摘要
Sisal zebra disease is a devastating oomycetes disease,one of most serious diseases of sisal hemp.There are 12 samples of sisal zebra disease collected from major production areas of Hainan,Guangdong and Guangxi,from which genomic DNA of its pathogens are extracted,and the rDNA-ITS sequence amplification is conducted.The result of sequence comparison is consistent with the morphological identification results.This suggests that all these tested strains of pathogens are Phytophthora nicotianae Breda.Results of phylogenetic tree which is constructed under N-j method show that,12 tested strains can be divided into two groups and they are of certain geographical differentiation.The above research will lay foundations for future researches in aspects of occurrence regularity,control methods and mechanisms of resistance of agave zebra disease and provide them with theoretical basis.
Sisal zebra disease is a devastating oomycetes disease,one of most serious diseases of sisal hemp.There are 12 samples of sisal zebra disease collected from major production areas of Hainan,Guangdong and Guangxi,from which genomic DNA of its pathogens are extracted,and the rDNA-ITS sequence amplification is conducted.The result of sequence comparison is consistent with the morphological identification results.This suggests that all these tested strains of pathogens are Phytophthora nicotianae Breda.Results of phylogenetic tree which is constructed under N-j method show that,12 tested strains can be divided into two groups and they are of certain geographical differentiation.The above research will lay foundations for future researches in aspects of occurrence regularity,control methods and mechanisms of resistance of agave zebra disease and provide them with theoretical basis.
引文
