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- 英文篇名:Studies on the Physiological Efficacy and Safety of Chinese Herbal Medicine on Litopenaeus vannamei
- 作者:黄辉 ; 潘鲁青 ; 宋梦思
- 英文作者:HUANG Hui;PAN Luqing;SONG Mengsi;The Key Laboratory of Mariculture (Ocean University of China), Ministry of Education;
- 关键词:中草药 ; 凡纳滨对虾 ; 生理功效 ; 安全评价
- 英文关键词:herbal formula;;Litopenaeus vannamei;;physiological effect;;safety evaluation
- 中文刊名:海洋湖沼通报
- 英文刊名:Transactions of Oceanology and Limnology
- 机构:中国海洋大学海水养殖教育部重点实验室;
- 出版日期:2019-02-15
- 出版单位:海洋湖沼通报
- 年:2019
- 期:01
- 基金:国家海洋公益性行业科研专项课题(201305005)资助
- 语种:中文;
- 页:148-155
- 页数:8
- CN:37-1141/P
- ISSN:1003-6482
- 分类号:S948
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摘要
本文研究了中草药对凡纳滨对虾的生理功效和安全性评价。实验将基础饲料作为对照组饲料,在基础饲料中分别添加0.1%黄芪多糖、0.1%黄芪多糖+0.05%大蒜素、0.1%绿原酸、0.1%绿原酸+0.05%大蒜素作为四种实验组饲料(G1-G4),养殖凡纳滨对虾21d,再进行7d攻毒实验。结果表明:3种中草药配方对凡纳滨对虾血细胞数量、吞噬率、血浆抗菌活力和总抗氧化能力影响显著(P<0.05),而对照组无明显变化。G1、G2处理组对虾血细胞数量6d后显著高于对照组,G3、G4处理组与对照组无显著差异,各处理组血细胞吞噬率、血浆抗菌活力分别于10d、3d后显著高于对照组水平,除G1处理组外,各处理组血浆总抗氧化能力10d后显著高于对照组,之后各指标均保持稳定;攻毒7d后,各处理组对虾累积死亡率显著低于对照组,累积死亡率为G4 0.05),G1、G2和G4处理组肝胰腺泡状细胞明显增多,其余与对照组组织结构类似,形态正常。由此可见,3种中草药配方对凡纳滨对虾免疫防御和抗氧化具有明显的增强作用,提高了对虾抵御病原菌能力,并对肝胰腺无毒副作用,以G4(0.1%绿原酸+0.05%大蒜素)配方效果最佳。 In this study, a feeding trial followed by a challenge test were performed to evaluate the effects of four herbal formulae(different combinations of Astragalus polysaccharides(APS), chlorogenic acid(CGA) and allicin) on the non-specific immune response and metabolic damage of Litopenaeus vannamei. Shrimp were fed with five diets, one as the control(basal diet with no herbal formulae) and four herbal formula diets(G1-G4, basal diet supplemented with 0.1% APS, 0.1% APS + 0.05% allicin, 0.1% CGA, 0.1% CGA + 0. 05% allicin, respectively) for 21 days. After that, shrimp were challenged with Vibrio harveyi with the cumulative mortality of shrimp recorded for 7 days post challenge. The results showed that herbal formulae had significant effects on the non-specific immune indicators and disease resistance against V. harveyi. The total hemocyte count increased significantly in G1 and G2 after 6 days feeding, then tended to be stable after 10 days; the phagocytic capacity and antibacterial activity of G1 through G4 were significantly higher(P<0.05) than those of control after 10 days and 3 days feeding, respectively, then these two immune indicators were stable. The plasma total antioxidant capacity was significantly enhanced in G2 through G4 but not in G1. In addition, after 7 days challenge test, the cumulative mortality of shrimp each treatment group was significantly lower than that of control, and order of the cumulative mortality rate was G4
引文
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