摘要
目的：碘化油栓塞肿瘤血管能引起残余肿瘤细胞内血管内皮生长因子VEGF蛋白的表达增强；本实验采用姜黄素联合栓塞方法,实验观察姜黄素联合肝动脉栓塞兔VX-2肝移植瘤对肿瘤的生长及VEGF蛋白的影响；探讨姜黄素能否抑制肿瘤的生长并能否抑制碘化油栓塞引起的残余肿瘤细胞内VEGF蛋白的表达增强。
方法：40只新西兰大白兔接种VX-2瘤块复制肝癌模型,经肝动脉灌注栓塞后有36只达到要求合格,将这36只实验兔随机分成4组。A组：肝素水组(模型组)；B组：姜黄素灌注组；C组：碘化油栓塞组；D组：姜黄素碘化油乳剂栓塞组。治疗前和治疗后2周分别用MRI测量肿瘤直径,计算肿瘤体积和体积变化,治疗后2周处死实验兔,取出标本,采用免疫组化和Western印迹测定残余肿瘤中VEGF蛋白的表达。
结果：
1、肿瘤体积(cm3)变化：A组18.30±0.61；B组：18.36±1.11；C组：6.06±1.13；D组：5.97±0.92。两两之间比较：B组与A组比较：差异无显著性,P值：0.88(P>0.05);C组与A组比较：差异有显著性,P值<0.01(P<0.05);D组与A组比较,差异有显著性,P值<0.010(P<0.05)；C组与B组比较：差异有显著性,P值<0.01(P<0.05);D组与B组比较：差异有显著性,P值<0.01(P<0.05);D组与C组比较：差异无显著性,P值0.831(P>0.05)。
2、免疫组化VEGF蛋白的平均光密度值：A组：0.599±0.182；B组：0.545±0.284;C组：0.867±0.210；D组：0.603±0.186。两两之间比较：B组与A组比较,P值为0.46(P>0.05),差异无显著性；C组与A组比较,P值<0.01(P<0.05),差异有显著性；D组与A组比较,P值0.952(P>0.05),差异无显著性；C组与B组比较,P值<0.01(P<0.05),差异有显著性；D组与B组比较,P值0.424(P>0.05),差异无显著性；D组与C组比较,P值0.01(P<0.05),差异有显著性。
3、Western印迹杂交测定VEGF蛋白表达水平：对照组(A组)：46.17±13.44;B组：47.57±15.02；C组：82.56±18.85；D组：53.84±16.55。两两之间比较：B组与A组比较,P值为0.795(P>0.05),差异无显著性；C组与A组比较,P值<0.01(P<0.05),差异有显著性；D组与A组比较,P值0.157(P>0.05),差异无显著性；C组与B组比较,P值<0.01(P<0.05),差异有显著性；D组与B组比较,P值0.242(P>0.05),差异无显著性；D组与C组比较,P值<0.01(P<0.05),差异有显著性。
结论：
1、单用姜黄素不能抑制肿瘤的生长,也不能有效抑制残余肿瘤细胞内VEGF蛋白的表达。
2、姜黄素联合栓塞兔VX-2肝移植瘤能够有效抑制碘化油栓塞引起残余肿瘤细胞内VEGF蛋白的表达增高,但未见明显其抑瘤效应。
Objective:
The way of tumor vessels embolismed by iodinate oil can enhance the express of vascular endothelial growth factor (VEGF) protein in residual tumor cell;the experient associated curcumin with embolizer in order to observe the effect on the expression of tumor growth and VEGF in rabbit VX-2 liver tumor transcatheter arterial embolization(TAE) combining curcumin; probing the curcumin was or was not able to inhabit the growth of tumor and the enhancement of VEGFprotein in residual tumor brought out by iodinate oil。
Methods:
VX-2 carcinoma pieces were surgically implanted into the left liver lopes of 40 New Zealand white rabbitsin order to build the model of liver cancer,those of thirty-six were successfully transcathetered arterial embolization.those were randomly divided into the four groups,with nine rabbits in each group. Controlgroup:treated with heparin saline; B group:treated with hepatic arterial infusion therapy combining curcumin;C group:Hepatic artery embolism combining iodized oil; D group:treated with transcatheter arterial emboli-zation combining curcumin and iodized oil. Two weeks after embolism, the rabbits were sacrificed and the tumors were taken out. The volume of tumor and the rate of tumor grate was calculated before and after TAE. Immunohistochemistry and Western blotting were used to detect expression of vascular endothelial growth factor (VEGF) protein。
Results:
1. Variation of tumor volume A group:18.30±0.61; B group:18.36±1.11; C group:6.06±1.13;D group:5.96±0.92.draw a parallel between the two:the volume of B group was not supressed and comparedwith the volume of A group,it had not obvious differences(P=0.88);the volume of C group was supressed and comparedwith the volume of A group, it had obvious differences(P<0.01); the volume of D group was not supressed and comparedwith the volume of A group, it had obvious differences(P=0.175); the volume of C group was supressed and comparedwith the volume of B group, it had obvious differences(P<0.01); the volume of D group was supressed and comparedwith the volume of B group, it had obvious differences (P<0.01); the volume of D group was not supressed and comparedwith the volume of C group, it had not obvious differences(P=0.831).
2.VEGF protein was detected by Immunohistochemistry as follow:A group 0.599±0.182, B group:0.545±0.284, C group:0.867±0.210, D group:0.603±0.186; draw a parallel between the two:the volume of B group was not supressed and comparedwith the volume of A group,it had not obvious differences(P=0.46);the volume of C group was supressed and comparedwith the volume of A group, it had obvious differences(P<0.01); the volume of D group was not supressed and comparedwith the volume of A group, it had obvious differences(P=0.592); the volume of C group was supressed and comparedwith the volume of B group, it had obvious differences(P<0.01); the volume of D group was supressed and comparedwith the volume of B group, it had obvious differences(P:0.242); the volume of D group was not supressed and comparedwith the volume of C group, it had not obvious differences(P=0.01).The trend of VEGF protein expression was as the same as that of VEGF he relative light intensity
Conclusions:
1.Sing-use curcumin neither inhibit effectively the grate of tumor,nordepress the expression of VEGF protein inresidual tumor cell.
2.curcumin epress the enhancement expression of VEGF protein inresidual tumor cell brought out by iodinate oil.,but curcumin can not obviously inhibit the grate of tumor.
引文
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