摘要
目的讨论肝癌组织中希佩尔林道(von Hippel-Lindau,VHL)的表达及其临床意义。方法采用免疫组化两步法检测VHL蛋白在40例肝癌患者癌组织和癌旁组织及10例正常肝组织中的表达,并分析VHL蛋白的表达与临床指标的关系。结果肝癌组织中VHL表达阳性率(45%)显著低于对应癌旁组织(82.5%)(χ~2=12.17,P<0.0001)。正常肝组织中VHL表达阳性率90%。VHL蛋白的表达与血管侵犯显著相关。肝癌组织中VHL表达阳性和阴性1年、3年、5年的总体生存率分别为83.3%、61.1%、55.5%和59.1%、50%、40.9%,差异无统计学意义(P=0.228)。结论 VHL蛋白在肝癌中的表达与肿瘤的生物学行为及预后密切相关。
Objective To investigate the expression and clinical significance of von Hippel-Lindau protein in hepatocellular carcinoma. Methods Immunohistochemistry was used for detecting the expression of VHL in HCC tissues(n = 40) and normal hepatic tissues(n = 10). We also analyzed the relationships between the expression of VHL and the clinical characteristics. Results The positive rate of VHL was significantly lower in HCC tissues(45%)than that in paired nocancerous tissue(82. 5%; χ~2= 12. 17,P <0. 0001) and normal hepatic tissues(90%,P = 0. 014). The positive rate of VHL was significantly lower in the vascular invasion groups.The cumulative survival rates at 1,3,5 year VHL positive expression group and VHL negative expression group were 83. 3%,61. 1%,55. 5% and 59. 1%,50%,40. 9%,respectively. Conclusions The expression of VHL are closely related with the biological behavior and prognosis of HCC.
引文
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