齐口裂腹鱼营养学研究
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摘要
本文进行了齐口裂腹鱼营养学方面的研究。分析了齐口裂腹鱼三个生长阶段肌肉的营养成分,并对其营养价值进行了评价;研究了幼鱼对蛋白质、脂肪、无机盐和能量的需求;研究了齐口裂腹鱼对饲料蛋白质的离体消化率和酶解速率。现将主要结果总结如下:
     1.齐口裂腹鱼肌肉营养成分分析及营养价值评价
     采用国际标准方法对齐口裂腹鱼小规格幼鱼(1.9±0.2g)、大规格幼鱼(72.4±7.3g)和成鱼(501.8±35.5g)肌肉中的水分、蛋白质、脂肪含量及脂肪酸、氨基酸的组成进行了测定分析,并对其营养价值进行了评价。结果表明:
     (1)不同生长阶段的齐口裂腹鱼肌肉常规营养成分基本相同;齐口裂腹鱼肌肉中水分含量最高,蛋白质、脂肪、灰分依次降低,无氮浸出物最低。水分、蛋白质、灰分变动较小,而脂肪和无氮浸出物变动较大。
     (2)齐口裂腹鱼肌肉中主要含有27种脂肪酸,其中饱和脂肪酸(SFA)9种,不饱和脂肪酸(UFA)18种;齐口裂腹鱼肌肉脂肪酸的组成有三个特点:不饱和脂肪酸的含量高于饱和脂肪酸;不饱和脂肪酸中高度不饱和脂肪酸的含量较高,平均含量为19.88%,;从小规格幼鱼至成鱼,不饱和脂肪酸含量变化很小,但高度不饱和脂肪酸含量有很大差异,特别是大规格幼鱼与成鱼之间发生了显著变化(P<0.05)。
     (3)齐口裂腹鱼肌肉中蛋白质由18种氨基酸组成,各种氨基酸在不同生长阶段肌肉中的含量比较稳定,人体所必需的氨基酸占氨基酸总量的47.93%,谷氨酸的含量很高,为氨基酸总量的14.56%;鲜味氨基酸的含量为29.44[g.(100g)~(-1)干重],齐口裂腹鱼肌肉氨基酸评分和化学评分分别为70和41。
     2.齐口裂腹鱼幼鱼饲料营养素适宜含量的研究
     设计9个不同蛋白质水平(24.09%,27.96%,31.89%,36.11%,40.15%,44.07%,47.92%,52.07%,56.17%)的饲料,对齐口裂腹鱼幼鱼(1.97±0.28g)进行了50d的生长试验。结果显示:随饲料蛋白质含量的升高,增重率、饲料系数、蛋白质效率均产生了显著的差异(P<0.05),饲料蛋白质水平从24.09%增至40.15%时,鱼体的增重率表现为线形上升,在一定范围内(X<40.15%)幼鱼增重率(Y)与饲料蛋白质含量(X)的回归方程为:Y=3.0827X-33.362(r=0.9862,P<0.01)。在整个蛋白质含量梯度试验
    
     西南农业大学硕士学位论文
    ,...............
    范围内,幼鱼增重率与饲料蛋白质含量呈二次曲线关系,按抛物线拟合得方程:
    Y二一163.2+n.825X一0.1427X2(r二0.9207)。以回归分析法求得齐口裂腹鱼幼鱼饲料蛋白
    质适宜含量范围为35.96%一41.43%。
     用正交试验法Lg(34),以增重率、饲料系数和蛋白质效率为评价指标,筛选齐口裂
    腹鱼幼鱼(2 .14士0.319)最适生长的饲料营养素含量为:蛋白质40.06%、脂肪6.02%、
    无机盐4%、总能13.09 kJ.g一,。各营养素对幼鱼的生长及蛋白质效率的影响大小为:蛋
    白质>总能>脂肪>无机盐,其中蛋白质为影响生长和鱼体成分的主要因素(P<0 .05),
    饲料蛋白质水平对鱼体水分和脂肪含量有明显影响(P<0.05),其它营养素水平变化则
    对鱼体成分没有明显影响(P>0 .05)。
    3.齐口裂腹鱼对饲料蛋白质的离体消化率和酶解速率研究
     试验采用离体研究方法,以齐口裂腹鱼肠道、肝胰脏的粗酶液为酶源,测定了其对
    9种饲料(鱼粉、肉骨粉、羽毛粉、豆粕、菜粕、棉粕、玉米、次粉、酵母粉)膨化前
    后蛋白质的离体消化率及在4.Ohr内氨基酸生成量随反应时间的关系和氨基酸的生成速
    率。用氨基酸生成速率表示蛋白质酶解速率。结果表明:
     (1)齐口裂腹鱼肠道对同一饲料蛋白质的离体消化率和酶解速率高于肝胰脏;
     (2)膨化对饲料蛋白质的离体消化率和酶解速率有影响。鱼粉、肉骨粉、豆粕膨化后
    离体消化率和酶解速率下降;羽毛粉、菜粕、玉米、次粉膨化后离体消化率和酶解速率
    上升,特别是玉米尤为明显;棉粕、酵母粉膨化前后离体消化率和酶解速率变化不显著
     (P(0 .05);
     (3)不同饲料间粗蛋白质消化率、酶解速率有差异,对非膨化饲料,鱼粉>豆粕夕肉骨
    粉>菜粕>酵母粉>棉粕>羽毛粉>次粉>玉米;对膨化饲料,膨化鱼粉>膨化菜粕>膨化羽毛
    粉>膨化肉骨粉>膨化玉米>膨化棉粕>膨化酵母粉>膨化次粉>膨化豆粕。
The research is on nutrition of Schizothorax Prenanti. Nutrient contents of the muscle of Schizothorax prenanti at three different stages were analysised and its nutrient value was evaluated. The nutritional requirement of juvenile Schizothorax Prenanti for protein,fat, minerals and gross energy were studied. And so did the digestibility and enzymolysis rate in vitro of Schizothorax Prenanti to different feed.
    Experiment One: Evaluation of nutritive value and analysis of the nutritive composition in the muscle of Schizothorax Prenanti.
