摘要
通过透明圈比较法从土壤中筛选出产木聚糖酶的菌株36株,以玉米芯为唯一碳源进行液体摇瓶发酵,对其中一株产酶水平较高的霉菌FH2213进行发酵条件的优化并对粗酶液的性质进行初步研究。采用Setp-by-step单因素试验法,获得FH2213产木聚糖酶的最佳发酵培养条件:碳源为玉米芯(15 g/L),氮源为酵母膏(15 g/L),初始pH为3.0,培养温度为30℃。在最适培养条件下发酵5 d木聚糖酶活达200 U/mL。FH2213产木聚糖酶的最适反应温度为65℃,最适反应pH为5.4。另外,SDS-PAGE和酶谱分析结果表明该菌产生3种木聚糖酶,分子量约为20.4,21.7以及34.3 kDa。
Thirty-six xylanase-producing strains were isolated from soils with the single carbon source corncobs, and strain FH2213 showed the highest yield on xylanase production. Its fermentation conditions were optimized by single factor experiment method step-by-step and the characterization of xylanase had been studied preliminarily. At Optimal fermentation condition for 5 d with initial pH 3.0 and temperature 30 ℃, 1.5% corncob, 1.5% yeast extract, the xylanase activity was up to 200 U/mL. The optimal reaction temperature and pH were 65 ℃ and 5.4, respectively. In addition, SDS-PAGE and zymography analysis revealed that FH2213 produced 3 kinds of xylanases with molecular mass about 20.4, 21.7 and 34.3 kDa, respectively.
引文
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