摘要
目的：通过检测口腔粘膜下纤维性变(oral submucos fibrosis, OSF)组织中TLR2的表达情况,初步探讨TLR2在OSF发病中的作用。
方法：收集颊部OSF组织30例(早、中、晚期各10例)及正常的口腔颊粘膜组织5例,采用免疫组化SABC法检测各组织中的TLR2的表达情况。观察阳性反应程度,计数每高倍视野TLR2阳性细胞绝对数并计算阳性细胞所占百分率
结果：(1)在正常口腔颊粘膜组织中TLR2呈阳性表达,而在OSF组织中TLR2表达明显增强(P<0.05),且主要表达于粘膜下组织。
(2)OSF各期组织比较,粘膜下层TLR2阳性细胞百分率无显著性差异(P>0.05)；而OSF晚期粘膜下层组织中,每高倍视野TLR2阳性细胞绝对数比OSF早、中期明显减少,且差异有统计学意义(P<0.05)。OSF早、中期比较,每高倍野TLR2阳性细胞绝对数无显著性差异(P>0.05)。
结论：与正常口腔粘膜相比,OSF组织中TLR2的表达增高,提示TLR2可能在OSF发病中起一定的促进作用。
Objective:To investigate the expression and distribution of Toll-like receptor 2 (TLR2) in oral submucous fibrosis (OSF), and further discuss its possible role in pathology of OSF.
Methods:The SABC immunohistochemistry was used for detecting the expression and distribution of TLR2 in tissues of 5 normal controllers and 30 patients suffered from OSF(10 cases of early,intermediate and advanced stage, respectively).The positive reaction was observed and the absolute number and the percentage of positive cells were counted.
Results:(1) A positive expression of TLR2 was detected in normal oral mucosa, and a significant increasing of expression could be found in OSF(P<0.05).The expression of TLR2 in OSF was mainly detected in submucosal connective tissues.
(2) There was no significant difference between each stage of OSF in the percentage of TLR2 positive cells in the submucosa(P>0.05).When the absolute number of TLR2 positive cells in the submucosa at each stage of OSF was compared, it was decreased significantly at advanced stage, in comparison to early and intermediate stage(P<0.05), and no significant difference between early and intermediate stage (P>0.05).
Conclusions:Comparing with the normal oral mucosa, the expression of TLR2 was significantly increased in OSF tissues.It indicated that TLR2 may contribute to the pathogenisis of OSF.
引文
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