    Contents of the common nutritive composition were analyzed by national standard method in the muscle of small juvenile Schizothorax Prenanti ( average weight: 1.9±0.2g) , large juvenile fish (average weight: 72.4±7.3g) and adult fish ( average weight : 501.8± 35.5g ) , and so was the composition of fatty acids , amino acids . The results were as following:
    (1)In the muscle of Schizothorax Prenanti at three different stages, the common nutritive compositions were no significant difference, the moisture was the highest, among protein, fat and ash, the content descended in rum, and the NFE was the least, the contents of moisture, protein and ash had no remarkable variety, but fat and NFE had.
    (2)There were 27 kinds fatty acids in the muscle of Schizothorax Prenanti, include 9 kinds SFA and 18 kinds UFA. The composition of fatty acids had three characteristics. First, the content of UFA was higher than that of SFA. Second, the UFA contained more HUFA, the average proportion of HUFA in fatty acids was 19.88%. And third, from small juvenile fish to adult fish the contents of UFA ranged were very little, but the contents of HUFA had evident variety, especially, there were notable difference between large juvenile fish and adult fish (P<0.05).
    (3)There were 18 kinds amino acids were examined from the muscle of Schizothorax Prenanti. At different stages, the contents of all amino acids in the muscle were steady. The essential amino acids for human being accounted for 47.93% of total amino acids. The content of glutamic acid was considerably high, reaching about 14.56% of total amino acids . The content of delicious amino acids was 29.44g/100g dry matter. The AAS of the muscle was 70 and CS was 41.
    Experiment Two: Studies on suitable nutrient content in formulated diet for juvenile Schizothorax Prenanti
    The nutritional requirement of juvenile Schizothorax Prenanti for protein,fat, minerals and gross energy were studied through 2 expriments.
    A 50-day growth experiment was conducted to determine the optimum dietary protein level for juveniles of Schizothorax Prenanti, 9 protein levels (24.09%, 27.96%, 31.89%,
    
    
    
    36.11%, 40.15%, 44.07%, 47.92%, 52.07%, 56.17%) were formulated .The results showed that dietary protein levels influenced significantly the growth rate, feed coefficient, protein efficiency(P<0.05), when protein level ranged from 24.09% to 40.15%, the growth rate ascended linearly. In a definite range(CP<40.15%),the regression equation between growth rate(Y) and dietary protein level(X) was Y=3.0827X-33.362(r=0.9862, P<0.01). Among all the protein levels, the regression equation between growth rate(Y) and dietary protein level(X) was Y=-l63.2+11.825X-0.1427X2(r=0.9207). It was suggested that the optimum dietary protein level for the juveniles of Schizothorax Prenanti should be 35.96%-41.43%.
