摘要
Three dimensional(3D) aligned electrospun fibers hold a promising potential in a wide range of biomedical areas, including bio-sensors, controlled drug release and tissue engineering. Due to certain inherent technical challenges involved in fabrication, there has been a growing need for the fabrication of these fibers with tunable microarchitectures, porosity and overall morphology. Thus, it is highly desired to develop novel, cost-effective and easy to scale-up fabrication for 3D aligned electrospun fibers, especially in the purview of their usage in biomedical applications. Herein, we have developed a facile preparation of 3D PVDF aligned fibers(3D AFs) by an improved electrospinning technique. The obtained 3D AFs showed enhanced controllability on morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed an excellent in vitro biocompatibility, by facilitating adhesion and growth of human mesenchymal stem cells(h MSCs) within the 3D nanofiber micro-architectures. 3D AFs with aligned morphology enhanced the cellular activities and induced directional cell growth along the direction of nanofiber orientation, thereby providing an excellent cue for the anchorage and migration dependent h MSCs. More importantly, cell proliferation on the 3D AFs was found to be significantly higher than that on the nanofiber mats(NFMs). Combined with controllable morphology and structure, we anticipate that this finding greatly enhances great potential applications of electrospun 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.
Three dimensional(3D) aligned electrospun fibers hold a promising potential in a wide range of biomedical areas, including bio-sensors, controlled drug release and tissue engineering. Due to certain inherent technical challenges involved in fabrication, there has been a growing need for the fabrication of these fibers with tunable microarchitectures, porosity and overall morphology. Thus, it is highly desired to develop novel, cost-effective and easy to scale-up fabrication for 3D aligned electrospun fibers, especially in the purview of their usage in biomedical applications. Herein, we have developed a facile preparation of 3D PVDF aligned fibers(3D AFs) by an improved electrospinning technique. The obtained 3D AFs showed enhanced controllability on morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed an excellent in vitro biocompatibility, by facilitating adhesion and growth of human mesenchymal stem cells(h MSCs) within the 3D nanofiber micro-architectures. 3D AFs with aligned morphology enhanced the cellular activities and induced directional cell growth along the direction of nanofiber orientation, thereby providing an excellent cue for the anchorage and migration dependent h MSCs. More importantly, cell proliferation on the 3D AFs was found to be significantly higher than that on the nanofiber mats(NFMs). Combined with controllable morphology and structure, we anticipate that this finding greatly enhances great potential applications of electrospun 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.
引文
