摘要
为了研究羟基磷灰石(HA)纳米微粒对不同种类低温保护剂(质量浓度为60%的渗透性丙三醇和质量浓度为50%的非渗透性PEG-600)反玻璃化结晶的影响,利用低温显微镜研究了含有不同粒径(20nm、40nm、60nm)和不同质量浓度(0.1%、0.5%)HA纳米微粒的丙三醇和PEG-600溶液在反玻璃化过程中的结晶现象。实验结果表明:丙三醇和PEG-600溶液在反玻璃化过程中的冰晶形貌分别为枝状和球形,且形貌都不随添加纳米微粒而改变。与未添加纳米微粒的丙三醇溶液相比,添加60nm、0.5%纳米微粒溶液的析晶分数仅为未添加溶液的1/7。PEG-600溶液在-64℃~-52℃区间内的析晶分数显著增加,其中添加40nm、0.5%纳米微粒的PEG-600溶液的析晶分数增加了90%。冰晶的生长速率存在两个极大值现象,而添加40nm、0.5%纳米微粒的丙三醇溶液、未添加和添加60nm纳米微粒的PEG-600溶液的冰晶生长速率都只有一个极大值。
To study the effects of Hydroxyapatite(HA) nanoparticles on devitrification of different cryoprotectant solutions,the crystallization of Glycerol(mass concentration is 60%) and PEG-600(mass concentration is 50%) solutions with HA nanoparticles of different particle size(20nm、40nm、60nm) and concentrations(0.1% 、0.5%) were observed under a cryomicroscope.Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively,and both structures are not changed by adding nanoparticles.The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1 /7 of that of Glycerol solution without nanoparticles.The ice fraction of PEG-600 solution increased drastically between-64℃ and-52℃,and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%.Ice growth rate has two maximum normally,however,there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm,PEG-600 solution with the particle size of 60nm,and PEG-600 solution without nanoparticles.
引文
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