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在“Elsevier电子期刊”中，命中：57条，耗时：小于0.01 秒

在所有数据库中总计命中：23条

1.An improved method for high-level soluble expression and purification of recombinant amyloid-beta peptide for in vitro studies

作者：Gaurav Chhetri^a ; ¹ ; Tripti Pandey^a ; ¹ ; Ramesh Chinta^a ; Awanish Kumar^b ; ^{drawanishkr@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Timir Tripathi^a ; ^{timir.tripathi@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{ttripathi@nehu.ac.in" class="auth_mail" title="E-mail the corresponding author}

关键词：A尾 ; amyloid-尾 peptide ; EK ; enterokinase ; GSH ; reduced glutathione ; GST ; glutathione S-transferase ; IPTG ; isopropyl 尾-d-1-thiogalactopyranoside ; Ni&ndash ; NTA ; nickel&ndash ; nitrilotriacetic acid

刊名：Protein Expression and Purification

出版年：2015

2.Expression, purification and characterization of a functional, recombinant, cold-active lipase (LipA) from psychrotrophic Yersinia enterocolitica

作者：Xiuling Ji^a ; Shan Li^a ; Baoqiang Wang^b ; Qi Zhang^a ; Lianbing Lin^a ; Zhiyang Dong^c ; Yunlin Wei^a ; ^{weiyunlin18@gmail.com" class="auth_mail" title="E-mail the corresponding author}

关键词：E. coli ; Escherichia coli ; SDS&ndash ; PAGE ; sodium dodecyl sulfate&ndash ; polyacrylamide gel electrophoresis ; p-NPB ; p-nitrophenylbutyrate ; IPTG ; isopropyl 尾-d-1-thiogalactopyranoside ; EDTA ; ethylenediaminetetraacetic acid ; BGL ; Burkholderia glumae lipase ; BCL ; B. cepacia lipase ; PAL ; Pseudomonas aeruginosa lipae ; PML ; Proteus mirabilis lipase ; YEL ; Y. enterocolitica strain KM1 lipase ; DMSO ; dimethyl sulfoxide

刊名：Protein Expression and Purification

出版年：2015

3.Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli

作者：Lesley A. Pearce^a ; ^{lesley.pearce@csiro.au" class="auth_mail" title="E-mail the corresponding author} ; Meng Yu^b ; Lynne J. Waddington^a ; Jennifer A. Barr^b ; Judith A. Scoble^a ; Gary S. Crameri^b ; William J. McKinstry^a

关键词：CedPV ; Cedar virus ; EIDs ; emerging infectious diseases ; ESI-TOF-MS ; electro-spray ionization time-of-flight mass spectrometry ; HeV ; Hendra virus ; HeV N ; Hendra virus nucleocapsid protein ; HeV NFL ; Hendra virus nucleocapsid protein (full length ; amino acid residues 1&ndash ; 532) ; HeV NCORE ; Hendra virus nucleocapsid protein CORE region (amino acid residues 1&ndash ; 402) ; IDR ; intrinsically disordered region ; IMAC ; immobilized metal affinity chromatography ; IPTG ; isopropyl-尾-d-thiogalactopyranoside

刊名：Protein Expression and Purification

出版年：2015

4.Role of substituents on the reactivity and product selectivity in reactions of naphthalene derivatives catalyzed by the orphan thermostable cytochrome P450, CYP175A1

作者：Shibdas Banerjee¹ ; Sandeep Goyal² ; Shyamalava Mazumdar ; ^{shyamal@tifr.res.in" class="auth_mail" title="E-mail the corresponding author} ;

关键词：Heme ; iron protoporphyrin IX regardless of oxidation and ligation state of the central metal ion (Fe) ; WT ; wild type CYP175A1 ; L80F ; Leucine 80 to phenylalanine mutant of CYP175A1 ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; Tm ; melting temperature ; PDB ; research collaboratory for structural bioinformatics (RCSB) protein data bank ; http ; //www.rcsb.org/pdb/ ; PMSF ; phenylmethylsulfonyl fluoride ; IPTG ; isopropyl-尾-d-1-thiogalactopyranoside ; EDTA ; ethylenediaminetetraacetic

刊名：Bioorganic Chemistry

出版年：2015

5.Identification of novel isoprene synthases through genome mining and expression in Escherichia coli

作者：Marja Ilmé ; n^a ; Merja Oja^a ; Anne Huuskonen^a ; Sangmin Lee^b ; Laura Ruohonen^a ; Simon Jung^b ; ^{jungmoongeun@sk.com" class="auth_mail" title="E-mail the corresponding author}

关键词：IspS ; isoprene synthase ; DMAPP ; dimethylallyl pyrophosphate ; MEP ; methylerythritol phosphate ; MEV ; mevalonate ; IPTG ; isopropyl 尾-D-1-thiogalactopyranoside ; CDW ; cell dry weight ; TSP ; terpene synthase ; TSP-b ; terpene synthase protein family clade b ; MSA ; multiple sequence alignment ; RNA-seq ; RNA sequencing

刊名：Metabolic Engineering

出版年：2015

6.Antigenic assessment of a recombinant human CD90 protein expressed in prokaryotic expression system

