X染色体连锁凋亡抑制蛋白在宫颈鳞癌组织中的表达及其与新辅助化疗耐药的关系

详细信息    查看全文 | 推荐本文 |

英文篇名：The relationship between the expression of X-linked inhibitor of apoptosis protein in cervical squamous cancer and neoadjuvant chemotherapy resistance 作者：陈为 ; 王善林 ; 周龙书 ; 陈晓东 ; 彭萍 英文作者：CHEN Wei1,WANG Shan-lin1,ZHOU Long-shu2,et al(1.Department of Gynecology and Obstetrics,General Hospital of Guangzhou Military Command of PLA,Guangzhou 510010;2.Department of Gynecology,The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China) 关键词：宫颈鳞癌 ; 新辅助化疗 ; X染色体连锁凋亡抑制蛋白 英文关键词：Cervical squamous cancer;Neoadjuvant chemotherapy;X-linked inhibitor of apoptosis protein 中文刊名：ZGZK 英文刊名：Chinese Journal of Clinical Oncology and Rehabilitation 机构：广州军区广州总医院妇产科;广州医学院第二附属医院妇科;广州军区广州总医院病理科; 出版日期：2013-07-20 出版单位：中国肿瘤临床与康复 年：2013 期：v.20 基金：广东省医学科学技术研究基金[编号:A2011446] 语种：中文; 页：ZGZK201307008 页数：3 CN：07 ISSN：11-3494/R 分类号：21-23

摘要

目的探讨宫颈鳞癌组织中X染色体连锁凋亡抑制蛋白(XIAP)的表达水平与新辅助化疗耐药是否相关。方法收集78例宫颈鳞癌患者的癌组织及40例正常宫颈及慢性宫颈炎组织,其中32例宫颈癌患者术前进行了新辅助化疗2个疗程,同时也收集2个疗程后的癌组织。根据疗效将32例患者分为化疗敏感组和耐药组。通过免疫组化SP法检测XIAP在宫颈癌组织、癌旁组织和新辅助化疗后的癌组织中的表达情况。结果相对于癌旁组织或正常组织中的低表达或几乎不表达,XIAP在癌组织中高表达,XIAP在癌组织中的表达与有无淋巴结转移和组织分化程度方面差异有统计学意义(P<0.05),在临床分期及年龄方面差异无统计学意义(P>0.05)。化疗敏感组中XIAP的表达积分化疗后较化疗前显著降低(P<0.01),而在化疗耐药组中差异无统计学意义(P>0.05)。化疗前两组患者的XIAP表达积分差异无统计学意义(P>0.05)。结论 XIAP在子宫颈癌组织中表达水平明显升高,可能与宫颈癌复发及转移有关。同时XIAP表达水平的变化与新辅助化疗是否耐药密切相关,可能为开发新的化疗药物提供靶位点。
        Objective To investigate the expression of the X-linked inhibitor of apoptosis protein(XIAP) in cervical squamous cancer and its association with clinical data and neoadjuvant chemotherapy resistance.Methods Total samplesfrom 78 patients including squamous carcinoma tissues and 40 controls from normal cervical tissues and cervicitis tissues were collected.There were 32 patients among the total patients treated with neoadjuvant chemotherapy for two courses.Samples were collected after chemotherapy.And the 32 samples were divided into two groups(sensitive group and resistant group) according the therapeutic effect.The expression of XIAP was detected in these samples by SP immuno-histochemical technique.Results The expression of XIAP protein in normal cervical tissues was negative or lower while expression was positive or higher.The positive rate of XIAP protein expression in cervical carcinoma tissue increased with the decline of cancer cells differentiation degree(P<0.05) and the lymphatic metastasis(P<0.05).There was no correlation between XIAP protein expression and age,clinical staging(P>0.05).The expression of XIAP after the treatment with neoadjuvant chemotherapy in the sensitive group is lower than the resistant group.There was no statistical significance in XIAP expression between the two groups before neoadjuvant chemotherapy.Conclusions Abnormal expression of XIAP may contribute not only to the recurrence and metastasis but also chemotherapy resistance of the squamous carcinoma of cervix.It is possible that XIAP will be a new target in the chemotherapy.

引文

[1]Rajcan-Separovic E,Liston P,Lefebvre C,et al.Assignmentof human inhibitor of apop tosis p rotein(IAP)genes XIAP,hiap1,and hiap2 to chromosomes Xq25 and 11q22-q23 by flu-orescence in situ hybridization[J].Genomics,1996,37:404-406.
    [2]Sapi E,Alvero AB,Chen W,et al,Resistance of ovarian carci-noma cells to docetaxel is XIAP dependent and reversible byphenoxodiol[J].Oncol Res,2004,14:567-78.
    [3]程晓东,吕卫国,叶枫,等.局部晚期子宫颈癌新辅助化疗价值的评估[J].中华妇产科杂志,2006,41:95-98.
    [4]菅志远,李宜雄,李小刚,等.X染色体连锁的凋亡抑制蛋白在胰腺癌组织及细胞中的表达及与化疗耐药的关系[J].中华消化杂志,2006,26:76-79.
    [5]Danson S,Dean E,Dive C,et al.IAPs as a target for antican-cer therapy[J].Curr Cancer Drug Targets,2007,7:785-794.
    [6]Kunze D,Kraemer K,Erdmann K,et al.Simultaneous siRNA-mediated knockdown of antiapoptotic BCL2,Bcl-xL,XIAP andsurvivin in bladder cancer cells[J].Int J Oncol,2012,41:1271-1277.