摘要
中医学认为肾虚是骨质疏松症的主要病因,中医药在治疗该病中占有重要地位。近年来,运用骨质疏松症易感基因的调控机理来探讨中医药防治该病的机制是当前的研究热点之一。笔者主要从骨质疏松易感基因方面对中医药防治骨质疏松的机制做一综述。
In the theory of traditional Chinese medicine( TCM),kidney deficiency is the main cause of osteoporosis. TCM plays an important role in the treatment of this disease. In recent years,using the regulation mechanism of osteoporosis-related genes in exploring the mechanism of TCM in preventing and treating this disease is one of the hot research topics. This paper review s the mechanism of TCM in preventing and treating osteoporosis mainly from the aspect of osteoporosis-related genes.
引文
[1]LIU Zhonghou.The pathogenesis of Osteoporosis.Beijing:Science Press,1998:142.
[2]ZHENG Hongxin,YAN Yan,WANG Sicheng,et al.Regulation of Expressions of TGF-β1,TIEG1 mRNA and Protein by KidneyTonifying Chinese Herbs in Osteoporosis Rats Induced by Ovarian Removal.Modernization of Traditional Chinese Medicine and Materia Medica—WORLD SCIENCE AND TECHNOLOGY,2010(1):57-64.
[3]LAI Manxiang.Exploration of the mechanism of traditional Chinese medicine on osteoporosis.Chinese Journal of Modern Drug Application,2010(3):235-236.
[4]TIAN Jian,KANG Xuepan.Research status and Prospect of treatment of primary osteoporosis with combined traditional Chinese and Western Medicine.Journal of Shanxi College of Traditional Chinese Medicine,2011,12(4):76-78.
[5]BAI Xue,WANG Yi,YU Shunlu.Progress of the study on the genes related to osteoporosi.China Journal of Orthopaedics and Traumatology,2006,19(9):573-576.
[6]Buit roga C,V azquez G,De Bo land AR,et al.The Vitam in D recep2 to rmediates,Rapid changes in musclep rotein lyro sine phospho ry2 lat ion induced by 1,25(OH)2D3.Biophys Res Conurron,2001,289(5):1150-1156.
[7]Morrison NA,Qi JC,Tokita A,et al.Prediction of bone density from vitamin D receptor alleles.Nature,1994,367(6460):284-287.
[8]Ralston SH,Uitterlinden AG,Brandi ML,et al.Large-scale evidence for the effect of the COLIA1 Sp1 polymorphism on osteoporosis outcomes:the GENOMOS study.PLoS Med,2006,3(4):90.
[9]LUAN Junwei,CHEN Xiaoliang,YUAN Baisheng,et al.Association between calcitonin receptor gene polymorphism and primary osteoporosis.Journal of Clinical Rehabilitative Tissue Engineering Research,2008,12(7):1243-1246.
[10]Funakoshi Y,Omori H,Yada H,et al.Relationship between changes of BMD over seven years and A1330V polymorphism of the low-density LPR5 gene in Japanese female workers.Asia Pac J Clin Nutr,2010,19(4):534-539.
[11]Karsak M,Malkin I,Toliat MR,et al.The cannabinoid receptor type 2(CNR2)gene is associated with hand bone strengthphenotypes in an ethnically homogeneous family sample.Hum Genet,2009,126(5):629-636.
[12]GE Jirong,LI Shengqiang,ZHU Xiaoxiang,et al.BsmⅠpolymorphism of vitamin D receptor gene and traditional Chinese medicine differentiation type in relation to bone mineral density in female patients with postmenopausal osteoporosis.Chinese Journal of Clinical Rehabilitation,2006,10(15):42-44.
[13]WANG Litong,ZHAO Yongfang,WANG Xiang,et al.Effect of Kidney-Nourishing and Spleen-Invigorating Formula on VDR mRNA expression in Kidney and Femur of Ovariectomized Rats.Chinese Journal of Traditional Medical Traumatology&Orthopedics,2009(4):4-7.
[14]REN Lu,ZHENG Hongxin,DU Song,et al.Research on the expression of the 1,25(OH)_2D_3 receptor mRNA and protein in the intestinal mucosa of the osteoporosis rats due to kidney deficiency affected by nanometer calcium combined with tonifying kidney herbs.Chinese Journal of Osteoporosis,2007(9):663-666,654.
[15]WANG Xiang,ZHAO Yongfang,SHI Yinyu,et al.The Effects of JianPi prescription on Vitamin D_3 metabolism in ovariectomized rats.Chinese Journal of Osteoporosis,2007(6):429-432,413.
[16]GE Jirong,WANG Heming,ZHU Xiaoxiang,et al.Effect of PvuⅡpolymorphisms of estrogen receptor gene on filtering risk factors in postmenopausal osteoporosis.Chinese Journal of Osteoporosis,2006,12(1):38-40.
[17]LI Shengqiang,GE Jirong,XIE Bingying,et al.Relationship between polymorphism of estrogen receptor alpha gene and bone mineral density in Fuzhou elderly male:Analysis of 150 cases.Journal of Clinical Rehabilitative Tissue Engineering Research,2009,13(37):7326-7329.
[18]Qu Q,Perala HM,Kapanen A,et al.Est rogen enhances diferent i2 at ion of o steoblast in mouse bone marrow culture.Bone,1998,22(3):201.
[19]Lim SK,Won YJ,Lee HC,et al.A PCR analysis of ER alpha and ER beta mRNA abundence on rats and the efect of ovariectomy.J BoneM iner Res,1999,14(7):1189.
