摘要
目的：研究人类直肠组织中是否存在多环芳烃(PAHs);分析PAHs在直肠癌组织与非直肠癌组织中含量的差异；探讨多环芳烃在人类直肠癌发生过程中的作用。
方法：直肠癌组织、癌旁组织和非直肠癌患者的直肠组织中的PAHs分别经超声提取,固相萃取净化和高效液相色谱荧光分析。
结果：三组标本中共检出4种PAHs,分别是菲、芘、2-甲基蒽和苯并(a)芘。其中苯并(a)芘的浓度最高,且在直肠癌组织中高达(1129.3±333.9)ng/g；芘、2-甲基蒽和苯并(a)芘在直肠癌组织和癌旁组织中的含量均高于非直肠癌患者的直肠组织,差异有统计学意义(P<0.05)；菲在三组标本中的含量差异无统计学意义(P>0.05)；4种PAHs在直肠癌组织与癌旁组织中的含量差异均无统计学意义(P>0.05)。
结论：(1)首次发现人类直肠组织中存在PAHs;
(2)人类直肠组织对PAHs有很强的生物富集能力；
(3)直肠癌组织中PAHs的含量高于非直肠癌组织,差异有统计学意义(P<0.05)：
(4)人类直肠组织中PAHs的含量与直肠癌的发生有一定的相关性。
Objective:To investigate if there were polycyclic aromatic hydrocarbons (PAHs) in human rectum; To evaluate the difference of the contents of the PAHs between rectal tumor and normal rectum; And to investigate the effect of PAHs in the occurrence of human rectal tumor.
Methods:PAHs in human rectal tumor, adjacent tissues and normal rectum were extracted by ultrasonic wave. And then the extracts were cleaned up by solid phase extraction and analyzed by high performance liquid chromatography with fluorescence detector.
Results:Four kinds of PAHs were detected, which were phenanthrene, pyrene, 2-methylanthracene and benzo (a) pyrene. The content of benzo (a) pyrene in rectal tumor was more than other PAHs, which was (1129.3±333.9) ng/g; The contents of pyrene, 2-methylanthracene and benzo (a) pyrene in both rectal tumor and adjacent tissues were higher than normal rectum, the differences were statistically significant (P<0.05). The contents of phenanthrene in the three kinds of tissues were not statistically significant (P>0.05). The contents of the four PAHs were not statistically significant between rectal tumor and adjacent tissues (P>0.05).
Conclusion:(1) It is the first reported that PAHs were found in human rectal tissue. (2) Human rectal tissue has extremely strong ability of bioconcentrating PAHs. (3) The contents of PAHs in rectal tumor are higher than normal rectum, the differences were statistically significant (P<0.05). (4) The contents of PAHs and the occurrence of human rectal tumor may have a certain correlation.
引文
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