绵羊脂联素基因(ADIPOQ)多态性及其与生长及胴体性状关联性分析

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英文篇名：Polymorphisms of ADIPOQ Gene and Their Association with Growth and Carcass Traits in Sheep 作者：安清明 ; 周辉通 ; 吴震洋 ; 罗玉柱 ; Jon ; G.Hickford 英文作者：AN QingMing;ZHOU HuiTong;WU ZhenYang;LUO YuZhu;Jon G.Hickford;Tongren University;Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University;Gene-Marker Laboratory, Lincoln University; 关键词：绵羊 ; ADIPOQ基因 ; 性别差异 ; 生长性状 ; 胴体性状 英文关键词：sheep;;ADIPOQ gene;;gender-specific;;growth traits;;carcass traits 中文刊名：中国农业科学 英文刊名：Scientia Agricultura Sinica 机构：铜仁学院;甘肃农业大学草食动物生物技术重点实验室;新西兰林肯大学基因标记实验室; 出版日期：2019-05-16 出版单位：中国农业科学 年：2019 期：10 基金：贵州省科技计划项目(黔科合基础[2018]1161);; 贵州省普通高等学校科技拔尖人才支持计划(黔教合KY字[2017]089);; 铜仁市科技计划项目(铜市科研[2016]18号-5);; 铜仁学院博士科研启动项目(trxyDH1624) 语种：中文; 页：146-156 页数：11 CN：11-1328/S ISSN：0578-1752 分类号：S826

摘要

【目的】检测绵羊ADIPOQ基因多态性及连锁现状,评估该基因突变对绵羊生长及胴体性状的影响,以期丰富绵羊相关重要经济性状的分子遗传研究基础。【方法】以8个不同品种的商品绵羊为研究对象,利用PCR-SSCP方法检测ADIPOQ基因Exon-1区和Exon-2区变异,利用GLMs模型进行评估该基因突变对绵羊生长及胴体性状的影响。【结果】绵羊ADIPOQ基因Exon-1区和Exon-2区共检测到13个突变位点,其中Exon-2区发现的c.46T/C突变导致编码氨基酸p.Tyr16His转变。Exon-1区等位基因A1和B1为优势等位基因,Exon-2区等位基因A2和D2为优势等位基因,且两个区域的等位基因均存在种群差异,大多数品种在两个区域中的变异为中度多态(0.250.5),特克塞尔、派伦代和丘陵陶塞特羊在Exon-2区为低度多态(PIC<0.25);两段区域间存在的突变位点为高度连锁,且趋向于共同遗传(D’=0.952,r2=0.365)。关联分析结果表明,ADIPOQ基因Exon-1区变异对绵羊生长性状存在性别差异,携带等位基因A1的公羔具有较低的断尾重、断奶重和断奶前生长速度(P<0.05),而携带等位基因A1的母羔却与生长性状无显著关联;携带等位基因B1的公羔与生长性状无显著关联,但携带等位基因B1的母羔却具有较高的断尾重(P<0.05);同时发现基因型为B1B1的公羔个体具有更高的断尾重和断奶重(P<0.05);胴体性状关联分析结果表明,携带等位基因A1的群体具有较低的热胴体重、腰部瘦肉量、后腿瘦肉量和总瘦肉量(P<0.05),携带等位基因B1的群体则具有较高的后腿瘦肉量、后腿瘦肉比例和较低的肩部瘦肉比例(P<0.05);基因型为B1B1的个体均有较高的热胴体重、腰部瘦肉量、后腿瘦肉量和总瘦肉量(P<0.05)。【结论】绵羊ADIPOQ基因的两段区域具有丰富的多态性,Exon-2区域中的c.46T/C为非同义突变。Exon-1区变异影响绵羊的生长性状和胴体性状,淘汰携带等位基因A1的个体和选留存在等位基因B1的个体、或留存B1B1基因型的个体和淘汰A1A1的个体,均可有效改善绵羊后代群体的部分生长性状和胴体性状。
        【Objective】In this study, polymorphisms and linkage relationship of ovine ADIPOQ(adiponectin) gene were investigated and their effects on some growth and carcass traits were estimated, so as to enrich the molecular genetic data for sheep.【Method】Mutations in Exon-1 and Exon-2 of ADIPOQ gene were detected by PCR-SSCP in 8 commodity sheep populations, and the relationship between mutations in growth and carcass traits in New Zealand(NZ) Romney lambs was investigated using General Linear Models(GLMs). 【Result】In total, thirteen SNPs were detected in Exon-1 and Exon-2 regions of ovine ADIPOQ gene, and the nucleotide substitution c.46 T/C in Exon-2 resulted in amino acid change(p.Tyr16 His). Allele A1 and B1 were the dominant allele in Exon-1, Allele A2 and D2 were the dominant allele in Exon-2, and there were difference of allele frequencies between these two regions. The majority population was moderately polymorphic in all regions(PIC<0.5), except Texel, Perendale and Dorset Down was low polymorphic in Exon-2 region(PIC<0.25), and there was a high linkage relationship of these mutation and tend to shared genetic linkages(D'=0.952, r2=0.365). The association analysis showed that the mutations in Exon-1 region of ovine ADIPOQ gene had different effects on growth traits of male and female lambs. In male lambs, individuals with possessing allele A1 had lower tailing weight, weaning weight and pre-weaning growth rate than those no-possessing(P<0.05), but no associations were detected in female lambs(P>0.05). In female lambs, individuals with possessing allele B1 had higher tailing weight than those no-possessing(P<0.05), however, no associations were detected in male lambs(P>0.05). And individuals with possessing B1 B1 had higher tailing weight and weaning weight in male lambs. The carcass traits association analysis results showed that individuals with possessing allele A1 had lower hot-carcass weight, loin yield, leg yield and total yield than those no-possessing(P<0.05), and individuals with possessing allele B1 had higher leg yield, proportion of led yield and lower proportion shoulder yield than those no-possessing(P<0.05), individuals with possessing B1 B1 had higher hot carcass weight, loin yield, leg yield and total yield(P<0.05). 【Conclusion】Exon-1 and Exon-2 of ovine ADIPOQ gene had abundant polymorphisms, and SNP c.46 T/C was non-synonymous. The mutations of Exon-1 of ovine ADIPOQ gene might affect some growth traits and carcass traits, and selecting sheep with allele B1 and genotype B1 B1, or eliminating ones with allele A1 and A1 A1 could improve some growth traits and carcass traits of Romney sheep.

引文

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