摘要
本论文对利用一种真菌（DT菌）处理农作物秸秆以提高
其营养价值进行了研究。农作物秸秆的中性洗涤纤维（NDF）
在矿物质液体培养基中经DT菌发酵30d后，损失率高于未经
DT菌发酵的对照组中NDF损失率（P＜0.01）；同时试验组发
酵溶液中葡萄糖含量高于对照组溶液中葡萄糖的含量
（P＜0.01）。分析了在25℃下DT菌对农作物秸秆进行10d、
20d、30d固体发酵后农作物秸秆各种参数。不同发酵时间的
农作物秸秆中NDF、酸性洗涤纤维（ADF）、纤维素和半纤维
素含量比对照组秸秆中的含量明显减少（P＜0.05）；在发酵10d
后，发酵秸秆的酸性洗涤木质素（ADL）含量和体外干物质消
化率与对照组相比没有显著变化（P＞0.05），随着发酵时间的
延长，其ADL含量明显低于对照组（P＜0.05），其体外干物质
消化率高于对照组（P＜0.05）。消化试验中试验组发酵后玉米
秸的干物质（DM）、NDF、ADF、纤维素和半纤维素表观消化率
显著高于对照组（P＜0.05）。
Improving the nutrition of crop straw (Maize straw and Wheat
straw) treated with fungi ?DT fungus was studied. After a 30-day
of liquid fermentation, The loss rate of neutral detergent fiber (NDF)
of crop straw, which was treated with fungi in mineral culture
medium, was more than that of the control (P<0.0 1). At the same
time, the content of glucose in the fermentation solution was more
than that of the control (P<0.0 1). The fungal fermented crop straw
was analyzed for different parameters after a fermentation period of
10, 20 and 30 days at 25 0C. The content of NDF, acid detergent
fiber (ADF), cellulose and hemicellulose of the fermented straw
after different time were less than those of the control (P<0.05)
Content of acid detergent lignin and in vitro dry matter digestibility
of the straw after a 10-day fermentation were similar with those of
the control (P>0.05), and after a 20-day and a 30-day fermentation,
content of ADL of the fermented straw was less than that of the
control (P<0.05), in intro dry matter digestibility of the fermented
straw was more than that of the control (P<0.05). In digestibility
test, the apparent digestibility of dry matter (DM), NDF, ADF,
cellulose and hemicellulose of Maize straw were differently more
than those of the control (P <0.05).
引文
1 北京农业大学 主编．1979．家畜饲养实验指导．北京：农业出版社，P52-59
2 边四辈，卢旺，王加启．1999，提高秸秆营养价值的研究进展，国外畜牧科技，26(5)：13-18
3 蔡得华，胡松庭，丁立孝等，1999．高等真菌优化麦秸与氨化麦秸做饲料育肥黄牛的对比试验．饲料研究，(12)：2-5
4 陈敏华．1987．多纤维饲料资源的开发及利用，饲料研究，(7)：20-24
5 东北农学院 主编．1979．家畜饲养学．北京：农业出版社
6 《非常规饲料资源的开发与利用》研究组 编著．1996．北京：中国农业出版社，P18
7 顾岱芳，卞殿明．1999．饲用酶制剂作用及应用．饲料研究，(2)：23-25
8 贵州农学院．1993．生物统计附试验设计．北京：农业出版社．
9 韩建林，王锋，张丽芹．1996．利用白腐真菌提高秸秆利用率的研究进展．饲料博览，9(1)：19-20
10 韩建林，徐振清，李精鸿等．1996．白腐真菌可提高秸秆利用率．中国饲料，(24)：28-29
11 韩友文主编．1996，饲料与饲养．黑龙江：东北农业大学
12 何明清 主编．1994．动物微生态学．北京：中国农业出版社，P107-102
13 贺建华，陈立祥．1991．低质牧草和粗饲料的改良．国外畜牧学-饲料，(1)：10-17
14 华中农业大学，南京农业大学 主编．1998．微生物学，北京：农业出版社，P116-121
15 黄隆广，汤维清，徐志辉．1989．微生物发酵稻草营养价值研究．饲料工业，(7)：14-15
16 李慧蓉．1996．白腐真菌在碳素循环中的地位和作用．微生物通报，23(2)：105-109
17 刘国华，蔡辉益．2000，动物微生态理论在饲料工业中的应用．饲料工业，21(3)：1-4
18 刘建新，杨振海，叶均安等．1999．青贮饲料的合理调制与质量评定标准．饲料工业，20(3)：4-7
19 刘晓牧，李福昌，王中华等，2000．白腐真菌与秸秆饲料的有效利用．饲料研究，(1)：42-43
20 卢德勋．1993．内蒙古畜牧科学，特刊
21 卢庆萍，王加启．1999．秸秆处理技术的研究，中国饲料，(10)：26-29．
22 陆治年，李文立．1990．秸秆加工研究-化学处理法概况，中国奶牛，(3)：25-27
23 孟庆翔，熊易强．1991．辐射处理提高秸秆营养价值，饲料工业，12(1)：13-14
24 聂柱山，玉兰．1992．利用生物技术提高纤维性饲料饲用价值，饲料研究，(1)：15-16
25 邱建德 主编．1998．国际通用统计软件SAS与多元统计分析．内部资料
26 瞿明仁，晏向华，黎观红等．1999．饲用真菌纤维素酶研究与应用．饲料工业，20(12)：30-32
