摘要
可溶性膳食纤维(SDF)具有良好的离子吸附性能,酪蛋白磷酸肽(CPP)具有促进钙吸收能力,基于此本文制备了一种高分子聚合物的钙离子传递载体,以获得优良的促进人体钙吸收效果。在pH4.6条件下,采用胰蛋白酶和钙-乙醇沉淀法水解SDF与酪蛋白美拉德反应产物,获得SDF-CPP共聚物。利用差示扫描量热法(DSC)、扫描电子显微镜(SEM)、傅里叶红外(FTIR)、X射线衍射(XRD)、体外试验检测共聚物的形成、构象变化与体外促进钙吸收能力。研究结果表明,SDF通过静电作用力吸附CPP分子,分子间β-折叠含量增加形成交联共聚物,衍射峰强度增加,结晶度增大使得共聚物热稳定性显著提高。制得的共聚物在pH 3.0-9.0范围内能够完全溶解,在pH 5.6和pH 7.4时,共聚物持钙能力分别为10.68%和13.81%,相比单体分别提高了2.88%和4.48%。在pH 7.4以及浓度为50mg/L时,共聚物能够较好地延长磷酸钙沉淀时间。本研究将为新型共聚物基础研究以及工业化生产提供参考依据。
Soluble dietary fibers(SDF) showed a strong adsorption capacity for cations.Casein phosphopeptide(CPP) could promote the calcium absorption.Based on this,a high copolymer carrier for calcium ions was prepared to increase calcium utilization of human body.This study established a process to produce tryptic SDF-CPP copolymer carrier from Maillard products through precipitated with Ca/ethanol at pH4.6.The analysis of differential scanning calorimetry(DSC),scanning electron microscopy(SEM),fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD) and in vitro experiment provided novel informations about the formation,conformational changes and calcium binding capacity of copolymer.The results showed that the combination of electrostatic interaction governing the adsorption between SDF and CPP molecular.The crosslinking between SDF and CPP was due to the increasing intermolecular(J-sheet formation.The increased crystallinity of copolymer significantly improved its thermal stability.The copolymer was found to be completely soluble in range of pH from 3.0 to9.0.The copolymer could bind 13.81%calcium at pH7.4 and 10.68%at pH5.6.Compared to the control,calcium binding capacity significantly increased in the copolymer.Under the condition of pH7.4 and the concentration of 50 mg/L,the calcium phosphate deposition time of copolymer could be prolonged than control.The information achieved from this report will be essentially valuable in the rational manufacture of SDF-CPP for basic research and industrial applications as an alternative to conventional copolymers.
引文