[20]GE Quansheng,ZHU Yinghui,WANG Shouyu,et al.Influence of Chinese medicine of Jiangzhi Zhuanggu on bone morphogenetic protein and estrogen receptor expression in bone tissue of hyperlipidemia-induced osteoporosis rats.Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(7):1267-1271.
[21]YANG Lijuan,HUANG Junmei,WANG Fei.Evaluation of the phytoestrogenic activity of Polygoni Multiflori Radix,Drynariae Rhizoma and Epimedii Folium.Pharmacy and Clinics of Chinese Materia Medica,2012(3):37-40.
[22]ZHAI Yuankun,PAN Yalei,NIU Yinbo,et al.Comparative studies on the differentiation and maturation of rat calvarial osteoblasts by psoralen and isopsoralen in vitro.Chinese Pharmacological Bulletin,2012,28(3):355-361.
[23]BIAN Qin,LIU Shufen,HUANG Jianhua,et al.Regulation and control of three active components of TCM effective in reinforcing kidney on ovariotomy-induced osteoporosis rat s bone marrow stem cells revealed by gene microarray.China Journal of Traditional Chinese Medicine and Pharmacy,2011(5):889-893.
[24]LIU Lipeng,WU Jie.Advances in research on the relationship between osteoporosis growth factor-βand postmenopausal transformation.Chinese Journal of Practical Gynecology and Obstetrics,2011,27(5):386-388.
[25]PAN Guichao,WANG Huiqi,ZHANG Zhiguo.The effect of Bushen Jianpi Huoxue Recipe on osteoporotic fracture healing in osteoporosis rats and exploration of mechanisms.Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2011(2):156-157.
[26]WU Mishan,LI En,ZHAO Suzhi,et al.Meridian tropism of prescription for tonifying kidney and the expression of signal transduction protein Smad 4 in target organs following experimental osteoporosis.Journal of Clinical Rehabilitative Tissue Engineering Research,2007(45):9095-9099.
[27]WU Mishan,LI En,ZHAO Suzhi,et al.Meridian tropism of prescription for tonifying kidney and the expression of signal transduction protein in target organs following experimental osteoporosis.Journal of Clinical Rehabilitative Tissue Engineering Research,2007(14):2691-1694,2701.
[28]HU Jing,ZHENG Hongxin.Regulating Effect of Serum Containing Oyster Calcium and Kidney-reinforcing Chinese Herbs on Signal Transduction of Bone Formation Induced by BMP-4.Chinese Journal of Traditional Medical Traumatology&Orthopedics,2009(7):1-4.
[29]SHANG Deyang,ZHENG Hongxin,ZONG Zhihong,et al.Experimental research on expression of smurf1 mRNA and protein in kidney of the osteoporosis rats using ovariectomy with kidneydeficiency.Chinese Journal of Osteoporosis,2008(10):704-707,699.
[30]SHANG Deyang,ZHENG Hongxin,ZONG Zhihong,et al.Experimental Research on Expression of Smurf2 mRNA and Protein in Kidney of the Osteoporosis Rats Using Ovariectomy with Kidney-deficiency.Chinese Archives of Traditional Chinese Medicine,2008(8):1684-1687.
[31]GUO Bangfu,LUO Jiang,SHAO Min,et al.Effect of Serum Containing Chinese Medicine Gukang on OPG and RANKL mRNA Expression in In-vitro Cultured Human Osteoblasts.Journal of Guangzhou University of Traditional Chinese Medicine,2012(4):392-396,487.
[32]NIU Yu,ZHENG Hongxin.The effect of traditional Chinese medicine on expression of mRNA and protein of D9K of renal tissue in ovariectomized rats.Jilin Journal of Traditional Chinese Medicine,2012(1):68-69.
[33]LIN Yifeng,LIU Qingsi.Effect of Gukang on the expression of PDGF-AA in osteoblasts of newborn rats on experimental research.Chinese Journal of Osteoporosis,2006(5):459-461.
[34]LIN Yifeng,LIANG Zujian,HE Mingtao.Effect of Bushen Tongluo Prescription on Osteoclast Cathepsin K in Ovariectomized Rats.Journal of Guangzhou University of Traditional ChineseMedicine,2010(4):371-374,383,439.
[35]Liu JM,Zhang MJ,Zhao L,et al.Analysis of recently identified osteo-porosis susecptibility genes in Han Chinese women.J Clin Eno-docrinol Metab,2010,95(9):112-120.
[36]Mencej-Bedrac S,Prezelj J,Kocjan T,et al.Analysis of association of LRP5,LRP6,SOST,DKK1,and CTNNB1 genes with bone min-eral density in a Slovenian population.Calcif Tissue Int,2009,85(6):501-506.
[37]GE Jirong,XIE Lihua,CHEN Ke,et al.Analysis of differential expression genes in postmenopausal osteoporosis patients with Kidney Yang deficiency.Chinese Journal of Osteoporosis,2012(2):134-138.
[38]XIE Lihua,ZHAO Yipeng,CHEN Ke,et al.Bioinformaticsanalysis of genes expression profiles of postmenopausal osteoporosis with Kidney Yin deficiency.Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(15):2833-2837.
[39]CHEN Xi,LIANG Zujian,SHAO Min,et al.Regulatory effect of Bushen Jianpi Huoxue Recipe on signal transduction gene expression of osteoporosis.Journal of New Chinese Medicine,2008,40(3):60-62.
[40]LIANG Zujian,HAN Qingmin,ZHUANG Hong.Preliminary analysis of expression profiles of signal transduction related gene in osteoporosis by cDNA microarry.Chinese Journal of Osteoporosis,2007,13(9):631-634,617.