27 王凤国．1994，粗饲料加工技术研究动向及进展，中国奶牛，(5)：25-27
28 王建华，白韵如．1997．开发秸秆发酵饲料的思考．中国饲料，(14)：13-16
29 王宜磊，孙迅，邓振旭．1998．木素生物降解研究进展，微生物学杂志，18(1)：48-51
30 许振英．1984．多纤维饲料在畜牧业的应用．饲料研究，(5)：8-13
31 杨凤 主编．1993．动物营养学．北京：农业出版社，P114-117
32 杨胜 主编．1993．饲料分析及饲料检测技术．北京：北京农业大学出版社
33 杨新美 主编．1996．食用菌栽培学．北京：中国农业出版社，P86-90
34 张玉明，陆恒．1999．秸秆与青绿饲料的营养及其科学利用(上)，农村养殖技术，(9)：27
35 中国农机学会农机化学会科技交流中心编．1996．农作物秸秆利用技术与设备．北京：中国农业出版社，P1-6
36 中国土壤学会农业专业委员会 编．1984．土壤农业化学常规分析方法．北京：科学出版社，P300-312
37 张勇，朱宇旌，周安国．1999．微生态营养及其研究概况，饲料研究，(9)：17-19
38 庄乾明．1999．秸秆青贮、氨化、微生物发酵3种方法的比较及其相关性的研究．草食家畜，(1)：40-41
39 扇元敬司．1994．微生物学．日本东京：讲谈社．P198
40 Agosin E., Monties B. and Odier E. 1985. Structural changes in wheat straw components during decay by lignin-degrading white-rot fungi in relation to improvement of digestibility for ruminants. J. Sci. Food Agric. 36:925-935
41 Agosin E., Toller M.T., Brillonet J.M. et al.1986. Fungal pretreatment of wheat straw: effects on the biodegradability of cell walls, structural polysaccharides, lignin and phenolic acids by rumen microorganisms. J. Sci. Food Agric. 37:97-106
42 Akin D.E., Setharaman A., Morrison Ⅲ W.H. et al.1993. Microbial delignification with white rot fungi improves forage digestibility. Applied and Environment Microbiology, 59 (12):4274-4282
43 Akin D.E., Rigsby L.L., Setharaman A. et al.1995. Alterations in structure, chemistry, and biodegradability of grass lignincellulose treated with the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus. Applied and Environment Microbiology, 61(4):1591-1598
44 Akin D.E., Morrison Ⅲ W.H., Rigsby L.L. et al.1996. Biological delignification of plant components by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus. Anim. Feed Sci. Technol. 63: 305-321
45 Akira A. 1988. Feed analyses based on the carbohydrates and its application to the nutritine value of feeds. In: Memoirs of National Institute of Animal Industry, No 2．日本前田印刷株式会社, 34-40
46 Almendros G., Mart-nez A.T., Gonz lez A.E. et al. 1992. CPMAS ~(13)C NMR study of lignin preparations from wheat straw transformed by five lignocellulose-degrading fungi. J. Agric. Food Chem 40: 1297-1302
47 Buxton R.D. and Redfearn D.D. 1997. Plant limitions to fiber digestion and utilization. J.Nutr. 127:814-818
48 Chaudhry A.S. 1998. Chemical and biological procedures to upgrade cereal straws for ruminants. Nutrition Abstracts and Reviews (Series B), 68(5): 319-331
49 Chesson A. 1988. Lignin-polysaccharide complexes of the plant cell wall and their effect on microbial degradation in the rumen, Anim. Feed Sci. and Technol. 21: 219-228
50 Cornu A., Besle J.M. et al.1994. Lignin-carbohydrate complexes in forages: structure and consequences in the ruminal degradation of cell-wall carbohydrates. Reprod. Nutr. Dev. 34:385-398
51 Cowling E.B. 1975. Physical and chemical constrains in the hydrolysis of cellulose and lignocellulosic materials. In: Wick C.R. (ed), Cellulose as a chemical and energy resource. 163
