摘要
利用PCR技术从犬贾第虫滋养中扩增出了2004bp大小的MM/Sac-C/1基因,构建了p MD-MM/Sac-C/1克隆载体,并进行了序列测定,利用计算机对该序列进行了核苷酸同源性比较。结果表明,与Gene Bank登陆的蓝氏贾第虫WB株AF236019序列比较,其同源性为98%,蛋白质同源性比较结果显示,与蓝氏贾第虫WB株表面抗原的同源性为90%,获得了犬贾第虫MM/Sac-C/1基因。
Cloning and sequencing of MM/Sac-C/1 gene in Giardia canis The gene was amplified by PCR,the cloning vector p MD-MM/Sac-C/1 was constructed and sequenced.The homology of MM/Sac-C/1 gene to AF236019 in Gene Bank was 98% in the nucleotide,and 90% in the amino acid levels.It was demonstrated that Giardia canis trophonts have MM/Sac-C/1 gene.
引文
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