    An orthogonal designL9(34) was adopted to determine the nutritional requirement for protein (40.42%),fat (6.02%), minerals(4.0%),and gross energy(13.09 kJ.g-1),respectively, using growth rate, protein efficiency and feed conversion ratio as the biological indexes to assess the feeding effect. The results showed that the influence of nutrients on the growth rate and protein efficiency was as following order: protein> gross energy > fat > minerals, in the range of the factor levels. Thus, protein level had a main effect on growth and body composition of Schizothorax Prenanti (P<0.05) .
    Experiment Three: Studies on digestibility in vitro and enzymolysis rate of Schizothorax
引文
[1]丁瑞华(主编).四川鱼类志[M].成都:四川科学技术出版社,1994:370-371
    [2]杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993
    [3]尾崎久雄.鱼类消化生理(上、下)(李爱杰译)[M].上海:上海科技出版社,1985.
    [4]陆忠康(主编).简明中国水产养殖百科全书[M].上海:上海科技出版社,1985.
    [5]方静,潘康成.齐口裂腹鱼肌肉营养成分分析[J].水产科学,2002,21(1):17—18
    [6]梁银铨,崔希群,刘友亮.鳜肌肉生化成分分析和营养品质评价[J].水生生物学学报,1998,22(4):386-388
    [7]严安生,熊传喜,周志军,等.异育银鲫的含肉率及营养价值评价[J].水利渔业,1998,18(3):16-19
    [8]洪瑞川,李思光,余扬帆.万安玻璃红鲤的肌肉营养成分分析[J].水生生物学学报,1997,21(2):109-113
    [9]张忠萍,余志坚,肖增雪,等.黄尾密鲴肌肉营养成分分析.水利渔业,2002,22(6):1-2
    [10]王道尊,刘玉芳.青鱼、草鱼、团头鲂及其天然饲料生化组成的分析[J].水产科技情报,1987,(4):11-16
    [11]陈定福,何学福,周启贵.南方大口鲶和鲶鱼含肉率及鱼肉的营养成分[J].动物学杂志,1990,25 (1):7-9
    [12]张銮光.鲤、鲫鱼肌肉水解氨基酸和游离氨基酸的初步研究[J].水生生物学报,1988,12(2):181-185
    [13]中国预防医学科学院营养与食品卫生研究所编著.食物成分表(全国代表值)[M].北京:人民卫生出版社,1991.30-82,92-104
    [14]刘志皋.食品营养学[M].北京:中国轻工业出版社,1997
    [15]方静,周毅.齐口裂腹鱼肠粘膜上皮超微结构研究[J].四川农业大学学报,1995,13(2):218—220,258
    [16]潘康成,方静.齐口裂腹鱼端脑形态和组织学研究[J].四川农业大学学报,2000,20(2):144—147
    [17]方静,周毅.齐口裂腹鱼和重口裂腹鱼消化道形态和组织结构的观察[J].四川农业大学学报,1995,13(1):101—106
    [18]方静,周毅,谢林.齐口裂腹鱼口咽腔粘膜上皮及味蕾的超微结构研究[J].四川农业大学学报,1996,14 (2):297—300,236
    [19]方静,谢林,李逊,等.齐口裂腹鱼消化道粘膜上皮的扫描电镜观察[J].水生生物学学报,1995,19 (2):188—189
    [20]江伟殉,刘毅(主编).营养与食品卫生学[M].北京:北京医科大学和中国协和医科大学联合出版社,1992.4-14
    [21]赵法伋,郭俊生,陈洪章,等.大豆平衡氨基酸营养价值的研究[J].营养学报,1986,8(2):50
    
    153-158
    [22]王道尊,汤峥嵘,谭玉均.中华鳖生化组成的分析Ⅰ.常规营养成分的含量及脂肪酸的组成[J].水生生物学报,1997,21(4):299-304