52 Crawford R.L. and Crawford D.L. 1984. Recent advances in Studies of the mechanisms of microbial degradation of lignin. Enzyme and Microbial Technology, 6:433-442
53 Dahiya D. S., Mann N. S., Khatta V. K. et al.1999. Effect of fungal treatment on the quality of cellulosiccrop residues. Indian Dairy Science, 52(3): 183-187
54 Eggert C., Temp U. and Eriksson K.E.L. 1995. The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus:purification and characterization of the laccase. Applied and Environmental Microbiology, 62(4):1151-1158
55 FAO.1994. Total population, agriculture population. Production Yearbook 38, 63-77
56 Gupta V.K. and Langar P.N. 1988. Pleurotus florida for upgrading the nutritive value of wheat straw. Biological Wastes. 23:57-64
57 Hartley R. D.,Jones E. C., King N.J. et al. 1974. Modified wood waste and straw as a potential components of animal feeds. J. of the Sci. Of Food and Agric. 25:433-437
58 Higuchi T. 1982. Biodegradation of lignin: biochemistry and potential applications. Experientia, 38:159-166
59 Homb T.1984. Wet treatment with sodium hydroxide. In: Straw and other fibrous by-products as feeds, Sundstol F., Owen E. (eds.), Amsterdam, Netherlands; Elsevier Science Publishers BV.
60 Jackson M. G.1977. Review article: the alkali treatment of straw. Anim. Feed Sci. And Technol. 2:105-130
61 Jalc D., Zitnan R. and Nerud F. 1994. Effect of fungus-treated straw on ruminal fermentation in vitro. Anim. Feed Sci. and Technol. 46:131-141
62 Jalc D., Nerud F. et al. 1996. Effect of white rot basidiomycetes-treated wheat straw on rumen fermentation in artificial rumen. Reprod. Nutr. Dev.36:263-270
63 Jung H.G., Valdez F.R., Abad A.R. et al.1992. Effect of white rot basidiomycetes on chemical composition and in vitro digestibility of oat straw and alfalfa stems. J. Anim. Sci. 70:1928-1935
64 June H.G. and Allen M.S. 1995. Characteristics of plant cell walls affecting intake and digestibility of forages by ruminants. J. Anim. Sci. 73:2774-2790
65 Kahlon S.S., and Dass S.K. 1988. Biological conversion of paddy straw into feeds. Biological Wastes, 22:11-21
66 Kaplan D.L. and Roy H. 1980. Decomposition of lignin by microorganisms. Soil Biol. Biochem. 12:65-75
67 Karunanadaa K., Fales S.L., Varga G.A. et al. 1992. Chemical composition and biodegradability of crop residues colonized by white rot fungi. J. Sci. Food Agric, 60:105-112
68 Karunanadaa K. and Varga G. A. 1996. Colonization of crop residues by white-rot fungi: cell wall monosaccharides, Phenolic acids, ruminal fermentation characteristics and digestibility of cell wall fiber components in vitro. Anim. Feed Sci. Technol. 63:273-288
69 Kirk T.K., Higuchi T. and Chang H.M. 1980. Lignin biodegradation: microbiology, chemistry and potential applications. Boco Raton, Florida, USA; CRC Press Inc.
