of normal control group.Immunohistochemical SP and Western blot were used to detect the expression of MnSOD and SIRT3 in placental tissues of the study group and the control group,then compared and analyzed.Results Compared with the control group,the expression of MnSOD and SIRT3 in the placental implantation group were increased. With the increasing of placental implantation degree,the level of MnSOD and SIRT3 decreased significantly(P<0.05). Western blot showed that,the relative protein expressions of MnSOD/β-actin and SIRT3/β-actin in the control group were(0.39±0.05)and(0.41±0.08),which were higher than those in the adhesion group[(0.35±0.04),(0.32±0.02)],the implantion group[(0.28±0.02),(0.20±0.03)],and the penetration group[(0.23±0.01),(0.17±0.02)]. The difference was statistically significant(P<0.05).Conclusion The expressions of MnSOD and SIRT3 incytoplasm or nucleus of invasive trophoblasts and placental tissues of pregnant women with placental implantation is significantly decreased,both of which are involved in the occurrence and development of placental implantation,but the specific pathogenesis still needs to be further explored.
引文
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