    [23]闻芝梅,陈君石(主译).现代营养学(第七版)[M].北京:人民卫生出版社,1998.57-63
    [24]谢刚,杨红波,邢慧铃,等.鳗鲡肌肉生化成分的分析[J].淡水渔业,1989,(4):6-9
    [25]汤峥嵘,王道尊,谭玉均.中华鳖生化组成的分析Ⅲ.肌肉氨基酸的组成[J].水生生物学报,1998,22(4):307-313
    [26]温小波,库夭梅,李伟国.4种优质底栖淡水鱼类肌肉营养成分的比较[J].大连水产学院学报,2003,18(2):99-103
    [27]刘纯洁,张娟婷(编译).食品添加剂手册[M].北京:中国展望出版社,1988.157-160
    [28]苏秀榕,李太武,欧阳芬.三疣梭子蟹营养成分的研究[J].营养学报,1996,18(3):342-346
    [29]温安祥,曾静康,何涛.齐口裂腹鱼肌肉的营养成分分析[J].水利渔业,2003,23(1):13-15
    [30]赵振山,高贵琴,印杰,等.泥鳅和大副泥鳅营养成分分析[J].水利渔业,1999,19(3):16-17
    [31]陈琴,陈意明,蔡子德,等.三种野生江河鱼类的含肉率及营养成分的比较[J].渔业现代化,2001,3:7-9
    [32]宋苏祥,孙大江,范兆廷,等.虹鳟鱼肌肉营养成分的分析[J].大连水产学院学报,1996,11(1):70-73
    [33]蔡焰值.斑点叉尾鲴营养成分分析[J].水产科技,1998,(3):24-26
    [34]白利丹,周景祥,黄权,等.大眼鰤鲈肌肉营养成分分析[J].淡水渔业,2000,30(1):34-36
    [35]陈学存(主编).应用营养学[M].北京:人民卫生出版社,1984.8-14
    [36]谢宗墉,刘竹伞(主编).水产品的食疗与健康[M].北京:中国农业出版社,1995.1-18
    [37]王玉堂,熊贞(主编).淡水冷水性鱼类养殖新技术[M].北京:中国农业出版社,2001.1-3
    [38]吴遵霖.鱼类营养与配合饲料[M].北京:农业出版社,1990.38-45
    [39]冷向军、李小勤.水产动物营养与畜禽营养的比较[J].饲料研究,2002,(2):20-30
    [40]韩庆,夏维福,罗玉双,等.不同营养水平对黄颡鱼春片鱼种生长的影响[J].饲料工业,2002,23(7):43—44
    [41]张泽芸,陈先均,张季涛.长吻鮠营养需要量的研究[J].动物学研究,1993,14(2):150-157
    [42]曹俊明,关国强,刘永坚,等.饲料蛋白质、脂肪、碳水化合物水平对草鱼生长和组织营养成分组成的影响[J].水产科技情报,1997,24(2):56-59
    [43]陈建明,吴文,陈德富,等.淡水白鲳营养需要量的研究[J].浙江水产学院学报,15(2):117-120
    [44]杨代勤,陈芳,李道霞,等.黄鳝的营养素需要量及饲料最适能量蛋白比[J].水产学报,2002,24(3):259—262
    [45]钱国英,朱邱华.配饵中能量蛋白比与中华绒螯蟹生长之间的关系[J].中国水产科学,2000,7(1):55—59
    [46]袁春营,崔青曼.虹鳟营养需要量研究[J].中国饲料,2003,(17):21-22
    [47]任维美.虹鳟饲料中棉籽饼的适宜含量[J].饲料研究,2002,(1):37
    
    
    [48]肖懿哲.史氏鲟饲料中脂肪的最适含量[J].饲料研究,2002,(3):38
    [49]徐新章,李爱杰.中国对虾配饵中蛋白质、糖、纤维素、脂肪的适宜含量及日需要量研究.海洋科学,1988,6:1-6
    [50]陈兆祥(主编).渔业生物统计学[M].北京;中国农业出版社,1993.
    [51]张家国,王义强,邹师哲.不同蛋白质能量比饲料与夏花草鱼消化酶的关系[J]。上海水产大学学报,1997,6(1):54-58.
    [52]包吉墅,刘春,高晓莉,等.稚鳖的营养素需要量及饲料最适能量蛋白比[J].水产学报,1992,16(4):365-371.
    [53]杨国华.夏花青鱼饲料中最适蛋白质含量[J].水产学报,1981,5(1):49—55.
    [54]薛敏,李爱杰,董双林,等.中国对虾饲料中最佳蛋白能量比研究[J].青岛海洋大学学报,1998,28(2):245-252.
    [55]林鼎.鲩鱼鱼种生长阶段蛋白质最适需要量的研究[J].水生生物学集刊,1980,7(2):207—212.
    [56]王育锋,彭秀真,周嗣泉等.黄鳝全价配合饲料的研制[J].饲料研究,1996,(4):8—10.
    [57]廖翔华.鱼类营养需求研究进展[J].水生生物学报,1989,13(2):170—186.
    [58]戴祥庆.青鱼饲料最适能量蛋白比的研究[J].水产学报,1988,12(1):35—41.