70 Macki R.I., Kistener A. 1985. Some frontiers of research in basic ruminant nutrition. South African Journal of Animal Science 15:72-85
71 MAFF. 1986. Feed straw to housed ewes. Minstry of Agriculture, Fisheries and Food, UK; Sheet No. 3016
72 Morris J.G. 1976. Oxygen and its obligate anaerobes. Journal of Applied Bacteriology, 40:229
73 Ogawa K. 1987. Microbiological utilization of rice straw. 宫崎大学农学部研究报告, 34(2): 281-288
74 Owen E., Klopfenstein T., Urio N.A. 1984. Treatment with other chemicals. In: Straw and other fibrous by-products as feed. Sundstol F., Owen E.(eds.), Amsterdam, Netherlands; Elsevier Science Publishers BV.
75 Pal D., Kamra D.N. and Pathak. 1999. Changes in chemical composition and in sacco degradability of wheat straw treated with Pleurotus sajorcaju and P. Ostreatus. Inter. J. Anim. Sci. 14:61-67
76 Rajarathnam S., Wankhede D.B., Bano Z. 1987. Degradation of rice straw by Pleuratus flabellatus. J. Chem. Technol. Biotechnol. 37:203-214
77 Rangaswami G., Kandaswami T.K. and Ramaswami K. 1975. Pleurotus sajor-caju (Fr.) Singer: a protein rich nitrogen fixing mushroom fungus. Curt. Sci. 44: 403-405
78 Reade A.E., McQueen R.E. 1983. Investigation of white-rot fungi for the conversion of poplar into a potential feedstuff for ruminants. Can. J. Microbiol. 29:457-463
79 Reid I.D. and Seifert K.A. 1982. Effect of an atmosphere of oxygen on growth, respiration, and lignin degradation by white-rot fungi. Can. J. Bot. 60:252-260
80 Rexen F.P., Stigsen P., Kristensen V.F, 1975. The effect of a new alkali technique on the nutritive value of straws. In: Proceeding of the 9th Congress of Feed Manufactures, University of Nottingham, P65-82
81 Staniforth A.R. 1982. Straw for fuel, feed and fertilizer. Ipswich UK; Farming Press Ltd.
82 Sundstol F. 1988. 2. Feedstuff. 5. Straw and other fibrous by-products. Livestock Production Science, 19:137-158
83 Suzuki Y., Okanok K., Nakajima T. et al.1994. Whiterot fungal treatment of unsterilized wheat straw by Pleurotus salmoneostramineus to improve its digestibility. Anim. Sci. Technol. (Jpn).66(5): 406-411
84 Ulmer D.C., Leisola M.S.A., Schmidt B.H. et al.1983. Rapid degradation of isolated lignins by Phanerochaete chrysosporium. Applied and Environmental Microbiology,45(6): 1795-1801
85 Varga G.A. and Kolver E.S. 1997. Microbial and animal limitations to fiber digestion and utilization.J. Nutr. 127:918-823
86 Walker H.G. 1984. Physical treatments. In: Straw and other fibrous by-products as feed, Sundstol F, Owen E. (eds.), Amsterdam,Netherlands; Elsevier Science Publishers BV.
87 Wicklow D.T. and Detroyand A.S. 1980. Differential modification by fungi colonists of ruminants dung:ecology implication. Mycologia, 72:1065-1076
88 Wicklow D.T., Langie R., Crabtree S. et al.1984.Degradation of lignocellulose in wheat straw versus hardwood by Cythathus and related species(Nidulariacene). Can. J. Microbial, 30:633-636
89 Wilson P.N. 1974. Possibilities of processing straw for animal feed. A report on straw utilization conference,Oxford,5 December, Agricultural Development and Advisory Service, P19-24
90 Wilson R.K., Pigden W.J. 1964. Effect of sodium hydroxide treatment on the utilization of wheat straw and poplar wood by rumen microorganism. Can. J. of Anim. Sci. 44:122-123
91 Zadrazil F.1984. Microbial conversion of lignocellulose into feed. In: Straw and other fibrous by-products as feed, Sundstol F., Owen E.(eds.),Amsterdam, Netherlands; Elsevier Science Publishers BV.
92 Zadrazil F. 1987. White-rot fungi and mushrooms grown on cereal straw: aim of the process, final-products and scope for the future.In:Degradationof lignocelluloses in ruminants and in industrial processes, Barking, UK;Elsevier Applied Scientific Publishers.
