摘要
目的：以经络腧穴理论为指导,采用生物化学、行为学等方法,研究足三里穴位注射对实验性胃黏膜损伤大鼠胃功能的影响,为足三里穴位注射治疗胃黏膜损伤性疾病的临床应用提供实验依据。
方法：以健康SD大鼠为实验对象,随机分为空白对照组(简称空白组)、模型对照组(简称模型组)、足三里穴位注射硫酸罗通定组(简称穴药组)、肌肉注射硫酸罗通定组(简称肌注组)、足三里穴位注射注射用水组(简称穴水组)。空白组以0.9%生理盐水灌胃,不进行任何治疗。其余各组动物均以10%冰醋酸灌胃造成实验性胃黏膜损伤模型,造模后除模型组不进行治疗,穴药组、肌注组、穴水组分别施以罗通定穴位注射双侧“足三里”、罗通定肌肉注射以及注射用水穴位注射双侧“足三里”,观测各组动物内脏痛积分、胃排空、胃液以及胃黏膜溃疡指数。
结果：(1)治疗前基线比较,各组大鼠的体重和痛阈差异无统计学意义(P>0.05),具有可比性。
(2)内脏痛行为学检测结果：治疗后30min内,空白组的疼痛积分为0,分别与其余各组比较,差异有显著性意义(P<0.05)；模型组疼痛积分明显增多,分别与其余各组比较,差异有显著性意义(P<0.05)；穴药组的疼痛积分明显减少,分别与模型组、肌注组、穴水组比较,差异有显著性意义(P<0.05)；肌注组与穴水组比较,差异无显著性意义(P>0.05)。镇痛率的结果：治疗后30min穴药组的镇痛率高于肌注组、穴水组,差异有显著性意义(P<0.05),肌注组与穴水组相当,差异无显著性意义(P>0.05)。
(3)胃排空检测结果：治疗1次后,空白组的胃排空率明显高于其余各组,差异有显著性意义(P<0.05)；穴药组胃排空率明显低于空白组和模型组,差异有显著性意义(P<0.05)；肌注组、穴水组胃排空率与模型组比较均无显著差异(P>0.05),二者之间亦无显著差异(P>0.05)。
(4)胃液检测结果：治疗3次后,①穴药组的胃液量明显多于空白组、模型组、肌注组,差异有显著性意义(P<0.05),与穴水组无显著差异(P>0.05),其余各组之间差异无显著性意义(P>0.05)；②穴水组的胃液PH值高于空白组、模型组和肌注组,差异有显著性意义(P<0.05)；③穴药组和穴水组胃液的NaOH滴定量明显低于空白组,差异有显著性意义(P<0.05),穴药组和穴水组之间比较差异无显著性意义(P>0.05),空白组、模型组和肌注组三者之间两两比较差异无显著性意义(P>0.05)。
(5)胃黏膜溃疡指数检测结果：治疗3次后,空白组的溃疡指数为0,分别与其余各组比较,差异有显著性意义(P<0.05)；模型组的溃疡指数最高,分别与其余各组比较,差异有显著性意义(P<0.05)；穴药组的溃疡指数明显减少,分别与模型组、肌注组、穴水组比较,差异有显著性意义(P<0.05)；肌注组和穴水组比较,差异无显著性意义(P>0.05)。
结论：足三里穴位注射罗通定可明显减少实验性胃黏膜损伤大鼠内脏痛疼痛积分,抑制胃黏膜损伤引起的胃排空,促进胃液排出,抑制胃酸分泌,降低溃疡指数,在减轻疼痛、保护胃黏膜、改善胃功能方面作用显著,具有药效与穴效的协同作用。
Purpose:To take TCM meridians and acupoint Theory as the guide, using the means of biochemistry and ethology, we studied the effect of the point of "Zusanli (ST36)"injection for the experimental gastric mucosal lesions, for the theory of the point of Zusanli injection for gastric mucosal lesions in clinic.
Method:We divided randomly the healthy SD rats into blank comparison group (short for the blank group),model comparison group (short for the model group), Zusanli acupuncture point injection with Rotundine group (short for point medicine group),muscle injection with Rotundine group (short for muscle injection group), Zusanli acupuncture point injection with water for injection group (short for the water group).In addition to the blank group,the remaining groups were made by glacial acetic acid to animal models having gastric mucosal lesions. Respectively groups were injected Zusanli with the medicine of Rotundine,injected muscle with Rotundine and injected Zusanli with water for injection, then observed encelialgia ethology, gastric emptying, ulcer index,gastric acid and gastric fluid.
Results:(1)the pre-treatment baseline comparison results:the group of weight and pain threshold dependence were compared with no statistical significance of differences (P>0.05)and were comparabilitable.(2) encelialgia ethology observations:after treatment 30min, the blank group's pain integral was zero,with the remaining groups of comparison, there were significant difference (P＜0.05).Point medicine group's pain integral reduces the number, compared with the model group, muscle injection group and the water group,had significant difference (P＜0.05).The model group's pain integral marked increased in comparison with the remaining groups, a significant difference (P＜0.05).Muscle injection group with the water group, had no significant difference (P>0.05).Analgesic rate:After treatment 30min, point medicine group compared with muscle injection group and the water group had a significant difference (P＜0.05).Muscle injection group had no significant difference (P>0.05).(3)gastric emptying rate detection:the gastric emptying rate of the blank group was higher than the others and had significant difference (P<0.05).the point medicine groups were lower than the remaining groups,having significant difference (P＜0.05)compared with the blank group and the model group.Muscle injection group and the water group were lower than the model group, but having no significant difference (P>0.05)between each other. (5)ulcer index detection:after three days treatment, the blank group's ulcer index was zero and had significance (P<0.05) to the other four. The Point medicine groups decreasing obviously had significant difference (P＜0.05)compared with the model groups,the muscle injections and the water groups. The model group's was the severest of all groups.Muscle injection groups had no difference (P>0.05)with the water groups.(6) gastric acid and gastric fluid. Detection:①the PH value of the water group had significance (P＜0.05)to the blank group,the model group and muscle injection groups.②the gastric fluid quantity of point medicine group was more than the others and had significant difference (P＜0.05)to the blank groups, the model groups and muscle injection groups.③NaOH titer of point medicine group and the water group was fewer than the blank groups,the model groups and muscle injection groups, and had significant difference (P＜0.05) compared with the blank groups.The point medicine groups had no significance (P>0.05) with the water groups.It had no difference (P>0.05)among the blank groups, the model groups and the muscle injection groups.
Conclusion:Comparing with muscle injection with Rotundine, Zusanli acupuncture point injection with water for injection,Zusanli acupuncture point injection with Rotundine could obviously low down experimental gastric mucosal lesions rats'pain integral, inhibites gastric emptying, degrades ulcer index, promotes gastric fluid externalization and inhibites gastric acid secretion.
引文
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