    [59]吴江,张泽芸.大口鲶的营养需要[J].西南农业学报,1996,9(3):72-77
    [60]陈喜斌,庄平,曾翠平,等.中华鲟幼鲟蛋白质营养最适需要量[J].中国水产科学,2002,9(1):60-64
    [61]张文斌,谢小军,付世健,等.南方鲇的营养学研究:饲料的蛋白质水平[J].水生生物学报,2000,24(6):603—609
    [62]邓利,谢小军.南方鲇的营养学研究:人工饲料的消化率[J].水生生物学报,2000,24(4):347—355.
    [63]艾庆辉,谢小军.南方鲇的营养学研究:饲料中大豆蛋白水平对生长的影响[J].水生生物学报,2002,26(1):57—65.
    [64]钱国英.饵料中不同蛋白质、纤维素和脂肪水平对加洲鲈生长的影响[J].动物营养学报,2000,12(2):48—52.
    [65]中山大学生物系.生化技术导论[M].北京:科学出版社,1979:42-54
    [66]赵轶千.生理学实验指导[M].北京:人民卫生出版社,1985:111-112
    [67]林仕梅,罗莉,叶元土.鱼饲料消化率测定方法的研究进展[J].饲料研究,2000,(6):8—10.
    [68]罗莉,叶元土,林仕梅,等.异育银鲫肠道对膨化和非膨化饲料蛋白质的酶解速率研究[J].饲料工业,2003,24(9):40-43
    [69]王友慧,叶元土,林仕梅,等.3种鱼对鱼粉的酶解速率及体外消化率的研究[J].西南农业大学学报,2002,24(3):259—263
    [70]黄希国,方贤法,李加良.对鱼虾配合饲料制粒与膨化加工技术的分析[J].中国饲料,1998,(16):19—21
    [71]林仕梅.挤压膨化工艺在浮性水产饲料中的应用[J].粮食与饲料工业,2001,(3):15-18
    
    
    [72]林仕梅,罗莉,叶元土.膨化工艺对水产饲料营养价值的影响[J].饲料研究,2003,(11):29-33
    [73]王子淑.人体及细胞遗传学实验技术[M].成都:四川大学出版社,1987:1-40
    [74]林建彬,赵文祺,郑诚.不同加工条件对大豆饼(粕)品质的影响[J].饲料工业,1994,15(4):1-9
    [75]李素芬,杨丽杰,霍贵成.膨化处理对全脂大豆抗营养因子及营养价值的影响[J].畜牧兽医学报,2001,32(3):193-201
    [76]姜正安.挤压膨化工艺中原料的选择[J].中国饲料,2000,(19):22-23
    [77]王彩云.玉米膨化质构变化的研究[J].中国粮油学报,1998,13(3):41-43
    [78]Bromeley P J.Effects dietary protein,lipid and energy content on the growth of turbot(Scophthalmus maximus L.)[J]. Aquaculture,1980,19:359-369.
    [79]Halver E.Formulating Practical Diets for Fish[J].J Fish Res Bd Can,1976,33(4):1932—1939.
    [80]Press H,Oliva-Teles A.Influence of tempersture on protein utilization in juvenile European seabass.Aquaculture,1999,170:337—348 .
    [81]Garling,D.L,JR.and R.P.Wilson.The optimum dietary protein to energy ratio for channel catfish fingerlings,Ictalurus punctatus.J.Nutr, 1976,106:1368—1375.
    [82]Takeuchi,T.et al, Optimum ratio of energy to protein for carp.BJSSF,1979,45:983—987.