作者：Narges Yousefi-Rad^a ; Mohammad Ali Shokrgozar^b ; Mahdi Behdani^c ; Shima Moradi-Kalbolandi^b ; Mahdieh Motamedi-Rad^d ; Mahdi Habibi-Anbouhi^b ; ^{habibi_m@pasteur.ac.ir" class="auth_mail" title="E-mail the corresponding author}

关键词：CD90 ; Cluster of Differentiation 90 ; CSCs ; cancer stem cells ; exCD90 ; extra-membrane domain of human CD90 ; E. coli ; Escherichia coli ; ELISA ; enzyme-linked immunosorbent assay ; mAb ; monoclonal antibody ; pAb ; polyclonal antibody ; IPTG ; isopropyl-尾-d thiogalactopyranoside ; OD ; optical density ; DAB ; 3 ; 3&prime ; -Diaminobenzidine

刊名：Protein Expression and Purification

出版年：2015

7.Functional roles in S-adenosyl-l-methionine binding and catalysis for active site residues of the thiostrepton resistance methyltransferase

作者：Cullen L. Myers^a ; ¹ ; Emily G. Kuiper^b ; Pei C. Grant^a ; ² ; Jennifer Hernandez^b ; Graeme L. Conn^b ; John F. Honek^a ; ^{jhonek@uwaterloo.ca" class="auth_mail" title="E-mail the corresponding author}

关键词：Ala ; alanine ; Arg ; arginine ; Asn ; asparagine ; Asp ; aspartic acid ; ESI-MS ; electrospray ionization mass spectrometry ; IMAC ; immobilized metal ion affinity chromatography ; IPTG ; isopropyl 尾-D-1-thiogalactopyranoside ; ITC ; isothermal titration calorimetry ; KD ; dissociation constant ; Lys ; lysine ; nt ; nucleotide ; rRNA ; ribosomal RNA ; SAH ; S-adenosyl-l-homocysteine ; SAM ; S-adenosyl-l-methionine ; SDS&ndash ; PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis ; wt ; wild type

刊名：FEBS Letters

出版年：2015

8.Characterization of a novel y-type HMW-GS with eight cysteine residues from Triticum monococcum ssp. monococcum

作者：Zenglin Li^a ; Hongyu Li^a ; Gang Chen^a ; Chunlan Kou^a ; Shunzong Ning^a ; Zhongwei Yuan^a ; Qi Jiang^b ; Youliang Zheng^a ; Dengcai Liu^a ; Lianquan Zhang^a ; ^{zhanglianquan1977@126.com" class="auth_mail" title="E-mail the corresponding author}

关键词：aa ; amino acid(s) ; bp ; base pair(s) ; BLAST ; basic local alignment search tool ; dNTP ; deoxyribonucleoside triphosphate ; HMW-GS ; high molecular weight glutenin subunit ; IPTG ; isopropyl 尾-螖-thiogalactopyranoside ; NCBI ; National Center for Biotechnology Information ; NJ ; Neighbor-joining ; ORF ; open reading frame ; PCR ; polymerase chain reaction ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis

刊名：Gene

出版年：2015

9.Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco

作者：Long-Qun Deng^a ; Hao-Qiang Yu^a ; Yan-Ping Liu^b ; Pei-Pei Jiao^b ; Shu-Feng Zhou^a ; Su-Zhi Zhang^a ; Wan-Chen Li^a ; ^{aumdyms@sicau.edu.cn" class="auth_mail} ; Feng-Ling Fu^a ; ^{ffl@sicau.edu.cn" class="auth_mail}

关键词：ADH ; alcohol dehydrogenase ; AFP ; antifreeze proteins ; CTAB ; hexadecyltrimethylammonium bromide ; EC ; electrical conductivity ; GUS ; 尾-glucuronidase ; HSP ; heat shock protein ; IPTG ; isopropyl 尾-D-thiogalactopyranoside ; LB ; Luria-Bertani ; qRT-PCR ; quantitative real-time PCR ; REC ; relative electrical conductivity ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis

刊名：Gene

出版年：10 April, 2014

10.Generating novel recombinant prokaryotic lectins with altered carbohydrate binding properties through mutagenesis of the PA-IL protein from Pseudomonas aeruginosa

作者：Damien Keogh^a ; ¹ ; Roisin Thompson^a ; ^b ; ¹ ; Ruth Larragy^a ; ^b ; Kenneth McMahon^a ; Michael O'Connell^a ; Brendan O'Connor^a ; ^b ; Paul Clarke^a ; ^b ; ^{Paul.Clarke3@gmail.com" class="auth_mail}

关键词：PA-IL ; Pseudomonas aeruginosa lectin 1 ; PA-ILU ; untagged native PA-IL ; rPA-IL ; recombinant PA-IL ; 6HIS ; affinity purification tag comprised of 6 tandem histidine residues ; ELLA ; enzyme linked lectin assay ; iGb3 ; isoglobotriaosylceramide (Gal伪1&ndash ; 3Gal尾1&ndash ; 4Glc) ; PBS ; Phosphate Buffered Saline ; TBS ; Tris Buffered Saline ; TBST ; Tris Buffered Saline with Tween 20 ; IPTG ; Isopropyl-尾-D-thiogalactopyranoside

刊名：Biochimica et Biophysica Acta (BBA)/General Subjects

出版年：June 2014

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