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在“
Elsevier电子期刊
”中,
命中:
2,497
条,耗时:0.022988 秒
在所有数据库中总计命中:
9,846
条
1.
Going native: Complete removal of protein purification
affinity
tag
s by simple modification of existing
tag
s and proteases
作者:
Hui Chin Goh
a
;
Radoslaw M. Sobota
b
;
Farid J. Ghadessy
a
;
fghadessy@p53Lab.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author
;
Saurabh Nirantar
a
;
saurabh.nirantar79@gmail.com" class="auth_mail" title="E-mail the corresponding author
关键词:
Protein purification
;
Affinity
tag
s
;
Protease
;
Enforced colocalisation
;
Native protein
刊名:Protein Expression and Purification
出版年:2017
2.
Integration of cell harvest with
affinity
-enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual
tag
ligand
作者:
Isabel Campos-Pinto
a
;
Edith Espitia-Saloma
b
;
Sara A.S.L. Rosa
a
;
Marco Rito-Palomares
b
;
Oscar Aguilar
b
;
Miguel Aré
;
valo-Rodrí
;
guez
c
;
M. Raquel Aires-Barros
a
;
Ana M. Azevedo
a
;
a.azevedo@tecnico.ulisboa.pt" class="auth_mail" title="E-mail the corresponding author
关键词:
Aqueous two-phase system
;
Cell harvesting
;
Affinity
partitioning
;
Monoclonal antibodies
;
Downstream processing
刊名:Separation and Purification Technology
出版年:2017
3.
Novel interacting proteins identified by tandem
affinity
purification coupled to nano LC-MS/MS interact with ribosomal S6 protein kinase 4 (RSK4) and its variant protein (RSK4m)
作者:
Riqiang Liu
a
;
1
;
liuriqiang@whu.edu.cn
;
Tianhua Liu
b
;
1
;
liuth1990@163.com
;
Wei Wei
a
;
Kun Guo
b
;
Ning Yang
a
;
Siqi Tian
a
;
Jia Zhu
a
;
Yinkun Liu
b
;
Wenting Zhou
a
;
Huawei Yang
a
;
lordyhw@Hotmail.com
关键词:
RSK4
;
ribosomal S6 protein kinase 4
;
SF-TAP
;
two STREP II and a single Flag
tag
-based tandem
affinity
purification
;
RSK4 wt
;
RSK4 wild-type
;
RSK4m
;
RSK4 variant
刊名:International Journal of Biological Macromolecules
出版年:2017
4.
A non-cleavable hexahistidine
affinity
tag
at the carboxyl-terminus of the HIV-1 Pr55
Gag
polyprotein alters nucleic acid binding properties
作者:
Maria C. Bewley
a
;
Lisa Reinhart
a
;
Matthew S. Stake
b
;
c
;
Shorena Nadaraia-Hoke
b
;
c
;
Leslie J. Parent
b
;
c
;
;
John M. Flanagan
a
;
jmf27@psu.edu
关键词:
HIV
;
Gag
;
Pr55Gag
;
Nucleic acid binding
;
p6
;
Proteolysis
;
His-
tag
刊名:Protein Expression and Purification
出版年:2017
5.
High-throughput purification of recombinant proteins using self-cleaving intein
tag
s
作者:
M.J. Coolbaugh
a
;
M.J. Shakalli Tang
b
;
D.W. Wood
b
;
wood.750@osu.edu" class="auth_mail" title="E-mail the corresponding author
关键词:
Intein
tag
;
High throughput protein purification
;
Self-cleaving
tag
;
Elastin-like polypeptide
;
Affinity
methods
;
Recombinant protein purification
刊名:Analytical Biochemistry
出版年:2017
6.
In situ
affinity
purification of his-
tag
ged protein A from Bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles
作者:
Johannes Gä
;
dke
a
;
b
;
c
;
1
;
Lennart Kleinfeldt
b
;
d
;
1
;
Chris Schubert
a
;
b
;
c
;
1
;
Manfred Rohde
e
;
Rebekka Biedendieck
c
;
f
;
Georg Garnweitner
b
;
d
;
1
;
Rainer Krull
a
;
b
;
c
;
1
;
r.krull@tu-braunschweig.de
关键词:
CFU
;
colony forming unit
;
DLS
;
dynamic light scattering
;
ELISA
;
enzyme linked immunosorbent assay
;
GNTA-SPION
;
functionalized SPION analyzed in this article
;
OD
;
optical density
;
SPION
;
superparamagnetic iron oxide nanoparticles
;
TGA
;
thermogravimetric analysis
刊名:Journal of Biotechnology
出版年:2017
7.
Development of a simplified purification method for a novel formaldehyde dismutase variant from Pseudomonas putida J3
作者:
Lisa Blaschke
a
;
Wenke Wagner
b
;
Christina Werkmeister
b
;
Marion Wild
b
;
Adrian Gihring
b
;
Steffen Rupp
a
;
b
;
Susanne Zibek
a
;
b
;
susanne.zibek@igb.fraunhofer.de
关键词:
ADH
;
alcohol dehydrogenase
;
AOX
;
alcohol oxidase
;
CV
;
column volume
;
FDH
;
formate dehydrogenase
;
FDM
;
formaldehyde dismutase
;
IMAC
;
immobilized metal
affinity
chromatography
;
MBP
;
maltose binding protein
;
MWCO
;
molecular weight cut-off
刊名:Journal of Biotechnology
出版年:2017
8.
A novel expression vector for the improved solubility of recombinant scorpion venom in Escherichia coli
作者:
Tianqing Han
;
Hongyan Ming
;
Lili Deng
;
Hejie Zhu
;
Zeyu Liu
;
Jinghai Zhang
;
Yongbo Song
;
songyongbo@syphu.edu.cn
关键词:
Buthus martensii Karsch
;
Analgesic activity
;
ANEP
;
Intein
;
trxA
刊名:Biochemical and Biophysical Research Communications
出版年:2017
9.
Achieving ultrasensitive in vivo detection of bone crack with polydopamine-capsulated surface-enhanced Raman nanoparticle
作者:
Chunhuan Jiang
a
;
1
;
Ying Wang
a
;
b
;
1
;
Jiawei Wang
a
;
Wei Song
c
;
weisong@jlu.edu.cn
;
Lehui Lu
a
;
lehuilu@ciac.ac.cn
关键词:
Surface-enhanced Raman scattering
;
Polydopamine
;
Bone crack
;
Ultrasensitive
;
In vivo detection
刊名:Biomaterials
出版年:2017
10.
Application of volcanic ash particles for protein
affinity
purification with a minimized silica-binding
tag
作者:
Mohamed A.A. Abdelhamid
1
;
2
;
Takeshi Ikeda
1
;
ikedatakeshi@hiroshima-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
;
Kei Motomura
1
;
Tatsuya Tanaka
1
;
Takenori Ishida
1
;
Ryuichi Hirota
1
;
Akio Kuroda
1
关键词:
Affinity
purification
;
Arginine
;
Fusion
tag
;
Silica-binding peptide
;
Volcanic ash
;
Bacillus cereus CotB1
刊名:Journal of Bioscience and Bioengineering
出版年:2016
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