    [83]Sarbani D S.Studies of the digestive teacts and the digestive enzymes of the goldfish,Carassius auratus L.and largem the black bass,Micropterus salmoides(Lare'pech)[J].Biol.Bull,1951,244-257
    [84]Olatunde A A,Ogunbyi O A.Digestive enzymes in the alimentary tracts of three tropical catfish [J].Hydrobiologia,1977,56(1):21-24
    [85]Sugiura M,Ishikawa M,Sasaki M,etal.A new method for protease activity neasurement[J].Anal.Biochem,1979,97(10):11-15
    [86]Senger H.Digestive enzymes in larval Corgonus larvarentus L.J.Fish Bio1.1989,35:249-263
    [87]Takii K, Shimene S,Takeda M.Changes in digestive enzyme activities in eel affer feeding[J].Buil.jap.Soc.sci.Fish,1985,51 (12):2027-2031
    [88]Usy W,T Hecht.Assays on the digestive enzymes of sharptoon catfish,Clarias gariepinus(Pisces:Clariidae)[J].Aquaculture, 1987,83:301-313
    [89]Windell J T, Foltz J W, Sarokon J A.Methods of fecal collection and nurient leaching in digestibility studies[J].Prog Fish cult,1978,40:15-55
    [90]Akiyama,T.,S.Arai,T.Murai and T.Nose.Threonine,histidine and lysine requirements of chum salmon fry.Bull.Jpn.Soc.Sci.Fish.,51(4),1985,260-268
    [91]National Research Council,Nutrient requirement of coldwater fishes, No.16,National Academy of Sciences,1981
    [92]National Research Council,Nutrient requirement of warmwater fishes and shellfishes, No.16,National Academy of Sciences,1983
    [93]Lovell,T.,Nutrition and Feeding of Fish. Van Nostrand Reinhold,New York,1989
    
    
    [94]Saimt-Paul,U.,Potential for aquaculture of South American freshwater fishes:A Review[J]. Aquaculture,1996,54(3):211-241
    [95]Dabrowski.K, Protein requirements of grass carp(Ctenopharyngodon idella Val.)[J].Aquaculture,1977.12:63-73
    [96]Lall,S.P.,F.J.Bishop.Studies on the nutrient requirements of rainbow trout.Salmo gairdneri,grown in sea water and freshwater.Proc.FAO Technical Conference on Aquaculture.May, Kyoto.Japan. 1976,580-584
    [97]Mazid.M.A.et al.Growth response of Tilapia Zillii fingerlings fed isocaloric diets with variable protein levels[J].Aquaculture, 1979.18:112-115
    [98]Satpathy BB, Mukherjee D,Ray AK. Effects of dietary protein and lipid levels on growth, feed conversion and body composition in rohu, Labeo rohita (Hamilton), fingerlings[J]. International Journal of Dairy Technology,2003,9(1): 17-24
    [99]Nose.T..S.Arai.Optimum level of protein in purified diet for eel.Anguilla japonica.Bull.Freshwater Fish.Res.Lab. 1972,22:145-155
    [100]Ogino,C.Saito.K.,Protein nutrition in fish.1.The utilization of dietary protein by yong carp.Bull.Jap.Soc.Fish. 1970,36:250-254
    [101]Ogino,C. Protein nutritions of carp and rainbow trout.Bull.Jap.Soc.Sci.Fish. 1980.46:385-388
    [102]Moore B J.S S O Hung J,F Median.Protein requirement of hatch.ery-produced juvenile white sturgeon(Acipenser transmontanus)[J].Aquaculture,1988. 71:235-245
    [103]Kaushik S J,J Breque,D Blance.Requirement for protein and essential amino acids and their utilization by Siberian sturgeon(Acipenser baeri)[J].In:P Williot ed.Porch Sit Intern Sym On sturgeon.3-6 october, 1989.Berdeaux-France:CEMAGREF, 1991.25-39
    [104]Hung S S O.Nutrition and feeding of hatchery produced juvenile white sturgeon (Acipenser transmontanus),An overview.In:P.Wallet ed.Droc Ist intern Sys On sturgeon.3-6 october,1989.Berdeaux-France:CEMAGREF,1991a.65-77
    [105]Finn-Aikins F K,S S S Hung,et al.Growth,lipogensis and liver composition of juvenile white sturgeon (Acipenser transmontanus) fen different levels of D-glucose[J].Aquaculture,1992,105:61-72
    [106]Daniels W H,Robinson E H.Protein and energy requirement of juvenile red drum(Sciacnops ocdlatus)[J].Aquaculture,1986, 73:243-252
    [107]De Silva S S,Perera P A B.Studies on the young grey muller, Mugil cephalus L.I.Effects of salinity on food intake,growth and food conversion[J].Aquaculture,1976,(7):327-338
    [108]El-Sayed A M.Teshima S.Protein and energy requirements of Nile tilapia,Oreochromis niloticus,fry[J].Aquaculture, 1992,103:55-63
    [109]Nour A E A,Mabrouk H,Omar E,et al.Effect of feeding levels and stocking densities on growth performance and utilization of grey mullet(Mugil cephalus)[J]. 1995,12(6):2962
    [110]Serrano J A,Nematipour G R,Garlin D M.Dietary protein requirement of the red drum(Sciacnops ocellatus) and relative use of dietary carbohydrate and lipid[J].Aquaculture,1992,101:283-291
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