摘要
药物分离分析技术是新药发现与创制中一项重要技术。药物代谢动力学、中药物质基础与活性成分分析以及组合生物合成与新药研发等均面临着分析对象体系复杂、实际样品分析中基质干扰、分离介质分子识别选择性差、分析目标物含量低微乃至低于先端仪器检测灵敏度等瓶颈问题,同时也给分析科学家提出了研究高效、微量、灵敏、精准分析新方法的机遇与挑战。近年来,本课题组以重点解决药物分析中复杂体系的分离分析中的瓶颈问题,发展高效、微量、灵敏、精准药物分析方法学为目标,开展了基于仿生聚多巴胺修饰技术的新型管内固相微萃取及Jacket-free搅拌棒固相萃取技术[1-5]、基于金属有机骨架材料及石墨烯为特征分子识别固定相的毛细管电色谱柱技术[6-8]及其在药物分析中应用研究,本报告拟介绍本课题组在上述方向取得的部分最新进展与成果[1-8]。
The separation techniques for pharmaceutical analysis are important in the drug discovery.In fact,there are several bottle-neck analytical problems which affect and slow down the progress in research and development of new drugs,such as the complicated real sample matrix,trace content of target ananlytes which is even lower than the limitation of detection of state-of- the-art analytical instruments,low separation selectivity and efficiency of separation media.These problems are challenging and demanded for analytical scientists to solve.In recent years,our group is focused on the development of novel solid-phase microextraction and capillary electrochromatographic column technology for solving these problems in pharmaceutical analysis.This talk will introduce recent advances in our group,including in-tube solid-phase microextraction and jacket-free stir bar sorptive extraction based on mussel-inspired polydopamine functionalization[1-5],and capillary electrochromatographic column technology based on the novel stationary phases of metal organic frames and grapheme materials for pharmaceutical analysis[6-8].
引文
1.J.Zhang,W.Zhang,T.Bao,Z.Chen*,Polydopamine-based immobilization of zeolitic imidazolate framework-8 for in-tube solid-phase microextraction,J.Chromatogr.A,2015,1388,9-16
2.Z.Zhang,W.Zhang,Z.Chen*,Jacket-free stir bar sorptive extraction with bio-inspired polydopamine-functionalized immobilization of cross-linked polymer on stainless steel wire,J.Chroamtogr.A,2015,1407:1-10
3.W.Zhang,Z.Zhang,J.Zhang,J.Meng,T.Bao,Z.Chen*,Covalent immobilization of graphene onto stainless steel wire for jacket-free stir bar sorptive extraction,J.Chromatogr.A,2014,1351:12-20
4.W.Zhang,J.Zhang,T.Bao,W.Zhou,J.Meng,Z.Chen*,Universal multilayer assemblies of graphene in chemically resistant micro-tubes for microextraction,Anal.Chem.2013,85:6845-6854
5.W.Zhang,Z.Chen*,Mussel inspired polydopamine functionalized poly(ether ether ketone)tube for online solid-phase microextraction-high performance liquid chromatography and its application in analysis of protoberberine alkaloids in rat plasma,J.Chromatogr.A,2013,1278:29-36
6.T.Bao,J.Zhang,W.Zhang,Z.Chen*,Growth of metal-organic framework HKUST-1 in capillary using liquid-phase epitaxy for open-tubular capillary electrochromatography and capillary liquid chromatography,J.Chromatogr.A,2015,1381:239-246
7.J.Zhang,W.Zhang,T.Bao,Z.Chen*,Enhancement of capillary electrochromatographic separation performance by conductive polymer in a layer-by-layer fabricated graphene stationary phase,J.Chromatogr.A,2014,1339:192-199
8.J.Zhang,W.Zhang,T.Bao,Z.Chen*,Mussel-inspired polydopamine-assisted hydroxyapatite as stationary phase for capillary electrochromatography,Analyst,2014,139:242-250
